酶及其应用的制作方法

本发明涉及相对于参照shc/hac蛋白经过了修饰的角鲨烯何帕烯环化酶/高法呢醇降龙涎香醚环化酶(squalenehopenecyclase/homofarnesolambroxcyclase，shc/hac)衍生酶、构成该shc/hac衍生酶的氨基酸序列、编码该shc/hac衍生物的核苷酸序列、包含编码该shc/hac衍生物的核苷酸序列的载体以及包含编码该shc/hac衍生物的核苷酸序列的重组宿主细胞。本发明还涉及功能性表达编码shc/hac衍生物的核苷酸序列的手段以及利用包含编码shc/hac衍生物和野生型shc/hac的核苷酸序列的重组微生物来制备降龙涎醚(ambrox)、优选(-)-降龙涎醚的方法。
背景技术：
：角鲨烯何帕烯环化酶(squalenehopenecyclases,shc，ec5.4.99.17)是膜结合型原核生物酶，其充当使线性三萜角鲨烯环化为何帕烯和何帕醇的生物催化剂。较早的shc工作集中于嗜热嗜酸性细菌——酸热脂环酸芽孢杆菌(alicyclobacillusacidocaldarius))(以前称为酸热芽孢杆菌(bacillusacidocaldarius))的shc的表征(参见neumann&simon1986,biolchemhoppe-seyler367,723-729；seckler&poralla1986,biochembiophysact356-363以及ochs等人1990,jbacteriol174,298-302)。然而，更近些时候，已经对来自运动发酵单胞菌(zymomonasmobilis)和慢生型大豆根瘤菌(bradyrhizobiumjaponicum)的其它shc进行了纯化并就它们的天然底物(如角鲨烯)和非天然底物(如高法呢醇和柠檬醛)进行了表征(参见例如，wo2010/139710、wo2012/066059以及seitz等人2012，j.molecularcatalysisb:enzymatic84,72-77)。neumann和simon进行的较早工作(1986-如上所述)公开了高法呢醇是酸热脂环酸芽孢杆菌shc(aacshc)的另一种底物。然而，neumann和simon(1986)教导的纯化aacshc的非天然底物高法呢醇的环化速率据报道仅为天然底物角鲨烯的环化速率的3％。在0.25mm至2.0mm的高法呢醇(产物1b)浓度下降龙涎醚(产物2b)的形成速率增加，在存在4mm产物1b的情况下稍微下降。环化速率的差别可能部分归因于天然shc底物角鲨烯(c30碳化合物)的大小是非天然底物高法呢醇(其为c16碳化合物)的两倍这样一个事实。(jp2009060799-花王株式会社(kao))也公开了使用来自酸热脂环酸芽孢杆菌的shc由高法呢醇制备降龙涎醚的方法。而jp2009060799教导了使用包含shc的微生物来合成降龙涎醚的可能性，jp2009060799仅公开了使用从表达shc基因的重组微生物制备的shc液体萃取物而不是通过表达shc基因的整个重组微生物细胞的手段由高法呢醇制备降龙涎醚。使用shc液体萃取物时高法呢醇向降龙涎醚转化的百分比据报道当在ph5.2-6.0下于60℃的温度下进行14小时时为17.5％，但在6.6的ph下进行时仅为6.8％。当使用0.2％高法呢醇(2g/l)底物浓度时，利用shc液体萃取物在ph5.6下于60℃进行64小时，3e,7e-高法呢醇向降龙涎醚转化的百分比据报道为63％。wo2010/139719a2及其美国同族(us2012/0135477a1)描述了具有高法呢醇-降龙涎醚环化酶活性的至少三种shc酶萃取物。当使用10mm(2.36g/l)高法呢醇浓度时，运动发酵单胞菌(zmo)shc和慢生型大豆根瘤菌(bjp)shc酶据报道在16小时的反应中分别显示出41％和22％的高法呢醇转化率，而对于aacshc，其转化率据报道仅为1.2％(假定以相同的高法呢醇浓度)，但未提供实验细节。zmoshc和bjpshc酶萃取物是通过使产生shc酶的大肠杆菌宿主细胞破裂并分离可溶性的shc级分而由表达shc基因的重组微生物而制备的。seitz等人(2012-如上所述)报道了三种shc酶的功能性表达和生化表征，其中两种来自运动发酵单胞菌(zmoshc1和zmoshc2)，一种来自慢生型大豆根瘤菌。据报道，当使用10mm(2.36g/l)的高法呢醇浓度时，使用野生型zmoshc1时观察到高法呢醇向降龙涎醚的“有效”转化(22.95％)，使用野生型zmoshc2时没有高法呢醇向降龙涎醚的转化，而对于aacshc，发现高法呢醇向降龙涎醚的转化相对较低(3.4％)。对于aacshc观察到的高法呢醇向降龙涎醚转化的转化率相对较低的趋势与neumann和simon(1986-如上文所引用的)的结果一致，并且如wo2010/139719a2(也在上面论述)中所公开的。这三种shc酶以细胞悬浮液形式(通过采用冻-融循环使宿主大肠杆菌细胞部分破裂)并且作为部分纯化的膜结合型级分使用。wo2012/066059公开了具有环化酶活性的突变体以及其在以下方法中的用途：用于萜烯的生物催化环化，例如，具体的讲，用于通过使香茅醛环化而制备异蒲勒醇的方法；用于制备薄荷醇的方法以及用于具有萜烯类结构基序的其它化合物的生物催化转化的方法。对各种shc进行序列比对将苯丙氨酸-486(f486)鉴别为强保守性氨基酸残基，并在运动发酵单胞菌shc酶中产生了一系列取代变体。这些取代中的一些导致了活性丧失，而其它则导致了从萜烯底物诸如香茅醛形成新的萜类产物(异蒲勒醇)。2012年seitz的博士论文(http://elib.uni-stuttgart.de/handle/11682/1400)中的报道表明，zmoshc1中的f486y突变使高法呢醇生物转化率降低约1.5倍，从34.8％(野生型zmoshc1)降低至23.9％(突变体zmoshc1f486y)。当测试aacshc中的突变等价物(y420c)时，推测对较大底物的酶活性将降低，而对较小底物的活性将增加。当在与野生型相同的条件下测试该突变体并比较酶活性时，观察到该突变体根本未显示出高法呢醇底物的任何转化。因此其结论是y420氨基酸残基对于aacshc对所有底物的活性是关键的。本领域的其它shc定点诱变(如hoshino和sato2002,chemcommun291-301)聚焦于高度保守区中的突变(如f601)的效果以及它们对天然底物(即角鲨烯或角鲨烯类似物)而不是对非天然底物诸如高法呢醇的效果。概括地说，本领域中涉及用于将高法呢醇成功转化为降龙涎醚的生物转化工艺的有限公开内容仅涉及相对较低浓度/体积的高法呢醇底物(以0.25mm至2mm到10mm或者约0.06g/l至2.36g/l的浓度范围)，使用具有高法呢醇-降龙涎醚环化酶(hac)活性的野生型shc多肽。具有hac活性的shc酶是：(i)通过破坏包含shc酶的大肠杆菌宿主细胞并分离可溶和不可溶的shc液体级分而制备的液体萃取物；(ii)部分纯化的膜级分；或者(iii)表达野生型shc基因并产生shc酶的重组全细胞，该shc酶供用于反应中利用增溶剂将高法呢醇生物转化为降龙涎醚，所述增溶剂包括：(i)反应混合物中的tritonx-100(参见如上文所引用的neumann和simon1986，如上文所引用的seitz等人2012，jp2009060799)；或(ii)牛黄脱氧胆酸盐(如us2012/0135477a1中所公开的)。利用这些野生型shc萃取物和/或表达shc基因的整个重组微生物细胞，据发现所获得的高法呢醇向降龙涎醚转化的转化率依shc酶的来源、高法呢醇原料的量和所采用的反应条件而变化。迄今为止，在所报道的所涉及浓度(0.06-2.36g/l)下，尚未实现利用野生型shc酶将高法呢醇100％转化为降龙涎醚。此外，采用利用定点诱变实验制备的shc衍生物进行的初步研究仅提供了阴性的结果(即降低的高法呢醇转化率)而不是阳性的结果(即提高的转化率)。此外，在公开的研究中仅使用纯化的shc酶萃取物或shc膜结合级分，或者在使用增溶剂诸如tritonx-100或牛黄脱氧胆酸盐的特定反应条件下使用表达野生型shc基因的完整重组微生物细胞。尚未证明包含野生型shc或突变体shc的重组微生物可在使用最佳反应条件下提供更有效的或高性价比的高法呢醇向降龙涎醚的生物转化。因此，期望通过至少改善反应速率、特异性、产率、生产力以及降低费用(通过例如，利用重组全微生物细胞或通过采用将生物催化剂生产步骤和生物转化步骤合并的“一锅法”工艺来简化工艺)来改善所引用的由高法呢醇制备降龙涎醚的已知方法。技术实现要素：本发明在多个方面提供shc/hac衍生物、构成所述shc/hac衍生酶的氨基酸序列、编码shc/hac衍生酶的核苷酸序列、包含编码shc/hac衍生酶的核苷酸序列的载体、包含具有编码shc/hac衍生酶的核苷酸序列的载体的重组宿主细胞以及包含shc/hac衍生酶或野生型shc/hac酶的重组宿主细胞当在特定反应条件下使用时在制备降龙涎醚材料的方法中的应用，所述降龙涎醚材料包含命名为(-)-降龙涎醚的降龙涎醚异构体和降龙涎醚样分子(作为副产物)。与本领域中涉及aacshc的公开内容不同，本申请人第一次证明了，表达shc衍生物基因的整个重组微生物可用于将高法呢醇生物转化为降龙涎醚。此外，表达野生型shc基因和/或产生shc酶的完整重组微生物可用于在本领域未公开的特定反应条件下将高法呢醇生物转化为降龙涎醚。还已出乎意料地发现，在本文所公开的野生型shc/hac参照序列的氨基酸序列中引入最多五个氨基酸改变得到这样的shc/hac衍生酶，该衍生酶与本文所公开的未经修饰的shc参照酶相比，具有显著改善的高法呢醇向降龙涎醚转化的转化率。这些新型的shc/hac衍生酶单独时以及组合时可用于由高法呢醇底物制备降龙涎醚材料，尤其是(-)-降龙涎醚。另一个出乎意料的发现是，除了一种突变体(f601y)之外，本文所公开的shc衍生酶通常在参照shc多肽序列的非保守部分中的氨基酸残基位置处包含非保守取代。这是出乎意料的发现，因为酶保守区中的改变比蛋白质非保守区中的改改变有可能破坏酶的功能(至少就其天然底物而言)。另一出乎意料的发现是，本公开的经表征的shc衍生酶在约35℃而不是约60℃下表现最佳(对非天然底物诸如高法呢醇)，60℃是嗜热微生物诸如aacshc的通常的反应温度。本公开的shc衍生物在较低反应温度下应用于由高法呢醇制备降龙涎醚的方法中对工业规模的降龙涎醚生产周期具有显著的成本优势。本发明的另一个优势是，本公开的shc衍生酶催化有效的生物转化过程，该生物转化过程在用与本领域先前描述的浓度(如125g/l的eeh)比较相对较高(如，约50倍)的高法呢醇底物浓度优化时可导致高法呢醇底物100％转化，而参照wtshc蛋白仅转化约10％的相同底物，即使是在高的酶/细胞浓度下时也是如此。所引用的现有技术中的公开内容全部涉及使用包含shc的纯化膜萃取物或纯化shc萃取物(从表达shc基因的微生物制备)或在特定的生物转化反应条件下(如使用特定的增溶剂)使用表达野生型shc基因的重组微生物。即使那样，在低很多的eeh浓度下也未见报道100％的高法呢醇转化率。另外，尚未报道“一锅法”反应，其中在第一步骤中，在同一容器中使重组细胞生长并产生shc酶，随后将eeh转化为(-)-降龙涎醚。本发明的另一个优点是，产生shc衍生酶的重组宿主细胞显示出高的初始反应速率，这使得能在仅使用相对较低量的生物催化剂的同时在相对较短的时间周期内产生大量的产物。简而言之，包含野生型shc/hac或特定shc/hac衍生酶的重组微生物在特定生物转化反应条件下的选择和有效表达以及应用导致更有效的生物转化过程。可分离最终产物((-)-降龙涎醚)并容易地纯化。不像所引用的现有技术，所述shc/hac衍生酶不作为纯的酶使用而是在完全细胞情形下使用(作为生物催化剂)，这是更加节省成本且更加用户友好和环境友好的方法，因为不需要另外的酶纯化和分离步骤。概括地说，本公开提供了用于在重组微生物菌株中制备降龙涎醚的生物转化/生物转换方法，其中该方法：(i)在经济上有吸引力，(ii)环境友好，以及(iii)导致选择性地产生(-)-降龙涎醚作为优势化合物，该化合物在选择性结晶条件下有效地与其它副产物分离，这些副产物不会促成最终产物的嗅觉品质。具体实施方式如本文所用，术语“shc”意指来自表10-12中所列来源任一者的角鲨烯何帕烯环化酶。在优选的实施方案中，术语shc包括如basfwo2010/139719、us2012/01345477a1、seitz等人(2012，如上文所引用的)和seitz(2012博士论文，如上文所引用的)中所公开的运动发酵单胞菌shc酶和酸热脂环酸芽孢杆菌shc酶。为了便于参考，名称“aacshc”用于酸热脂环酸芽孢杆菌shc，而名称“zmoshc”用于运动发酵单胞菌shc，名称“bjpshc”用于慢生型大豆根瘤菌shc。这些序列相对于野生型aacshc以及彼此之间的序列同一性百分比(可根据所用的算法而变动)在表18和表19中列出。由hoshino和sato(2002，如上文所引用的)进行的野生型shc序列的比对表明，在全部四种序列中检测到多个基序并且这些基序由核心序列gln-x-x-x-gly-x-trp组成，该核心序列在运动发酵单胞菌和酸热脂环酸芽孢杆菌二者的shc序列中出现六次(参见reipen等人1995,microbiology141,155-161的图3)。hoshino和sato(2002，如上文所引用的)报道说，shc中通常富含芳族氨基酸并且在shc中发现两个特征性的基序：一个为由特定氨基酸基序[(k/r)(g/a)x2-3(f/y/w)(l/iv)3x3qx2-5gxw]代表的qw基序，另一个为dxddta基序。wendt等人(1997,science277,1811-1815以及1999,jmolbiol286,175-187)报道了对酸热脂环酸芽孢杆菌shc的x射线结构分析。dxddta基序看起来与shc活性位点相关。来自现有技术的示例性序列比对显示了反复出现的多个基序，如本文中的图2(来自hoshino和sato(2002，如上文所引用的))和图3(来自seitz博士论文(2012))中所提供的。本文所用的参照(或野生型)aacshc蛋白是指seqidno.1中所公开的aacshc蛋白。本公开的参照aacshc酶具有高法呢醇降龙涎香醚环化酶(hac)的活性，该活性可用于通过shc与高法呢醇底物的生物催化反应制备降龙涎醚衍生物。参照aacshc的主要反应是使线性或非线性底物诸如高法呢醇环化而产生降龙涎醚。降龙涎醚如本文所用，术语“降龙涎醚”包括式(i)的(-)-降龙涎醚以及立体异构体纯形式的或与下面式(ii)、(iv)和/或(iii)的分子中的至少一种或多种的混合物形式的(-)-降龙涎醚。(-)-降龙涎醚(-)-降龙涎醚在商业上称为降龙涎醚(芬美意公司(firmenich))、降龙涎醚an(汉高公司(henkel))、ambrofix(奇华顿公司(givaudan))、amberlyn(奎斯特公司(quest))、cetaloxlaevo(芬美意公司)、ambermor(埃姆公司(aromor))和/或norambrenolideether(太平洋公司(pacific))。(-)-降龙涎醚是一种在工业上重要的芳香化合物，已被用于香料行业很久了。(-)-降龙涎醚的特殊受欢迎的感官有益效果来自该(-)立体异构体而不是(+)立体异构体。(-)立体异构体的气味被描述为类似麝香、木香、温暖或琥珀味，而(+)-降龙涎醚对映体具有相对较弱的气味。降龙涎醚样产品的气味和气味阈值也是不同的。虽然各种富含(-)-降龙涎醚的材料可商购获得，但希望制备高度富含(-)-降龙涎醚的材料，理想的是纯的(-)-降龙涎醚。(-)-降龙涎醚的制备(-)-降龙涎醚可根据下面所描述的制备工艺由香紫苏内酯制备。香紫苏醇是从天然植物香紫苏萃取的产物。然而，由于该工艺中使用天然原料，所以存在潜在的问题，问题在于其涉及多级反应，其操作是迂回的，原料供应的质量及稳定性可能并不会总令人满意，并且该反应可能不是环境友好的，因为在(+)-香紫苏醇氧化降解步骤中使用氧化剂诸如铬酸或高锰酸盐。(-)-降龙涎醚还使用不同的路线由高法呢醇合成。举个例子，可通过溴化、氰化以及水解橙花叔醇而得到高法呢酸，然后还原而获得高法呢醇。作为另一种选择，可由法呢醇、氯化法呢酯、β-法呢烯或其它底物获得高法呢醇。β-法呢烯可直接转换为e,e-高法呢醇(eeh)或经由e,e-高法呢酯(其然后转换为eeh)间接转换为eeh。关于由不同底物制备(-)-降龙涎醚的综述可见于us2012/0135477a1、wo2010/139719、us2013.0273619a1、wo2013/156398a1和seitz博士论文(2012，如上文所引用的)以及schaefer2011(chemieunsererzeit45,374-388)。虽然高法呢醇可能作为四种异构体((3z,7z)、(3e,7z)、(3z,7e)和(3e,7e)异构体)的混合物存在，但根据文献似乎(-)-降龙涎醚仅从(3e,7e)高法呢醇获得(参见neumann和simon(1986)，如上文所引用的)。如本文所用，提及(3e,7e)高法呢醇即提及e,e-高法呢醇，其也命名为eeh。us2012/0135477a1报道了使用zmoshc(seqidno.2)使(3z,7e)转化为(-)-降龙涎醚(参见实施例2-4)，但是根据schaefer(2011)(如上文所引用的)中的公开内容，(7e,3z)仅转化为如上文所概述的9b-表-降龙涎醚(即化合物iii)而不转化为(-)-降龙涎醚。如本文所用，提及(3z,7e)高法呢醇即提及e,z-高法呢醇，其也命名为ezh。在一些实施方案中，优选该高法呢醇原料包含(3e,7e)和(3z,7e)的混合物，该混合物在本文称为ee:ez立体异构体混合物(具体参见实施例和表20)。高法呢醇的ee:ez立体异构体混合物的cas编号为35826-67-6。如实施例所展示的(如参见实施例5、7、9、10、11、18、19和20)，在某些实施方案中，该高法呢醇给料/原料是异构体的混合物。因此，在一些实施方案中,该高法呢醇原料还可包含四种异构体ee:ez:zz:ze的混合物，这四种异构体对应于(3e,7e)和(3z,7e)、(3z,7z)和(3e,7z)。在一些实施方案中，优选该高法呢醇原料选自以下组中的一者或多者:[(3z,7z)、(3e,7z)、(3z,7e)和(3e,7e)]、[(3z,7e)和(3e,7e)]、[(3z,7e)、(3e,7z)]和/或[(3e,7e)和(3e,7z)]。优选地，该高法呢醇原料选自以下组中的一者或多者：[(3e,7e)、(3z,7e)]和/或[(3z,7e)、(3e,7e)和(3e,7z)]。因此，在某些实施方案中，eeh:ezh的比率为约100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29；70:30；69:31；68:32；67:33；66:34；65:35；64:36；63:37；62:38；61:39；60:40；59:41；58:42；57:43；56:44；55:45；54:46；53:47；52:48；51:49；或约50:50。在一些实施方案中，优选该高法呢醇原料包含>90％的e,e-高法呢醇(eeh)。在其它实施方案中，该高法呢醇原料包含86:14重量比的ee:ez。在某些实施方案中，该高法呢醇原料包含80:20重量比的ee:ez。在某些实施方案中，该高法呢醇原料包含70:30重量比的ee:ez。在另外的实施方案中，该高法呢醇原料包含69:31重量比的ee:ez。在一些实施方案中,该高法呢醇原料由四种异构体ee:ez:zz:ze的混合物组成或基本上由所述混合物组成，这四种异构体对应于(3e,7e)和(3z,7e)、(3z,7z)和(3e,7z)。在一些实施方案中，优选该高法呢醇原料由选自以下组中的一者或多者的异构体混合物组成或基本上由所述混合物组成：[(3z,7z)、(3e,7z)、(3z,7e)和(3e,7e)]、[(3z,7e)和(3e,7e)]、[(3z,7e)、(3e,7z)]和/或[(3e,7e)和(3e,7z)]。优选地，该高法呢醇原料由选自以下组中的一者或多者的异构体混合物组成或基本上由其组成：[(3e,7e)、(3z,7e)]和/或[(3z,7e)、(3e,7e)和(3e,7z)]。因此，在某些实施方案中，eeh:ezh异构体的比率由以下eeh:ezh比率组成或基本上由以下eeh:ezh比率组成：约100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29；70:30；69:31；68:32；67:33；66:34；65:35；64:36；63:37；62:38；61:39；60:40；59:41；58:42；57:43；56:44；55:45；54:46；53:47；52:48；51:49；或约50:50。在一些实施方案中，优选高法呢醇原料由>90％的e,e-高法呢醇(eeh)组成或基本上由其组成。在其它实施方案中，该高法呢醇原料由86:14重量比的ee:ez组成或基本上由其组成。在某些实施方案中，该高法呢醇原料由80:20重量比的ee:ez组成或基本上由其组成。在某些实施方案中，该高法呢醇原料由70:30重量比的ee:ez组成或基本上由其组成。在另外的实施方案中，该高法呢醇原料由69:31重量比的ee:ez组成或基本上由其组成。在本公开的实施方案中，使用shc/hac衍生酶制备降龙涎醚。shc/hac衍生物如本文所用，术语“shc/hac衍生物”意指shc/hac衍生物的氨基酸序列是经修饰的氨基酸序列或变体氨基酸序列，该序列与根据至少seqidno.1或seqidno.2或seqidno.3或seqidno.4的参照(或野生型)shc序列的氨基酸序列相比发生改变。一般而言，shc/hac衍生物包含具有至少一个改变的shc改变形式，该改变修饰(如增加)该酶对其底物(如eeh)的活性。对本公开的shc/hac衍生物测试它们的高法呢醇降龙涎香醚环化酶活性。因此，使高法呢醇转化为降龙涎醚的这些shc/hac衍生物在本文称为hac衍生物以及shc衍生物。虽然对源于酸热脂环酸芽孢杆菌、运动发酵单胞菌、慢生型大豆根瘤菌微生物菌株来源的酶已提供了示例性的shc/hac衍生物，本公开还涵盖来自其它微生物菌株来源的等同shc/hac衍生物，所述来自其它微生物菌株来源的等同shc/hac衍生物包括但不限于来自荚膜甲基球菌(methylococcuscapsulatus)、桤木弗兰克氏菌(frankiaalni)、巴氏醋酸杆菌(acetobacterpasteurianum)和梨形四膜虫(tetrahymenapyriformis)的shc/hac酶(参见例如，wo2010/139719、us2012/01345477、wo2012/066059以及表10-12)。如本文所用，术语“氨基酸改变”意指相对于参照氨基酸序列(例如，seqidno.1或seqidno.2或seqidno.3或seqidno.4的野生型(wt)氨基酸序列)的氨基酸序列，在两个氨基酸之间插入一个或多个氨基酸、缺失一个或多个氨基酸或用一个或多个不同的氨基酸取代(其可以是保守取代或非保守取代)一个或多个氨基酸。可通过将shc/hac衍生物氨基酸序列的氨基酸序列与参照氨基酸序列(例如，seqidno.1或seqidno.2或seqidno.3或seqidno.4的野生型(wt)氨基酸序列)的氨基酸序列相比较而容易地鉴别氨基酸改变。示例性的wtshc氨基酸序列比对在图1-4以及表18和表19中提供。可例如基于所涉及的氨基酸残基的极性、电荷、大小、溶解性、疏水性、亲水性和/或两亲性性质的相似性作出保守性氨基酸取代。上面概述的20种天然存在的氨基酸可分成以下六个标准氨基酸组：(1)疏水性氨基酸：met、ala、val、leu、ile；(2)中性亲水性氨基酸：cys、ser、thr、asn、gln；(3)酸性氨基酸：asp、glu；(4)碱性氨基酸：his、lys、arg；(5)影响链取向的残基：gly、pro；以及(6)芳族氨基酸：trp、tyr、phe。因此，如本文所用，术语“保守取代”意指氨基酸替换成上面所示的六个标准氨基酸组的同一组内所列的另一氨基酸。例如，asp替换为glu可在经过这样修饰的多肽中保留负电荷。此外，甘氨酸和脯氨酸根据它们破坏α-螺旋的能力而可以互相取代。上面六个组内的某些优选的保守取代是以下亚组内的替换：(i)ala、val、leu和ile；(ii)ser和thr；(ii)asn和gln；(iv)lys和arg；以及(v)tyr和phe。鉴于已知的遗传密码以及重组和合成dna技术，熟练的科学家可容易构建编码保守氨基酸变体的dna。如本文所用，“非保守取代”或“非保守氨基酸替换”定义为氨基酸替换为如上所示的六个标准氨基酸组(1)至(6)的不同组中所列的另一氨基酸。通常本公开的shc/hac衍生物利用非保守取代制备，所述非保守取代使所公开的shc/hac衍生物的生物学功能(如hac活性)发生改变。为了易于参照，iupac-iub生物化学命名委员会推荐的单字母氨基酸符号给出如下。还提供了三字母代码以供参照。氨基酸改变诸如氨基酸取代可利用已知的重组基因技术方案引入，所述重组基因技术包括pcr、基因克隆、cdna的定点诱变、宿主细胞的转染以及体外转录，其可用于将这类改变引入wtshc序列中从而得到shc/hac衍生酶。然后可针对shc/hac功能活性对衍生物进行筛选。shc/hac衍生酶本发明提供shc/hac衍生物并且描述具有高法呢醇降龙涎香醚环化酶(hac)活性的酶，其包含的氨基酸序列相对于根据至少seqidno.1或seqidno.2或seqidno.3或seqidno.4的参照(或野生型)shc序列的氨基酸序列具有约1至约50个独立选自取代、缺失或插入的突变。在多个实施方案中，与未显示这种缺失/添加的参照shc酶相比，突变或突变组合增强了所述shc/hac衍生物将高法呢醇转换为降龙涎醚的活性。如本文所述的蛋白质建模可用于引导在shc参照序列中的这种取代、缺失或插入。例如，可利用aacshc的坐标生成shc氨基酸序列的结构模型(例如如在图19和20中所示)。如本文所展示的，这种同源建模可用于指导shc酶将高法呢醇转化为(-)-降龙涎醚的改善。因而，在多个实施方案中，相对于根据至少seqidno.1或seqidno.2或seqidno.3或seqidno.4的参照(或野生型)shc序列的氨基酸序列，shc/hac衍生物可具有约1至约45个突变、约1至约40个突变、约1至约35个突变、约1至约30个突变、约1至约25个突变、约1至约20个突变、约1至约15个突变、约1至约10个突变、或约1至约5个突变。在多个实施方案中，相对于根据至少seqidno.1或seqidno.2或seqidno.3或seqidno.4的参照(或野生型)shc序列的氨基酸序列，shc/hac衍生物包含具有至少5或至少10个突变但不超过约20或30个突变的序列。在多个实施方案中，相对于参照shc(例如，seqidno.1或2或3或4)，shc衍生物可具有约1个突变、约2个突变、约3个突变、约4个突变、约5个突变、约6个突变、约7个突变、约8个突变、约9个突变、约10个突变、约11个突变、约12个突变、约13个突变、约14个突变、约15个突变、约16个突变、约17个突变、约18个突变、约19个突变、约20个突变、约21个突变、约22个突变、约23个突变、约24个突变、约25个突变、约26个突变、约27个突变、约28个突变、约29个突变、约30个突变、约31个突变、约32个突变、约33个突变、约34个突变、约35个突变、约36个突变、约37个突变、约38个突变、约39个突变、约40个突变、约41个突变、约42个突变、约43个突变、约44个突变、约45个突变、约46个突变、约47个突变、约48个突变、约49个突变、或约50个突变。在这些或其它实施方案中，shc/hac衍生物可包含这样的氨基酸序列，该序列与wtshc(例如，seqidno.1或seqidno.2或seqidno.3或seqidno.4)或者在参照序列(参见例如表18和表19，其中示出了aacshc(seqidno.1)与其它shc序列(如wo2010/139719的zmoshc)之间有至少34-52％的同一性)具有至少约50％序列同一性、至少约55％序列同一性、至少约60％序列同一性、至少约65％序列同一性、至少约70％序列同一性、至少约75％序列同一性、至少约80％序列同一性、至少约85％序列同一性、或至少90％序列同一性、或至少91％序列同一性、或至少92％序列同一性、或至少93％序列同一性、或至少94％序列同一性、或至少95％序列同一性、或至少96％序列同一性、或至少97％序列同一性、或至少98％序列同一性、或至少99％序列同一性。在多个实施方案中，与野生型酶相比shc变体具有较高的将高法呢醇转换为降龙涎醚的活性，诸如与参照野生型酶(例如，seqidno.1或seqidno.2或seqidno.3或seqidno.4)相比，在与高法呢醇底物接触后(-)-降龙涎醚的产量更高。例如，shc/hac衍生物可包含这样的氨基酸序列，该氨基酸序列与参照shc(例如，seqidno.1或2或3或4)或者在参照序列之间(参见例如表18和表19，其中示出了aacshc(seqidno.1)与其它shc序列(如wo2010/139719的zmoshc)有至少34-52％的同一性)具有：约50％同一性、约51％同一性、约52％同一性、约53％同一性、约54％同一性、约55％同一性、约56％同一性、约57％同一性、约58％同一性、约59％同一性、约60％同一性、约61％同一性、约62％同一性、约63％同一性、约64％同一性、约65％同一性、约66％同一性、约67％同一性、约68％同一性、约69％同一性、约70％同一性、约71％同一性、约72％同一性、约73％同一性、约74％同一性、约75％同一性、约76％同一性、约77％同一性、约78％同一性、约79％同一性、约80％同一性、约81％同一性、约82％同一性、约83％同一性、约84％同一性、约85％同一性、约86％同一性、约87％同一性、约88％同一性、约89％同一性、约90％同一性、约91％序列同一性、约92％序列同一性、约93％序列同一性、约94％序列同一性、约95％序列同一性、约96％序列同一性、约97％序列同一性、约98％序列同一性、或约99％序列同一性。已经测试了shc酶活性的各种shc/hac衍生物在表1-9中的一者或多者中列出。因而，在多个实施方案中，shc/hac衍生物可具有至少约1、至少约2、至少约3、至少约4、至少约5、至少约6、至少约7、至少约8、至少约9或至少约10个选自表1-9中的一者或多者的突变。在一些实施方案中,shc/hac衍生物是经修饰的shc多肽，该多肽包含相比于根据seqidno.1的野生型/参照氨基酸序列具有最多4个突变且相对于seqidno.1至少包含与f129l和/或i432t中的至少任一者或多者相组合的取代f601y或m132r的氨基酸序列，并且任选包含支持在大肠杆菌中的表达和活性的前导序列。在其它实施方案中，shc/hac衍生物是经修饰的shc多肽，该多肽包含与根据seqidno.1的野生型/参照氨基酸序列(或其例如经修饰以在大肠杆菌中表达的对应物)相比具有最多8个突变且相对于seqidno.1在选自位置77、92、129、132、224、432、579、601和605的位置中包含一个或多个氨基酸改变的氨基酸序列，其中该shc/hac衍生物相对于seqidno.1具有经修饰的(如增加的)酶活性。在一个实施方案中，shc衍生物相对于seqidno.1包含一个或多个取代，所述取代选自由以下组成的突变体群组：t77x、i92x、f129x、m132x、a224x、i432x、q579x、f601y和f605w，其中：t77x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i92x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f129x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。m132x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。a224x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i432x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。q579x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f601x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f605x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。在一个实施方案中，shc衍生物相对于seqidno.1包含一个或多个取代，所述取代选自由以下组成的突变体群组：t77a、i92v、f129l、m132r、a224v、i432t、q579h、f601y和f605w。在另一个实施方案中，shc衍生物相对于seqidno.2包含一个或多个取代，所述取代选自由以下组成的突变体群组：s129x、v145x、f182x、y185x、g282x、i498x、h646x和f698x，其中：s129x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。v145x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f182x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。y185x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。g282x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i498x具有选自以下的x：a、b、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。h646x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f668x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f698x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。在一个实施方案中，如表2中所示，shc衍生物相对于seqidno.2包含一个或多个取代，所述取代选自由以下组成的突变体群组：s129a、v145v、f182l、y185r、g282v、i498t、h646h、f668y和f698x。在一另外的实施方案中，shc衍生物相对于seqidno.3包含一个或多个取代，所述取代选自由以下组成的突变体群组：g85x、v100x、f137x、i140x、v233x、i450x、n598x、f620x和f624x，其中：g85x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。v100x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f137x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i140x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。v233x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i450x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。n598x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f620x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f624x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。在一个实施方案中，如表3和表3a中所示，shc衍生物相对于seqidno.3包含一个或多个取代，所述取代选自由以下组成的突变体群组：g85a、v100v、f137l、i140r、v233v、i450t、n598h、f620y和f624w。在一另外的实施方案中，shc衍生物相对于seqidno.4包含一个或多个取代，所述取代选自由以下组成的突变体群组：a88x、v104x、f141x、y144x、v241x、i459x、m607x、f628x和f658x，其中：a88x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。v104x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f141x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。y144x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。v241x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。i459x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。m607x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f628x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。f658x具有选自以下的x：a、c、d、e、f、g、h、i、k、l、m、n、p、q、r、s、t、v、w或y。在一另外的实施方案中，如表4中所示，shc衍生物相对于seqidno.4包含一个或多个取代，所述取代选自由以下组成的组：a88a、v104v、f141l、y144r、v241v、i459t、m607h、f628y和f658w。shc衍生物组合在一个实施方案中，如表5中所示，shc衍生物相对于seqidno.1包含一个或多个取代，所述取代选自由以下组成的突变体群组：t77a、f129l、m132r、i92v、a224v、i432t、q579h和f601y。在一个实施方案中，如表6中所示，shc衍生物包含相对于seqidno.2的一个或多个取代，所述取代选自由以下组成的突变体群组：s129a、v145vf182l、y185r、g282v、i498t、h646h和f668y。在一个实施方案中，如表7中所示，shc衍生物相对于seqidno.3包含一个或多个取代，所述取代选自由以下组成的突变体群组：g85a、v100vf137l、i140r、v233v、i450t、n598h和f620y。在一另外的实施方案中，如表8中所示，shc衍生物相对于seqidno.4包含一个或多个取代，所述取代选自由以下组成的组：a88a、v104v、f141l、y144r、v241v、i459t、m607h和f628y。表1：相对于野生型aacshc(seqidno.1)和来自hoshino和sato(2002，如上文所引用的)的图1进行编号的shc突变的汇总表2：相对于野生型aacshc(seqidno.1)进行编号的shc突变以及aacshc与zmoshc1(seitz等人2012，如上文所引用的)序列比对的汇总，补充数据表表3：相对于野生型aacshc(seqidno.1)进行编号的shc突变以及aacshc与zmoshc2(seitz等人2012，如上文所引用的)比对的汇总，补充数据表表3a：相对于野生型aacshc(seqidno.1)编号的shc突变以及aacshc与merkofer博士论文(2004)(参见http://elib.uni-stuttgart.de/handle/11682/1400)的shc比对图中的序列no.20的序列比对的汇总表4：相对于野生型aacshc(seqidno.1)编号的shc突变以及aacshc与bjpshc(wo2010/139719中的seqidno.5)的序列比对的汇总表4a：利用表21a-21j中提供的序列比对(其中将wtaacshc(seqidno.1)与seqidno.149、151、153、155、157、159(如下文所确定的)中的任一者比对)，可鉴别对应于wtaacshc(seqidno.1)中的t77、i92、f129、m132、a224、i432、q579和f601的氨基酸残基和位置并测试shc/hac活性表5：根据野生型aacshc(seqidno.1)编号的shc突变组合的汇总表6：根据野生型azmoshc1序列(seqidno.2)编号的shc突变组合的汇总表7：根据野生型zmoshc2序列(seqidno.3)编号的shc突变组合的汇总表8：根据野生型bjpshc(seqidno.4)编号的shc突变组合的汇总表9：示出了相对于wtaacshc(seqidno.1)、wtzmoshc1(seqidno.2)、wtzmoshc2(seqidno.3)和bjpshc(seqidno.4)的共同shc突变在一个优选的实施方案中，相对于seqidno.1，shc衍生物至少包含与f129l和/或i432t中的至少任一者或多者相组合的取代f601y或m132r。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc3的shc衍生物包含以下取代：f601y。hoshino和sato(2002，如上文所引用的)将f601鉴别为原核和真核生物物种中高度保守的氨基酸残基。据报道，对环氧角鲨烯底物(不是角鲨烯)，shc衍生物f601y显示出大大增加的vmax。然而当使用角鲨烯时，相对于野生型aacshc，f601y显示出亲和力降低(即更高的km)和催化效率/活性(kcat/km)降低。hoshino和sato(2002，如上文所引用的)中未提供当将高法呢醇作为酶底物用于f601y突变体时aacshc效力的数据。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc10的shc衍生物包含以下取代：f129l。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc30的shc衍生物包含以下取代：f601y和f129l。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc26的shc衍生物包含以下取代：m132r和i432t。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为215g2的shc衍生物包含以下取代：m132r、i432t和a224v。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc32的shc衍生物包含以下取代：f601y、m132r和i432t。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc31的shc衍生物包含以下取代：f129l、m132r和i432t。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为shc33的shc衍生物包含以下取代：f601y、f129l、m132r和i432t。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为101a10的shc衍生物包含以下取代：f601y和q579h。与参照shc蛋白(seqidno.1)相比，本公开中提供的称为111c8的shc衍生物包含以下取代：t77a+i92v和f129l。在一个优选的实施方案中，相对于seqidno.2，shc衍生物至少包含与f182l和/或i498t中的至少任一者或多者相组合的取代f668y或y185r。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc3zm1的shc衍生物包含以下取代：f668y。hoshino和sato(2002，如上文所引用的)将f601鉴别为原核和真核生物物种中高度保守的氨基酸残基。据报道，对环氧角鲨烯底物(不是角鲨烯)，shc衍生物f601y显示出大大增加的vmax。然而，当使用角鲨烯时，相对于野生型aacshc，f601y显示出亲和力降低(即更高的km)和催化效率/活性(kcat/km)降低。hoshino和sato中未提供当将高法呢醇作为酶底物用于f601y突变体时aacshc效力的数据。相当于zmoshc1中的f601y的shc衍生物是f668y。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc10zm1的shc衍生物包含以下取代：f182l。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc30zm1的shc衍生物包含以下取代：f668y和f182l。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc26zm1的shc衍生物包含以下取代：y185r和i498t。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为215g2zm1的shc衍生物包含以下取代：y185r、i498t和g282v。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc32zm1的shc衍生物包含以下取代：f668y、y185r和i498t。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc31zm1的shc衍生物包含以下取代：f182l、y185r和i498t。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为shc33zm1的shc衍生物包含以下取代：f668y、f182l、y185r和i498t。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为101a10zm1的shc衍生物包含以下取代：f668y和h646h。与参照shc蛋白(seqidno.2)相比，本公开中提供的称为111c8zm1的shc衍生物包含以下取代：s129a+v145v和f182l。在一个优选的实施方案中，相对于seqidno.3，shc衍生物至少包含与f137l和/或i450t中的至少任一者或多者相组合的取代f620y或i140r。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc3zm2的shc衍生物包含以下取代：f620y。hoshino和sato(2002，如上文所引用的)将f601鉴别为原核和真核生物shc种类中高度保守的氨基酸残基。据报道，对环氧角鲨烯底物(不是角鲨烯)，aacshc衍生物f601y显示出大大增加的vmax。然而，当使用角鲨烯时，相对于野生型aacshc，f601y显示出亲和力降低(即更高的km)和催化效率/活性(kcat/km)降低。hoshino和sato(2002)中未提供当将高法呢醇作为酶底物用于f601y突变体时aacshc效力的数据。相当于zmoshc2中的f601y的shc衍生物是f620y。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc10zm2的shc衍生物包含以下取代：f137l。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc30zm2的shc衍生物包含以下取代：f620y和f137l。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc26zm2的shc衍生物包含以下取代：i140r和i450t。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为215g2zm2的shc衍生物包含以下取代：i140r、i450t和v233v。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc32zm2的shc衍生物包含以下取代：f620y、i140r和i450t。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc31zm2的shc衍生物包含以下取代：f137l、i140r和i450t。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为shc33zm2的shc衍生物包含以下取代：f620y、f137l、i140r和i450t。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为101a10zm2的shc衍生物包含以下取代：f620y和n598h。与参照shc蛋白(seqidno.3)相比，本公开中提供的称为111c8zm2的shc衍生物包含以下取代：g85a+v100v和f137l。在一个优选的实施方案中，相对于seqidno.4，shc衍生物至少包含与f141l和/或i459t中的至少任一者或多者相组合的取代f628y或y144r。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc3bjp的shc衍生物包含以下取代：f628y。hoshino和sato(2002，如上文所引用的)将f601鉴别为原核和真核生物种类中高度保守的氨基酸残基。据报道，对环氧角鲨烯底物(不是角鲨烯)，shc衍生物f601y显示出大大增加的vmax。然而，当使用角鲨烯时，相对于野生型aacshc，f601y显示出亲和力降低(即更高的km)和催化效率/活性(kcat/km)降低。hoshino和sato中未提供当将高法呢醇作为酶底物用于f601y突变体时aacshc效力的数据。相当于bjpshc中的f601y的shc衍生物是f628y。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc10bjp的shc衍生物包含以下取代：f141l。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc30bjp的shc衍生物包含以下取代：f628y和f141l。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc26bjp的shc衍生物包含以下取代：y144r和i459t。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为215g2bjp的shc衍生物包含以下取代：y144r、i459t和v241v。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc32bjp的shc衍生物包含以下取代：f628y、y144r和i459t。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc31bjp的shc衍生物包含以下取代：f141l、y144r和i459t。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为shc33bjp的shc衍生物包含以下取代：f628y、f141l、y144r和i459t。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为101a10bjp的shc衍生物包含以下取代：f628y和m607h。与参照shc蛋白(seqidno.4)相比，本公开中提供的称为111c8bjp的shc衍生物包含以下取代：a88a+v104v和f141l。氨基酸序列在一些实施方案中，aacshc/hac衍生物包含seqidno.5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39和/或171中的一者或多者中所列出的多肽中的一者或多者。优选地，本公开的aacshc/hac衍生物具有选自以下的氨基酸序列：seqidno.21、seqidno.23、seqidno.25seqidno.27、seqidno.29、seqidno.31、seqidno.33、seqidno.35、seqidno.37、seqidno.39和/或seqidno.171。在其它实施方案中，zmoshc1/hac衍生物包含seqidno.41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75和/或173中的一者或多者中所列出的多肽中的一者或多者。优选地，本公开的zmoshc1/hac衍生物具有选自以下的氨基酸序列：seqidno.57、seqidno.59、seqidno.61seqidno.63、seqidno.65、seqidno.67、seqidno.69、seqidno.71、seqidno.73、seqidno.75和/或seqidno.173。在另外的实施方案中，zmoshc2/hac衍生物包含seqidno.77、seqidno.79、seqidno.81、seqidno.83、seqidno.85、seqidno.87、seqidno.89、seqidno.91、seqidno.93、seqidno.95、seqidno.97、seqidno.99、seqidno.101、seqidno.103、seqidno.105、seqidno.107、seqidno.109、seqidno.111和/或seqidno.175中的一者或多者中所列出的多肽中的一者或多者。在另外的实施方案中，bjpshc/hac衍生物包含以下中的一者或多者中所列出的多肽中的一者或多者：seqidno.113、seqidno.115、seqidno.117、seqidno.119、seqidno.121、seqidno.123、seqidno.125、seqidno.127、seqidno.129、seqidno.131、seqidno.133、seqidno.135、seqidno.137、seqidno.139、seqidno.141、seqidno.143、seqidno.145、seqidno.147和/或seqidno.177。序列比对由于shc参照序列(例如，aacshc、zmoshc1、zmoshc2和bjpshc多肽序列)长度不同，参照aacshc序列(seqidno.1)的位置x处的氨基酸残基对应zmoshc1参照序列(seqidno.2)上的不同氨基酸位置b、zmoshc2参照序列(seqidno.3)上的不同氨基酸位置j以及bjpshc参照序列(seqidno.4)上的不同氨基酸位置z。此外，shc参照序列的改变也可相对该参照shc序列而言修饰shc衍生物序列。术语“位置”是指参照shc蛋白中存在的通过特定氨基酸编号所确定的具体氨基酸残基。通过插入或缺失氨基酸来使shc参照蛋白改变可导致该参照shc氨基酸序列与shc衍生物氨基酸序列之间的编号不一样。举个例子，如果在参照shc蛋白的氨基酸509与氨基酸510之间插入一个氨基酸，则该插入后面的氨基酸将在shc衍生蛋白中具有编号511，而在shc参照蛋白中其将保持编号510。用于测定wtshc/hac和shc/hac衍生物活性的测定法本文描述了用于测定和定量wtshc/hac和/或shc/hac衍生酶活性的测定法，它们是本领域已知的。举个例子，wtshc/hac和/或shc/hac衍生物活性可通过这样来测定：将纯化的shc/hac酶或来自已产生shc/hac酶的宿主细胞的萃取物或完整的重组宿主生物体与适当的底物在适当的条件下温育并对反应产物进行分析(如通过气相色谱(gc)或hplc分析)。关于shc/hac和/或shc/hac酶活性测定法以及反应产物分析的进一步细节在实施例中提供。这些测定法包括在重组宿主细胞(如大肠杆菌)中产生shc衍生物。如本文所用，术语“活性”意指酶与底物反应而提供靶产物的能力。可以在通过靶产物随时间增加、底物(或原料)随时间减少或通过这些参数的组合随时间变化来测定的活性测定法中测定活性。本公开的shc/hac衍生物表征为它们将高法呢醇生物转化为(-)-降龙涎醚并且展示出生物活性诸如hac活性的能力。本文所用的“生物活性”是指多肽可能展现的任何活性，包括但不限于：酶活性；结合另一化合物的活性(如结合至另一多肽，尤其是结合至受体，或者结合至核酸)；抑制活性(例如酶抑制活性)；激活活性(如酶激活活性)；或毒性效应。并非要求该变体或衍生物展现出的这种活性的程度与亲本多肽一样。如果变体展现出的相关活性的程度为亲本多肽的活性的至少10％，则该变体被认为是本申请范围内的变体。同样，如果衍生物展现出的相关生物活性的程度为亲本多肽的活性的至少10％，则该衍生物被认为是本申请范围内的衍生物(因为术语衍生物和变体在整个本公开中可互换使用)。在其它实施方案中，本公开的shc/hac衍生物显示出比参照shc蛋白更好的靶产率。术语“靶产率”是指每克原料的可回收产物克数(其可计算为摩尔转化率百分数)。在另外的实施方案中，相对于参照shc蛋白，本公开的shc/hac衍生物显示出修饰的(如增加的)靶生产力。术语“靶生产力”是指每小时生物转化时间(即添加底物后的时间)每升发酵容量的可回收靶产物的量(克数)。在另外的实施方案中，与参照shc蛋白相比，本公开的shc/hac衍生物显示出改变的靶产率因子。术语“靶产率因子”是指反应介质中所获得的产物浓度与shc衍生物(例如纯化的shc酶或来自表达shc酶的重组宿主细胞的萃取物)浓度之间的比率。在多个实施方案中，相对于参照shc蛋白(如seqidno.1或seqidno.2或seqidno.3或seqidno.4)，本公开的shc衍生物显示出改变的(如增加的)酶活性增加倍数(如改变的/增加的高法呢醇降龙涎香醚环化酶(hac)活性)。该活性增加为至少增加：2、3、4、6、8、10、12、14、16、18、20、25、30、35、40、45、50、55、60、65、70、75、80、85、90、95和/或100倍。核苷酸序列本公开还涉及分离的核酸分子，该核酸分子包含编码如本文所述的shc衍生物的核苷酸序列。本文所用的术语“核酸分子”应该特定地指本公开的多核苷酸，其可以为dna、cdna、基因组dna、合成dna或rna，并且可以是双链的或单链的、有义链和/或反义链。术语“核酸分子”应该特别适用于本文所用的多核苷酸，如作为全长核苷酸序列或者其片段或部分，其分别编码具有酶活性的多肽(如代谢途径的酶)、或者其片段或部分。该术语还可包括：单独的分子诸如cdna，其中对应的基因组dna基因具有内含子，因此是不同的序列；缺少旁侧基因中的至少一者的基因组片段；通过聚合酶链反应(pcr)制备并且缺少旁侧基因中的至少一者的cdna或基因组dna的片段；缺少旁侧基因中的至少一者的限制性酶切片断；编码非天然存在的蛋白质诸如融合蛋白(如his标签)、突变蛋白质或给定蛋白质的片段的dna；以及作为cdna或天然存在的核酸的简并变体的核酸。此外，其包括作为杂合基因(即编码非天然存在的融合蛋白的基因)的一部分的重组核苷酸序列。融合蛋白可给蛋白质添加一个或多个氨基酸(例如但不限于组氨酸(his))，通常是在蛋白质的n端，但也有在c端的或者融合于蛋白质区域内。这种融合蛋白或编码这种蛋白质的融合载体通常用于三个目的：(i)增加重组蛋白的产量；(ii)增加重组蛋白的溶解性；以及(iii)通过提供亲和纯化的配体而帮助对重组蛋白的纯化。术语“核酸分子”还包括适于在特定微生物宿主细胞(如大肠杆菌宿主细胞)中表达的密码子优化序列。如本文所用，术语“密码子优化”意指已通过用在细菌(如大肠杆菌)宿主细胞基因中更频繁使用的密码子取代一个密码子或多个密码子或优选大量的密码子而使其适于在原核或真核宿主细胞、尤其是细菌宿主细胞(诸如大肠杆菌宿主细胞)中表达的核酸蛋白质编码序列。就这一点而言，编码参照序列seqidno.1、2、3和/或4以及其所有的变体/衍生物的核苷酸序列可以是存在于来源中的原始序列(如分别是aacshc、zmoshc1、zmoshc2或bjpshc)或者该基因可以是针对所选择的宿主生物体(如大肠杆菌)进行了密码子优化的。可以通过体外转录制备核糖核酸(rna)分子。dna分子的区段也可视为在本公开的范围内，它们可通过例如聚合酶链反应(pcr)制备或通过用一种或多种限制性内切酶处理来生成。核酸分子的区段可称为基因(尤其是作为部分基因的那些)的dna片段。片段也可含有若干开放阅读框(orf)，可以是相同orf的重复或者是不同的orf。该术语应该具体地指编码核苷酸序列，但还应该包括非编码性的核苷酸序列(如非转录序列或非翻译序列)或编码多肽(整个多肽或部分多肽)的核苷酸序列。本文所用的基因(如用于组装、多样化或重组)可以是非编码序列，或者编码多肽的序列或蛋白质编码序列，或者其具有足以进行成功的重组事件的序列长度的部分或片段。更具体而言，所述基因最低长度为3bp、优选至少100bp、更优选至少300bp。根据上述内容将显而易见的是，提及分离的dna时，并不是指存在于例如cdna或基因组dna文库内或者基因组dna限制酶切消化物(在例如限制酶切消化反应混合物或电泳凝胶切片中)内的数百到数百万其它dna分子之中的dna。本公开的分离的核酸分子涵盖存在方式与天然状态不一样的区段。如本文所用，术语“分离的dna”可以指：(1)含有与任何天然存在的序列不同的序列的dna、不是天然存在的多核苷酸或核酸(如，通过经由人为干预将两个原本分离的序列片段人工重组(如人工操作分离的核酸片段，如通过遗传工程技术来进行)而制备，或者(2)，在具有天然存在的序列的dna(如，cdna或基因组dna)的背景下，不含有在天然存在含有所关注dna的基因的生物体基因组中处于含有所关注dna的基因旁侧的至少一个基因的dna。本文所用的术语“分离的dna”，特别是相对于核酸序列而言，也可指通过重组dna技术制备的核酸或多核苷酸，例如包含宿主细胞异源的多核苷酸的dna构建体，其任选整合进宿主细胞中。可具体地以重组分子形式制备嵌合核苷酸序列。术语“重组”应该特定地适用于多核苷酸的组装，将这样的多核苷酸或其部分连接在一起，存在或不存在重组来实现交换或基因嵌合。例如，将具有所需功能的核酸区段连接在一起以产生所需的功能组合。编码本文所述的多肽的重组基因包括以有义取向有效连接至适于表达所述多肽的一个或多个调控区的该多肽的编码序列。因为许多微生物能够从多顺反子mrna表达多种基因产物，因此可根据需要在那些微生物的单个调控区的控制下表达多种多肽。当调控区和编码序列所处位置可使得调控区可有效调控编码序列的转录或翻译时，则所述编码序列和调控区被视为有效连接。本文所用的术语“重组”(具体针对酶时)，应指由重组dna技术制备的酶，即从用编码所需酶的外源dna构建体转化的细胞产生的酶。“合成的”酶是通过化学合成制备的那些酶。可具体地作为重组分子制备嵌合酶。术语“重组dna”因此包括掺入进载体中、掺入进自主复制质粒或病毒中、或掺入进原核生物或真核生物的基因组dna中(或在同源细胞基因组中处于不同于天然染色体位置的位置处)的重组dna。在另一个方面，本公开的核酸分子有效连接至允许在原核和/或真核宿主细胞中表达的表达控制序列。如本文所用，“有效连接的”意指掺入进遗传构建体中从而表达控制序列有效控制所关注的编码序列的表达。上文提及的转录/翻译调控元件包括但不限于诱导型和非诱导型、组成型、细胞周期调控型、代谢调控型的启动子、增强子、操纵子、沉默子、阻遏子以及本领域技术人员已知的并且驱动或以别的方式调控基因表达的其它元件。这类调控元件包括但不限于指导组成型表达或允许诱导型表达的调控元件，像例如，cup-1启动子、如例如在tet-on或tet-off系统中所采用的tet阻遏子、lac系统、trp系统调控元件。举个例子，异丙基β-d-硫代半乳糖苷(iptg)在100μm至1.0mm的浓度范围内是基因表达的有效诱导物。该化合物是异乳糖的分子模拟物，异乳糖是一种乳糖代谢物，其引发乳糖操纵子的转录，因而在基因处于乳糖操纵子的控制下时其用于诱导基因表达。诱导基因表达的调控元件的另一个实例是乳糖。相似地，本公开的核酸分子可形成编码额外多肽序列(例如，充当标记或报道物的序列)的杂合基因的一部分。标记基因和报道基因的实例包括β-内酰胺酶、氯霉素乙酰转移酶(cat)、腺苷脱氨酶(ada)、氨基糖苷磷酸转移酶二氢叶酸还原酶(dhfr)、潮霉素b磷酸转移酶(hph)、胸苷激酶(tk)、lacz(编码β-半乳糖苷酶)以及黄嘌呤鸟嘌呤磷酸核糖转移酶(xgprt)。如果实践本公开相关的许多标准程序一样，技术人员将会意识到额外的有用试剂，例如可发挥标记或报道物功能的额外序列。在一些实施方案中，本公开提供编码上述wtshc或shc/hac衍生物的重组多核苷酸，其可插入载体中供表达以及任选的纯化。一种类型的载体是呈现环状双链dna环的质粒，另外的dna区段连接在其中。某些载体可控制与它们功能性连接的基因的表达。这些载体称为“表达载体”。通常适于dna重组技术的表达载体是质粒类型。典型地，表达载体包含诸如本文所述的wtshc或shc/hac变体之类的基因。在本发明描述中，术语“质粒”和“载体”可互换使用，因为质粒是最常使用的载体类型。这种载体可包含dna序列，所述dna序列包括但不限于非天然存在于宿主细胞中的dna序列、通常不转录成rna或翻译成蛋白质(“表达”)的dna序列以及期望引入进非重组宿主中的其它基因或dna序列。应当理解，通常本文所述的重组宿主的基因组通过稳定引入一种或多种重组基因而得到增强。然而，在本公开的范围内也可使用自主型或复制型的质粒或载体。此外，本公开可使用低拷贝数(如单拷贝)或高拷贝数(如本文所示例的)质粒或载体来实践。在一个优选的实施方案中，本公开的载体包含质粒、噬菌粒、噬菌体、粘粒、人工细菌或人工酵母染色体、敲除或敲入构建体、合成的核酸序列或盒，并且亚组可以以线性多核苷酸、质粒、大质粒、合成的或人工的染色体诸如植物、细菌、哺乳动物或酵母人工染色体的形式制备。优选的是引入所述载体后，在细胞内表达由所引入的多核苷酸编码的蛋白质。各式各样的基因底物可掺入质粒中。质粒通常是标准的克隆载体，如细菌多拷贝质粒。所述底物可掺入相同或不同质粒中。通常使用具有不同类型的选择标记的至少两种不同类型的质粒以允许选择含有至少两种类型的载体的细胞。通常，细菌细胞或酵母细胞可用以下核苷酸序列中的任一种或多种转化，如本领域众所周知的。对于体内重组，使用标准的转化技术将待与基因组或其它基因重组的基因用于转化宿主。在一个适当的实施方案中，将提供复制起点的dna包括在构建体中。复制起点可由技术人员适当地选择。取决于基因的性质，如果序列已经与它们自身可作复制起点起作用的基因或基因组一起存在时，则可能不需要补充的复制起点。当已将外源或异源dna引入细胞内时细菌或酵母细胞可能会被这类dna转化。转化的dna可能会或可能不会整合，即共价连接进细胞的基因组中。例如，在原核细胞以及酵母中，转化的dna可能保持在附加型元件(诸如质粒)上。对于真核细胞，稳定转染的细胞是其中转染的dna已经变得整合进染色体中从而其通过染色体复制被子代细胞遗传的细胞。该稳定性通过真核细胞能够确立由含有所述转化dna的子代细胞的群体组成的细胞系或克隆的能力来证明。一般而言，所引入的dna最初不存在于接受该dna的宿主中，但以下情况在本公开的范围内：从给定宿主分离dna区段，并随后将该dna的一个或多个额外的拷贝引入进同一宿主中，例如用以增强基因产物的产量或改变基因的表达模式。在某些情况下，引入的dna将会改变或甚至替代内源基因或dna序列，例如通过同源重组或定点诱变。合适的重组宿主包括微生物、植物细胞和植物。本公开还描述重组宿主。术语“重组宿主”，也称为“经遗传修饰的宿主细胞”或“转基因细胞”表示包含异源核酸或其基因组已通过至少一个引入的dna序列增强的宿主细胞。本公开的宿主细胞可以用上述多核苷酸或载体进行遗传工程改造。可用于本公开目的的宿主细胞包括但不限于：原核细胞诸如细菌(例如，大肠杆菌和枯草芽孢杆菌(b.subtilis))，其可用例如含有本公开的多核苷酸分子的重组噬菌体dna、质粒dna、细菌人工染色体或粘粒dna表达载体转化；简单真核细胞像酵母(例如酵母属(saccharomyces)和毕赤酵母属(pichia))，其可用例如含有本公开的多核苷酸分子的重组酵母表达载体转化。取决于用于引入本公开的多核苷酸的宿主细胞和相应载体，所述多核苷酸可以整合，例如，进入染色体或线粒体dna中，或者可以维持在染色体外，像例如附加型基因，或者可以仅暂时包含在细胞内。如本文尤其是关于遗传工程以及将一种或多种基因或组装的基因簇引入进细胞或生产细胞中所用的术语“细胞”应理解为指任何原核细胞或真核细胞。原核和真核宿主细胞二者均考虑根据本公开使用，包括细菌宿主细胞像大肠杆菌或芽孢杆菌，酵母宿主细胞诸如酿酒酵母、昆虫宿主细胞诸如草地夜蛾(spodoptorafrugiperda)，或人宿主细胞诸如hela和jurkat。特别地，所述细胞为真核细胞(优选真菌细胞、哺乳动物细胞或植物细胞)，或原核细胞。合适的真核细胞包括例如但不限于哺乳动物细胞、酵母细胞或昆虫细胞(包括sf9)、两栖动物细胞(包括黑素细胞)或蠕虫细胞，包括隐杆线虫属(caenorhabditis)(包括秀丽隐杆线虫(caenorhabditiselegans))的细胞。合适的哺乳动物细胞包括例如但不限cos细胞(包括cos-1和cos-7)、cho细胞、hek293细胞、hek293t细胞、hek293t-rextm细胞或其它可转染的真核细胞系。合适的细菌细胞包括但不限于大肠杆菌。优选地，可以使用原核生物，诸如大肠杆菌、芽孢杆菌属(bacillus)、链霉菌属(streptomyces)，或哺乳动物细胞，像hela细胞或jurkat细胞，或者植物细胞，像拟南芥属(arabidopsis)。优选地，所述细胞是曲霉属物种(aspergillussp.)或真菌细胞，优选地，其可选自以下属：酵母属、假丝酵母属(candida)、克鲁维酵母属(kluyveromyces)、汉森酵母属(hansenula)、裂殖酵母属(schizosaccharomyces)、耶氏酵母(yarrowia)、毕赤酵母属(pichia)和曲霉属(aspergillus)。优选地，大肠杆菌宿主细胞是本行业及监管部门所认可的大肠杆菌宿主细胞(包括但不限于大肠杆菌k12宿主细胞或如实施例中所展示的，大肠杆菌bl21宿主细胞)。供用于本公开的一种优选的宿主细胞是可如本文所述以重组方法制备的大肠杆菌。因而，重组宿主可以是重组大肠杆菌宿主细胞。对于大肠杆菌，存在突变体、质粒、代谢的详细计算机模型以及其它信息的文库供使用，从而允许对各种模块进行合理设计以增加产物产率。可将类似于上文针对酵母属所述的那些方法的方法用于制备重组大肠杆菌微生物。在一个实施方案中，重组大肠杆菌微生物包含编码shc基因的核苷酸序列(如例如在本文表10、11和12中的任一者或多者中所公开的，或它们的功能等价物/同源物，包括但不限于它们的变体、同源突变体、衍生物或片段)。优选地，重组大肠杆菌微生物包含如图5和21中所提供的载体构建体。在另一个优选的实施方案中，重组大肠杆菌微生物包含编码wtshc/hac和wtshc/hac衍生物基因或它们的功能等价物/同源物的核苷酸序列，所述它们的功能等价物/同源物包括但不限于如表13、表14、表15、表16、表17和/或表4a中的任一者或多者中所列出的它们的变体、同源物、突变体、衍生物或片段。用于本公开的另一优选宿主细胞是酿酒酵母(s.cerevisiae)，其为在合成生物学中广泛使用的一类生物体。因而，重组宿主可以是酿酒酵母。对于酿酒酵母，存在突变体、质粒、代谢的详细计算机模型以及其它信息的文库供使用，从而允许对各种模块进行合理设计以增加产物产率。制备重组酿酒酵母微生物的方法是已知的。细胞培养以常规方式进行。培养基含有碳源、至少一种氮源以及无机盐，并向其添加维生素。该培养基的组分可以是常规用于培养所考虑的微生物物种的组分。用于本方法的碳源包括可被重组宿主细胞代谢以利于生长和/或产生(-)-降龙涎醚的任何分子。合适碳源的实例包括但不限于蔗糖(例如，如糖蜜中所存在的)、果糖、木糖、甘油、葡萄糖、纤维素、淀粉、纤维二糖或其它含葡萄糖的聚合物。在采用酵母作为宿主的实施方案中，例如，诸如蔗糖、果糖、木糖、乙醇、甘油和葡萄糖之类的碳源是合适的。可在整个培养周期将碳源提供给宿主生物体，或者备选地，可让该生物体在存在另一种能量来源(如蛋白质)的情况下生长一段时间，然后仅在补料分批培养阶段提供碳源。重组宿主细胞微生物在本公开的方法中的适用性可通过使用众所周知的方法进行简单的测试程序来确定。例如，可将待测试的微生物在丰富培养基(如lb培养基、细菌用胰蛋白胨酵母浸膏培养基、营养培养基等)中在通常用于该微生物繁殖的ph、温度以及通气条件下繁殖。一旦选择了产生所需的生物转化产物的重组微生物(即重组宿主细胞)，则通常通过合适的表达系统和发酵由生产宿主细胞系以大规模(如通过细胞培养中的微生物生产)产生产物。在本公开的一个实施方案中，将已知成份的基本培养基如m9a用于细胞培养。m9a培养基的组分包含：14g/lkh2po4、16g/lk2hpo4、1g/l柠檬酸三钠.2h2o、7.5g/l(nh4)2so4、0.25g/lmgso4.7h2o、0.015g/lcacl2.2h2o、5g/l葡萄糖和1.25g/l酵母萃取物。在本公开的另一个实施方案中，使用富营养培养基如lb。lb培养基的组分包含：10g/l胰蛋白胨、5g/l酵母萃取物、5g/lnacl。矿质培养基和m9矿质培养基的其它实例例如公开于us6524831b2和us2003/0092143a1中。可以以分批工艺、补料分批工艺或连续工艺或它们的组合来培养重组微生物。通常，在发酵罐中在存在合适营养源(如碳源)的情况下于确定温度下培养重组微生物所需的一段时间以产生足够的酶来将高法呢醇生物转化为降龙涎醚以及产生所需量的降龙涎醚，包括(-)-降龙涎醚。可以以任何合适的方式，例如通过分批培养或补料分批培养来培养重组宿主细胞。如本文所用，术语“分批培养”是其中在培养期间既不添加也不移出培养基的培养方法。如本文所用，术语“补料分批”意指其中在培养期间添加培养基但不移出培养基的培养方法。本公开的一个实施方案提供了在细胞系统中制备降龙涎醚的方法，该方法包括在细胞系统中在合适的条件下表达wtshc或shc/hac衍生物，将高法呢醇供料给该细胞系统，利用用该细胞系统产生的shc或shc/hac衍生物使高法呢醇转化为降龙涎醚，从细胞系统收集降龙涎醚以及任选从该系统分离(-)-降龙涎醚材料。其它核苷酸序列的表达可用来增强该方法。该生物转化方法可包括在细胞系统中额外表达其它核苷酸序列。其它核苷酸序列的表达可增强制备(-)-降龙涎醚的生物转化途径。本公开的另一个实施方案是制备(-)-降龙涎醚的生物转化方法，该方法包括培养包含wtshc/hac或shc/hac衍生物基因的宿主细胞，在该宿主细胞中产生wtshc/hac或shc/hac衍生物，将高法呢醇(如eeh)供料给该宿主细胞，在适于促进高法呢醇转化为降龙涎醚的ph、温度和增溶剂条件下温育所述宿主细胞，以及收集(-)-降龙涎醚。在宿主细胞中产生wtshc/hac和/或shc/hac衍生物提供了当在合适反应条件下将高法呢醇添加至所述宿主细胞时制备(-)-降龙涎醚的方法。所实现的转化可通过将更多生物催化剂和sds添加至反应混合物来增强。可将重组宿主细胞微生物以多种方式培养以便提供合适量的表达wtshc或shc/hac衍生物的细胞供后续的生物转化步骤。因为适用于该生物转化步骤的微生物多种多样(如酵母、细菌和真菌)，所以当然要根据每种物种的具体要求调整培养条件，这些条件是众所周知的并且有文献记载的。可将培养重组宿主细胞微生物的细胞的本领域已知方法的任一种用于制备可用于本公开的后续生物转化步骤的细胞。通常，将细胞培养至特定密度(可作为光密度(od)测量)以产生足够的生物质供所述生物转化反应。所选择的培养条件不仅影响所获得的细胞(生物质)的量，而且培养条件的质量还影响生物质变成生物催化剂的情况。表达wtshc或shc/hac衍生物基因并产生wtshc或shc/hac衍生酶的重组宿主细胞微生物称为生物催化剂，其适用于生物转化反应。在一些实施方案中，生物催化剂是产生wtshc或shc/hac衍生物的重组完整细胞，或者其可以为悬浮液或固定化的形式。在其它实施方案中，生物催化剂是从产生wtshc或shc/hac衍生物的重组完整细胞制备的膜级分或液体级分(如例如上文所引用的seitz等人2012中所公开的)。产生wtshc或shc/hac衍生物的重组全细胞包括从发酵罐收集的全细胞(用于生物转化反应)或发酵罐中的细胞(则其用于一锅法反应)。产生wtshc或shc/hac衍生物的重组全细胞可包括完整的重组全细胞和/或细胞碎片。无论哪种方式，wtshc或shc/hac衍生物都以某种方式与膜(诸如细胞膜)结合，以便接受底物(如高法呢醇)和/或与底物(如高法呢醇)反应，该膜(诸如细胞膜)可以是全细胞(如重组全细胞)的一部分或构成全细胞(如重组全细胞)。wtshc或shc/hac衍生物还以是固定化形式(如与酶载体关联)，该形式允许wtshc或shc/hac衍生物与底物(如高法呢醇)反应。wtshc或shc/hac衍生物也可以以可溶形式使用。在一个实施方案中，以足够的量产生生物催化剂(生成足够的生物质)，收获并洗涤(任选保存(如冷冻或冻干保存))，然后用于生物转化步骤。在一另外的实施方案中，以足够的量产生细胞(生成足够的生物催化剂)，然后调节反应条件而无需收获和洗涤该生物催化剂供生物转化反应。该一步法(或“一锅法”)是有利的，因为其简化了工艺同时降低了成本。用于培养细胞的培养基也适合用于该生物转化反应，前提条件是调节反应条件以有利于该生物转化反应。用于培养细胞的最佳ph在6.0-7.0的范围内。用于生物转化反应的最佳ph取决于生物转化反应中所用的shc/hac酶的类型。采用技术人员众所周知的技术调节ph。如实施例9中所展示的，将“一锅法”用于将高法呢醇生物转化为(-)-降龙涎醚，转化率为100％。如实施例18中所展示的，将“一锅法”用于将高法呢醇生物转化为(-)-降龙涎醚，转化率为99％。如本文所用，本文中任何提及“高法呢醇底物转化为(-)-降龙涎醚的转化率为99％/100％”，均是指“使用wtshc/hac或shc/hac衍生酶使能够转化的高法呢醇异构体(即eeh)99％/100％转化为(-)-降龙涎醚”。虽然在本公开中术语“混合物”或“反应混合物”可与术语“培养基”互换使用(尤其是在涉及“一锅法”反应时)，应该指出的是，培养细胞以产生足够的生物质需要细胞培养基/发酵培养基，但是对于生物转化步骤不需要培养基，因为在合适的ph下反应缓冲液将足够。本公开的生物转化方法在时间、温度、ph和增溶剂的条件下进行以提供高法呢醇原料向(-)-降龙涎醚的转化。反应混合物的ph对于shc/hac衍生酶可以在4-8、优选5至6.5、更优选4.8-6.0的范围内，对于wtshc酶可以在约ph5.0至约ph7.0的范围内，并且可通过添加缓冲液至反应混合物来维持。用于该目的的示例性缓冲液是柠檬酸缓冲液。优选的温度介于约15℃至约45℃之间、优选介于约20℃至约40℃之间，但对于嗜热生物体、尤其是如果使用来自嗜热微生物的野生型酶(如wtshc/hac)的话，温度可以更高，可高达55℃。在生物转化过程期间温度可保持恒定或者可改变。本申请人已展示在生物转化反应物中包括增溶剂(如表面活性剂、去垢剂、溶解性增强剂、水混溶性有机溶剂等)可能是有用的。如本文所用，术语“表面活性剂”意指降低两种液体之间或液体与固体之间的表面张力(或界面张力)的组分。表面活性剂可充当去垢剂、润湿剂、乳化剂、发泡剂和分散剂。表面活性剂的实例包括但不限于tritonx-100、tween80、牛黄脱氧胆酸盐、牛黄脱氧胆酸钠、十二烷基硫酸钠(sds)和/或月桂基硫酸钠(sls)。虽然tritonx-100可用于部分纯化wtshc/hac或shc/hac衍生酶(以可溶形式或膜级分/悬浮液形式)，其还可用于生物转化反应(参见例如seitz(2012博士论文，如上文所引用的)中的公开内容，以及neumann和simon(1986，如上文所引用的)中的公开内容，以及jp2009060799)然而，出人意料的是，如实施例14所展示的，申请人从众多其它没那么有用的增溶剂中选择了sds并将其鉴定为特别有用的增溶剂。具体地讲，比起例如tritonx-100，依据高法呢醇转化为(-)-降龙涎醚的生物转化反应的反应速率和产率(当以4g/l和125g/l使用eeh时)，申请人将sds鉴定为明显更好的增溶剂。如实施例12中的对比数据所展示的，申请人已证明，对于至少一种shc/hac衍生酶，在反应中使用tritonx-100(以约0.005％至0.48％的浓度范围)时高法呢醇转化为(-)-降龙涎醚的最大生物转化活性仅为使用sds(以约0.07％的浓度)时所获得的活性的约20％。尽管不希望受理论束缚，将sds用于重组微生物宿主细胞可能是有利的，因为sds可能会有利地与宿主细胞膜反应以使shc酶(其为结合膜的酶)更容易被高法呢醇底物触及。此外，在反应混合物中包含适当水平的sds可改善乳液(水中的高法呢醇)的性质和/或改善高法呢醇底物向宿主细胞内的shc酶的接近而同时防止破坏(如shc(wt或shc/hac衍生物)酶的变性)。生物转化反应中所用的增溶剂(如sds)的浓度受生物质的量以及底物(eeh)浓度的影响。也就是说，在增溶剂(如sds)浓度、生物质的量以及底物(eeh)浓度之间存在一定程度的相互依赖性。举个例子，当高法呢醇底物浓度增加时，发生有效生物转化反应需要足够量的生物催化剂和增溶剂(如sds)。例如，如果增溶剂(如sds)浓度过低，则可能会观察到次优的高法呢醇转化。另一方面，例如，如果增溶剂(如sds)浓度过高，则存在生物催化剂因完整微生物细胞的破坏和/或shc/hac酶的变性/失活而受到影响的风险。根据生物质的量以及底物(eeh)浓度选择合适的sds浓度在技术人员的知识内。举个例子，有预测模型供技术人员使用来确定合适的sds、底物(eeh)和生物质的浓度。另外举个例子，实施例3证明，当使用4g/l的eeh以及达到od＝10.0(650nm)的生物催化剂时，0.010-0.075％范围内的sds是恰当的。实施例7证明，当125g/l的eeh与2倍湿重的生物质一起使用时，调节sds浓度(1.55％)是恰当的。然而，使用不同的sds/细胞比值研究eeh转化为(-)-降龙涎醚的转化百分比表明，正确选择生物催化剂、高法呢醇底物和增溶剂(如sds)的比率有利于开发稳健的生物转化反应系统，该系统展示出一定程度的对一系列sds浓度(参见例如图17)和ph范围(参见实施例15、图18)的耐受性。wtshc酶(如aacshc)的生物转化反应的温度为约45-60℃、优选55℃。wtshc酶(如aacshc)的生物转化反应的ph范围为约5.0至7.0、更优选约5.6至约6.2、甚至更优选约6.0。shc/hac衍生酶的生物转化反应的温度为约34℃至约50℃、优选约35℃。shc/hac衍生酶的生物转化反应的ph为约4.8-6.4、优选约5.2-6.0。优选地，用于生物转化反应中的增溶剂是sds。当使用约4g/l的eeh时，wtshc酶(如aacshc)的生物转化反应中所用的sds浓度在约0.010-0.075％的范围内，优选为约0.030％。当使用约4g/l的eeh时，shc/hac衍生酶的生物转化反应中所用的sds浓度在约0.0025-0.090％的范围内，优选为约0.050％。当反应物中加载有eeh浓度为约4g/l的eeh的高法呢醇时，将生物催化剂加载到反应物中至od为10.0(650nm)。当生物催化剂与eeh高法呢醇的比率为约2:1时，[sds]/[细胞]比率在约10:1-20:1的范围内、优选约15:1-18:1、优选约16:1。当高法呢醇浓度约为125g/l的eeh并且生物催化剂浓度为250g/l(对应于od约为175(650nm))时，shc变体酶的生物转化反应中的sds浓度在约1-2％的范围内、优选在约1.4-1.7％的范围内、甚至更优选约1.5％。生物催化剂与eeh高法呢醇底物的比率在约0.5:1-2:1的范围内，在一些实施方案中为2:1、优选为约1:1或0.5:1。在一些实施方案中,利用添加了高法呢醇底物的生物催化剂制备降龙涎醚。通过采用已知的手段(如蠕动泵、输注注射器等)给料来添加底物是可能的。高法呢醇是油溶性化合物并以油形式提供。鉴于生物催化剂(微生物细胞，诸如完整的重组全细胞和/或细胞碎片和/或固定化酶)存在于水相中，当将高法呢醇添加至生物转化反应混合物时，该生物转化反应可视为三相系统(包含水相、固相和油相)。即使当存在sds时也是如此。通过澄清，当可溶性wtshc或shc/hac衍生物用作生物催化剂时，这被视为两相系统。所存在的高法呢醇异构体的数量可影响反应的速率。如实施例11所展示的，shc/hac衍生酶能够从高法呢醇异构体的复杂混合物(如ee:ez:ze:zz)将e,e-高法呢醇生物转化为(-)-降龙涎醚。然而，通常观察到较低的转化率，这与以下观点一致：除了eeh之外的高法呢醇异构体可与eeh竞争对shc/hac衍生酶的靠近，从而可能充当eeh向(-)-降龙涎醚转化的竞争性抑制剂和/或还充当备选的底物。因此，优选地，高法呢醇底物包含2-4种异构体、优选两种异构体的立体异构体混合物。因此，优选地，高法呢醇底物由2-4种异构体、优选两种异构体的立体异构体混合物组成或基本上由其组成。优选地，高法呢醇底物包含ee:ez立体异构体混合物。优选地，高法呢醇底物由ee:ez立体异构体混合物组成或基本上由其组成。如实施例9所展示的，在22.5天内进行的“一锅法”发酵和生物转化反应中，观察到重量比为87:13的ee:ez100％转化。在该时间段内约10geeh发生转化。如实施例7中详细描述的，在一个优选的实施方案中，将发酵罐用于将表达shc/hac衍生基因并且产生活性shc/hac衍生酶的重组宿主细胞培养至适合在同一发酵容器中用作生物催化剂的足够生物质浓度，该生物催化剂用于将高法呢醇源转化为与副产物(ii)、(iv)和/或(iii)中的一者或多者混合的(-)-降龙涎醚，如例如图12中所公开的。可通过以下方式来分离(-)-降龙涎醚：汽提/蒸馏，或使用非水混溶性溶剂的有机溶剂萃取(以将反应产物和未反应的底物与保持在水相中的生物催化剂分离)，然后后续蒸发掉溶剂，以获得粗制反应产物，这可通过气相色谱(gc)分析来确定。汽提/蒸馏和有机溶剂萃取方法是本领域技术人员已知的。举个例子，可使用有机溶剂诸如非水混溶性溶剂(例如甲苯)从整个反应混合物萃取所得的(-)-降龙涎醚。备选地，可使用水混溶性溶剂(例如乙醇)或非水混溶性溶剂(例如甲苯)从反应混合物的固相(通过例如离心或过滤获得的)萃取所产生的(-)-降龙涎醚。举另一个例子，(-)-降龙涎醚作为晶体或以无定形形式存在于固相中，可通过过滤与其余的固相(细胞材料或其碎片)和液相分离。再举个例子，在(-)-降龙涎醚的熔点(约75℃)以上的温度下，(-)-降龙涎醚可在水相顶部形成油层，可移出该油层并收集。为了确保在移出油层后完全回收(-)-降龙涎醚，可添加有机溶剂至含有生物质的水相以便萃取生物质中或生物质上或生物质周围所含的任何残留的(-)-降龙涎醚。可将该有机层与该油层合并，然后进一步处理以分离并纯化(-)-降龙涎醚。可使(-)-降龙涎醚进一步选择性结晶以从最终的(-)-降龙涎醚产物移除副产物(ii)、(iv)和(iii)以及任何未反应的高法呢醇底物。术语“选择性结晶”是指这样的处理步骤：通过该处理步骤引起(-)-降龙涎醚从溶剂结晶，而化合物(ii)、(iii)和(iv)保持溶解在结晶溶剂中，其程度使得分离的晶体材料仅含有(-)-降龙涎醚产品，或者如果其含有任何其它化合物(ii)、(iii)或(iv)，则它们仅以嗅觉上可接受的量存在。选择性结晶步骤可使用水混溶性溶剂诸如乙醇等。使用水中的10％乙醇萃取物或通过测试晶体材料来测定最终(-)-降龙涎醚产品的嗅觉纯度。相对于(-)-降龙涎醚产品的市售参照物测试最终(-)-降龙涎醚产品的嗅觉纯度、品质及其感官特性(sensoryprofile)。还在应用研究中由专家测试该(-)-降龙涎醚材料以确定该材料是否在其感官特性方面符合规格。(-)-降龙涎醚的各种应用包括但不限于精品香料或消费品诸如织物护理产品、化妆用具、美容护理产品和清洁产品，包括其中商业使用目前可用的降龙涎醚成分的基本上所有的产品，包括但不限于：ambrox产品(芬美意公司(firmenich))、ambroxan产品(汉高公司(henkel))、ambrofix产品(奇华顿公司(givaudan))、amberlyn产品(奎斯特公司(quest))、cetaloxlaevo产品(芬美意公司)、ambermor产品(埃姆公司(aromor))和norambrenolideether产品(太平洋公司(pacific))。(-)-降龙涎醚的选择性结晶可受未反应的高法呢醇底物还有(-)-降龙涎醚与其它可检测副产物(ii)、(iii)和/或(iv)之间比率的影响。即使仅获得10％的高法呢醇底物向(-)-降龙涎醚的转化(如实施例7中使用wtshc/hac酶所展示的)，(-)-降龙涎醚的选择性结晶仍是可能的。适用于(-)-降龙涎醚的萃取和/或选择性结晶的合适水混溶性或非水混溶性有机溶剂的实例包括但不限于：脂族烃，优选具有5至8个碳原子的脂族烃，诸如戊烷、环戊烷、己烷、环己烷、庚烷、辛烷或环辛烷；卤代脂族烃，优选具有一个或两个碳原子的那些，诸如二氯甲烷、氯仿、四氯化碳、二氯乙烷或四氯乙烷；芳族烃，诸如苯、甲苯、二甲苯、氯苯或二氯苯；脂族的无环或环状的醚或醇，优选具有4至8个碳原子的那些，诸如乙醇、异丙醇、二乙醚、甲基叔丁基醚、乙基叔丁基醚、二丙醚、二异丙醚、二丁醚、四氢呋喃；或酯，诸如乙酸乙酯或乙酸正丁酯；或酮，诸如甲基异丁基酮；或二噁烷；或这些物质的混合物。尤其优选使用的溶剂是上述的庚烷、甲基叔丁基醚(也称为mtb、叔丁基甲醚、叔丁基甲基醚和tbme)、二异丙醚、四氢呋喃、乙酸乙酯和/或它们的混合物。优选地，将水混溶性溶剂诸如乙醇用于从反应混合物的固相萃取(-)-降龙涎醚。乙醇的使用是有利的，因为其易于处理，其是无毒的并且其是环境友好的。本文所用的术语“分离的”是指已从与之相伴的组分分离或纯化的生物转化产物诸如(-)-降龙涎醚。细胞系统中产生的与其在自然界中最初起源的来源不同的实体是“分离的”，因为其将必然不含天然状态下与之相伴的组分。分离或纯化的程度可通过任何恰当的方法，如气相色谱(gs)、hplc或nmr分析来测量。在一些实施方案中，将最终产物((-)-降龙涎醚)分离并纯化至均质(如纯度为至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％或89.5％或者纯度为90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或99.5％)。理想的是，所产生的(-)-降龙涎醚的量可以为约1mg/l至约20,000mg/l(20g/l)或更高，诸如约20g/l至约200g/l或100-200g/l，优选约125g/l或150g/l或约188g/l。如实施例7所展示的，使用产生shc/hac衍生酶的重组大肠杆菌宿主细胞，经过约2天在生物转化反应中产生了至少125g/l(-)-降龙涎醚。如实施例19所展示的，倘若实现充分混合，则以188g/l的eeh或更高浓度运行生物转化是可能的，因为搅拌效率看起来是该系统的唯一限制。此外，具有改善的活性的生物催化剂(如，就具有进一步改善的活性的shc变体而言，或就shc酶产量增加而言)可在使用较少生物质的情况下改善或保持生产力，就混合效率而言使用较少生物质是有利的。例如，产生了约1至约100mg/l、约30至约100mg/l、约50至约200mg/l、约100至约500mg/l、约100至约1,000mg/l、约250至约5,000mg/l、约1,000(1g/l)至约15,000mg/l(15g/l)、或约2,000(2g/l)至约10,000mg/l(10g/l)或约2,000(2g/l)至约25,000mg/l(25g/l)或约2,000(2g/l)至约25,000mg/l(25g/l)、26,000mg/l(26g/l)、27,000mg/l(27g/l)、28,000mg/l(28g/l)、29,000mg/l(29g/l)、30,000mg/l(30g/l)、40g/l、50g/l、60g/l、70g/l、80g/l、90g/l、100g/l、110g/l、120g/l、125g/l、130g/l、140g/l、150g/l、160g/l、170g/l、180g/l、190g/l或200g/l或300g/l或400g/l或500g/l的(-)-降龙涎醚。优选地，在48小时至72小时的时间周期内产生了浓度为至少100g/l的(-)-降龙涎醚。优选地，在约48小时至72小时的时间周期内产生了浓度为约150g/l的(-)-降龙涎醚。优选地，在约48小时至72小时的时间周期内产生了浓度为约200g/l的(-)-降龙涎醚。优选地，在约48小时至72小时的时间周期内产生了浓度为约250g/l的(-)-降龙涎醚。技术人员将理解，更高的累积产量滴度可通过实施连续工艺(诸如产物移出、底物供料以及生物质的添加或(部分)取代)来实现。优选地，在包含wtshc/hac或shc/hac衍生物的重组宿主细胞的存在下eeh向(-)-降龙涎醚的生物转化产生的降龙涎醚产率为5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100(单位为摩尔百分比，并且是基于所采用的eeh的摩尔数)；特别优选地，该产率介于5至100摩尔％、10至100摩尔％、20至100摩尔％、25至100摩尔％、30至100摩尔％、35至100摩尔％之间，尤其是介于40至100摩尔％、45至100摩尔％、50至100摩尔％、60至100摩尔％、70至100摩尔％之间。shc/hac酶的活性通过反应速率定义(产物的量/(产物的量+剩余原料的量))x100)，单位为摩尔百分比。优选地，在wtshc或shc/hac衍生酶的存在下eeh向(-)-降龙涎醚的生物转化产生的(-)-降龙涎醚产率为5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100(单位为摩尔百分比，并且是基于所采用的eeh的摩尔数)；特别优选地，该产率介于5至100摩尔％、10至100摩尔％、20至100摩尔％、25至100摩尔％、30至100摩尔％、35至100摩尔％之间，尤其是介于40至100摩尔％、45至100摩尔％、50至100摩尔％、60至100摩尔％、70至100摩尔％之间。在本发明的一个优选的实施方案中，在例如4、6、8、10、12、16、20、24、36或48个小时的确定时间段内测定产率和/或反应速率，在该时间段内包含编码根据本公开的wtshc或shc/hac衍生酶的核苷酸序列的重组宿主细胞将eeh转化为(-)-降龙涎醚。在一个另外的变型形式中，在例如25℃、30℃、40℃、50℃或60℃的精确确定的条件下进行反应。具体地讲，通过在35℃下进行由根据本发明的shc/hac衍生酶催化的eeh转化成(-)-降龙涎醚的反应持续24-72小时来测定产率和/或反应速率。在本发明的另一个实施方案中，包含编码shc/hac衍生物的核苷酸序列的重组宿主细胞表征为，在相同条件下与wtshc或shc/hac衍生酶相比，在高法呢醇生成(-)-降龙涎醚的反应中，其显示出2倍、3倍、4倍、5倍、6倍、7倍、8倍、9倍、10倍、11倍、12倍、13倍、14倍、15倍、16倍、17倍、18倍、19倍、20倍、21倍、22倍、23倍、24倍、25倍、26倍、27倍、28倍、29倍、30倍、31倍、32倍、33倍、34倍、35倍、36倍、37倍、38倍、39倍、40倍、41倍、42倍、43倍、44倍、45倍、46倍、47倍、48倍、49倍、50倍、51倍、52倍、53倍、54倍、55倍、56倍、57倍、58倍、59倍、60倍、61倍、62倍、63倍、64倍、65倍、66倍、67倍、68倍、69倍、70倍、71倍、72倍、73倍、74倍、75倍、76倍、77倍、78倍、79倍、80倍、81倍、82倍、83倍、84倍、85倍、86倍、87倍、88倍、89倍、90倍、91倍、92倍、93倍、94倍、95倍、96倍、97倍、98倍、99倍、100倍、200倍、500倍、1000倍或更高的产率和/或反应速率。这里，术语“条件”涉及反应条件，诸如底物浓度、酶浓度、反应期和/或温度。成功开发一种在包含编码wt/参照shc或shc/hac衍生物的核苷酸序列的大肠杆菌重组菌株中由高法呢醇制备(-)-降龙涎醚的生物转化工艺可提供低成本且在工业上经济的(-)-降龙涎醚生产工艺。如实施例7所展示的，在与经优化的shc/hac衍生物温育48小时后，本公开提供了e,e-高法呢醇(125g/l)向(-)-降龙涎醚的100％转化，与wtaacshc酶相比，当使用aacshc衍生物时产率提高8倍(见图11)。wt参照shc/hac或本文所述的shc/hac衍生物多肽的功能同源物也适合用于在重组宿主中制备降龙涎醚。因而，重组宿主可包含一种或多种编码上述多肽的功能同源物的异源核酸和/或编码如上所述的shc/hac衍生酶的异源核酸。功能同系物是与参照多肽具有序列相似性，以及执行参照多肽的一种或多种生化功能或生理功能的多肽。功能同系物和参照多肽可能是天然存在的多肽，并且序列相似性可能是由于趋同进化或趋异进化事件造成。照此，功能同系物有时候在文献中称为同系物，或直系同源物或旁系同源物。天然存在的功能同系物的变体，诸如由野生型编码序列的突变体编码的多肽，可能它们自身是功能同系物。功能同系物还可以经由多肽的编码序列的定点诱变，或通过组合来自不同天然存在的多肽的编码序列的结构域(“结构域交换”)来生成。用于修饰编码本文所述的功能同系物的基因的技术是已知的，并且尤其包括定向进化技术、定点诱变技术和随机诱变技术，并且可用于增加多肽的比活性、改变底物特异性、改变表达水平、改变亚细胞定位或以所需方式修饰多肽:多肽相互作用。这种经修饰的多肽被视为功能同系物。术语“功能同系物”有时候适用于编码在功能上同源的多肽的核酸。功能同系物可通过对核苷酸和多肽序列比对进行分析而鉴定。例如，对核苷酸或多肽序列的数据库进行查询可鉴定编码shc衍生物多肽等的核酸序列的同系物。还可将杂交用于鉴定功能同系物和/或用作两条核酸序列之间的同源性的量度。编码任何本文所公开的蛋白质的核酸序列或其部分可根据标准的杂交技术用作杂交探针。探针杂交至来自测试来源(如哺乳动物细胞)的dna或rna是该测试来源中存在相关dna或rna的指示。杂交条件是本领域技术人员已知的并且可见于currentprotocolsinmolecularbiology,johnwiley&sons,n.y.,6.3.1-6.3.6,1991。中等杂交条件定义为相当于在30℃下于2x氯化钠/柠檬酸钠(ssc)中杂交，然后在50℃下于1xssc、0.1％的sds中洗涤。高严格条件定义为相当于在45℃下于6x氯化钠/柠檬酸钠(ssc)中杂交，然后在65℃下于0.2xssc、0.1％的sds中洗涤。鉴定功能同系物的序列分析也可涉及使用一相关氨基酸序列作为参照序列对非冗余数据库进行blast、交互blast或psi-blast分析。在某些情况下，氨基酸序列从核苷酸序列推导而来。数据库中具有大于40％序列同一性的那些多肽是用于进一步评价在shc/hac生物转化反应中使用的适用性的候选者。氨基酸序列相似性使得能进行保守氨基酸取代，诸如将一个疏水残基取代为另一个疏水残基或者将一个极性残基取代为另一个极性残基。如果需要，可对这些候选者进行人工检测以便缩窄待进一步评价的候选者的数目。通过选择看起来具有例如保守功能结构域的那些候选者来进行人工检测。通常，表现出至少30％氨基酸序列同一性的多肽可用于鉴定保守区。相关多肽的保守区表现出至少30％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％、50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％的氨基酸序列同一性。在一些实施方案中，保守区表现出至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、至少81％、至少82％、至少83％、至少84％、至少85％、至少86％、至少87％、至少88％、至少89％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％的氨基酸序列同一性。可如上文和下文所述测定序列同一性。所产生的wtshc和/或shc/hac衍生物是基于氨基酸seqidno.1或seqidno.2或seqidno.3或seqidno.4或它们的变体、同源物、突变体、衍生物或片段。所产生的shc是基于与seqidno.1或seqidno.2或seqidno.3或seqidno.4具有至少30％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％、50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、至少81％、至少82％、至少83％、至少84％、至少85％、至少86％、至少87％、至少88％、至少89％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列。此外，所产生的参照shc是基于由大肠杆菌产生的氨基酸序列。关于基因的核苷酸序列的“同一性百分比(％)”定义为：在比对序列和引入缺口(必要时)以实现最大的序列同一性百分比、并且不将任何保守取代视作序列同一性的一部分之后，候选dna序列中与所述dna序列中的核苷酸相同的核苷酸的百分比。为了测定核苷酸序列同一性百分比的比对可以各种方式来实现，这些方式在本领域技术人员的范围内，例如，使用可公开获得的计算机软件。本领域内的技术人员可确定用于测量比对的恰当参数，包括在进行比较的序列的整个长度上实现最大比对所需的任何算法。术语“多肽”和“蛋白质”在本文中可互换使用，意指任何氨基酸的肽键连接的链，而不管长度或翻译后修饰。如本文所用的，术语“衍生物”包括但不限于变体。术语“衍生物”和“变体”在本文中可互换使用。如本文所用，术语“变体”应该理解为这样一种多肽：与衍生该多肽的多肽相比，在氨基酸序列中有一个或多个改变。衍生出变体的多肽也称为亲本多肽或参照多肽。通常以人工方式构建变体，优选通过基因技术手段。通常，衍生出变体的多肽是野生型蛋白质或野生型蛋白质结构域。然而，可用于本公开的变体也可以衍生自亲本多肽的同系物、直系同源物或旁系同源物，或者源于人工构建的变体，前提条件是该变体表现出亲本多肽的至少一种生物活性。氨基酸序列中的改变可以是氨基酸交换、插入、缺失、n端截短或c端截短、或这些改变的任何组合，这些改变可在一个或数个位点发生。在优选的实施方案中，可用于本公开的变体在氨基酸序列中表现出总数高达200(高达1、2、3、4、5、6、7、8、9、10、15、20、25、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190或200)个改变(即交换、插入、缺失、n端截短和/或c端截短)。氨基酸交换可以是保守性的和/或非保守性的。在优选的实施方案中，可用于本公开的变体与衍生该变体的蛋白质或结构域相差最多1、2、3、4、5、6、7、8、9、10、15、20、25、30、35、40、45、50、55、60、65、70、75、80、85、90、95或100个氨基酸交换，优选保守氨基酸改变。变体可额外地或备选地包含氨基酸缺失，其可以是n端截短、c端截短或内部缺失或这些情况的任何组合。包含n端截短、c端截短和/或内部缺失的这些变体在本申请的上下文中称为“缺失变体”或“片段”。术语“缺失变体”和“片段”在本文中可互换使用。缺失变体可以是天然存在的(如剪接变体)或其可以人工构建，优选通过基因技术手段。通常，衍生缺失变体的蛋白质或蛋白质结构域是野生型蛋白质。然而，本公开的缺失变体也可以衍生自亲本多肽的同系物、直系同源物或旁系同源物，或者源于人工构建的变体，前提条件是该缺失变体表现出亲本多肽的至少一种生物活性。优选地，与亲本多肽相比，缺失变体(或片段)在其n端和/或在其c端和/或在内部具有最多1、2、3、4、5、6、7、8、9、10、15、20、25、30、35、40、45、50、55、60、65、70、75、80、85、90、95或100个氨基酸的缺失。作为另外一种选择或额外地，本文所用的“变体”可表征为与衍生该变体的亲本多肽具有一定程度的序列同一性。本公开的wt/参照shc/hac或shc/hac衍生物的变体可以与各自的参照多肽或与各自的参照多核苷酸具有至少40％、41％、42％、43％、44％、45％、46％、47％、48％、49％、50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、至少81％、至少82％、至少83％、至少84％、至少85％、至少86％、至少87％、至少88％、至少89％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％的序列同一性。表述“至少30％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％、50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、至少81％、至少82％、至少83％、至少84％、至少85％、至少86％、至少87％、至少88％、至少89％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％序列同一性”就多肽和多核苷酸序列比较而言在整篇说明书中使用。属于本文所公开的任何酶的家族的多核苷酸或蛋白质可分别根据它们与相关基因或蛋白质的相似性来鉴别。例如，该鉴别可以是基于序列同一性。在某些优选的实施方案中，本公开描述了分离的核酸分子，其与以下核酸分子具有至少30％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％、50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、至少81％、至少82％、至少83％、至少84％、至少85％、至少86％、至少87％、至少88％、至少89％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％的同一性：(a)编码seqidno.5-163(参见本文提供的表14-17和表4a)的多肽的核酸分子，(b)seqidno.6-168、169、170、172、174和176(参见本文所提供的表14-17和表4a)的核苷酸序列，以及(c)包括seqidno.6-168、169、170、172、174和176(参见本文所提供的表14-17和表4a)的至少30(如至少30、40、50、60、80、100、125、150、175、200、250、300、400、500、600、700、800、850、900、950、1000或1010)个核苷酸的区段的核酸分子。优选地，所考虑的多肽与参照多肽在20、30、40、45、50、60、70、80、90、100或更多个氨基酸的连续链段上表现出所指示的序列同一性。优选地，所考虑的多核苷酸与参照多核苷酸在60、90、120、135、150、180、210、240、270、300或更多个核苷酸的连续链段上表现出所指示的序列同一性。在其中对两条序列进行比较并且在比较中要计算与其的序列同一性的参照序列未规定的情况中，如果未另外明确指明的话，则序列同一性应参照待比较的两条序列中的较长者进行计算。如果指明了参照序列，则如果未另外明确指明的话，基于seqidno.1、2、3和/或4指示的参照序列的全长测定序列同一性。例如，由130个氨基酸组成的肽序列与具有631个氨基酸残基的参照shc的全长氨基酸相比较，可展现出20.6％的最大序列同一性百分比(130/631x100)，而具有300个氨基酸长度的序列可展现出47.5％的最大序列同一性百分比(300/631x100)。核苷酸序列和氨基酸序列的相似性(即序列同一性百分比)可通过序列比对测定。这种比对可用数种本领域已知的算法，优选用karlin和altschul(karlin&altschul(1993)proc.natl.acad.sci.usa90:5873-5877)的数学算法、用hmmalign(hmmer软件包，http://hmmer.wustl.edu/)或用clustal算法(thompson,j.d.,higgins,d.g.&gibson,t.j.(1994)nucleicacidsres.22,4673-80)(在例如http://www.ebi.ac.uk/tools/clustalw/上或在http://www.ebi.ac.uk/tools/clustalw2/index.html上或在http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl？page＝/npsa/npsa_clustalw.html上可供使用)或用gap程序(爱荷华大学的数学算法)(如与wtshc的序列比对中所用的，如本文表18中所提供的)或myers和miller(1989-cabios4:11-17)的数学算法(如本文所提供的表19中的wtshc序列比对中所公开的并且如其中所用的)来进行。所用的优选参数是如它们在http://www.ebi.ac.uk/tools/clustalw/或http://www.ebi.ac.uk/tools/clustalw2/index.html上设定的默认参数。序列同一性(序列匹配)等级可使用例如blast、blat或blastz(或blastx)计算。类似的算法并入了altschul等人(1990)j.mol.biol.215,403-410的blastn和blastp程序中。blast多核苷酸搜索用blastn程序，得分(score)＝100，字长(wordlength)＝12来进行，以获得与编码相关蛋白质的那些核酸同源的多核苷酸序列。blast蛋白质搜索用blastp程序，得分＝50，字长＝3来进行，以获得与srko多肽同源的氨基酸序列。为了获得带缺口的比对以达到比较目的，如altschul等人(1997)nucleicacidsres.25,3389-3402中所述使用gappedblast。当使用blast和gappedblast程序时，使用各程序的默认参数。序列匹配分析可通过确立的同源性作图技术如shuffle-lagan(brudnom.,bioinformatics2003b,19，增刊1:154-162)或马尔可夫随机场来补充。当在本申请中提及序列同一性百分比时，如果未另外明确指明，则这些百分比是相对于较长序列的全长计算的。感官方面根据本公开的高法呢醇向(-)-降龙涎醚的生物转化产生(-)-降龙涎醚作为优势化合物，但是也可能产生除了(-)-降龙涎醚之外的化合物，这些化合物可能会或可能不会给该生物转化混合物赋予令人愉悦的嗅觉香型，从而可能以正面或负面的方式促成(-)-降龙涎醚终产物的感官品质。因此，感官分析使用由经训练的专家(香水师)进行的已确立的感官测试进行，从而这种测试可帮助确定化学相关的靶产物相对于参照产品是否也是嗅觉相关的终产物。如实施例22中的感官分析所展示的，从(-)-降龙涎醚移除一种或多种副产物化合物可改善剩余化合物((-)-降龙涎醚)的气味，即使所移除的化合物实际上本身是无臭化合物。也就是说，在缺少化合物ii、iii和iv的情况下观察到(-)-降龙涎醚气味增强。本发明的方面1.一种制备(-)-降龙涎醚或包含(-)-降龙涎醚的混合物的方法，其中(3e,7e)-高法呢醇(eeh)或包含eeh的立体异构体混合物被酶促转化为(-)-降龙涎醚或包含(-)-降龙涎醚的混合物，其中所述酶促转化使用shc/hac酶在适于产生(-)-降龙涎醚的反应条件下进行，并且其中包含eeh的立体异构体混合物基本上由选自以下的高法呢醇异构体组成：[(3e,7e)和[(3z,7e)]和/或[(3e,7e)和(3e,7z)]和/或[(3z,7e)、(3e,7e)和(3e,7z)]，它们也分别被命名为[ee:ez]、[ee:ze]和[ee:ez:ze]。2.一种制备(-)-降龙涎醚或包含(-)-降龙涎醚的混合物的方法，其中(3e,7e)-高法呢醇(eeh)或包含eeh的立体异构体混合物经酶促转化而得到(-)-降龙涎醚或包含(-)-降龙涎醚的混合物，其中所述酶促转化使用shc/hac酶在适于产生(-)-降龙涎醚的反应条件下进行，并且其中如果所述反应在存在增溶剂的情况下进行，则不将tritonx-100或牛黄脱氧胆酸盐与野生型shc/hac酶结合使用。3.一种制备(-)-降龙涎醚或包含(-)-降龙涎醚的混合物的方法，其中(3e,7e)-高法呢醇(eeh)或包含eeh的立体异构体混合物被酶促转化为(-)-降龙涎醚或包含(-)-降龙涎醚的混合物，其中所述酶促转化使用shc/hac酶在适于产生(-)-降龙涎醚的反应条件下进行，并且其中包含eeh的立体异构体混合物基本上由选自以下的高法呢醇异构体组成：[(3e,7e)和[(3z,7e)]和/或[(3e,7e)和(3e,7z)]和/或[(3z,7e)、(3e,7e)和(3e,7z)]，它们也分别被命名为[ee:ez]、[ee:ze]和[ee:ez:ze]，并且其中所述反应在包含水相、固相和油相的三相系统中发生。4.根据段落1或段落2或段落3的方法，其中所述方法使用选自seqidno.1、seqidno.2、seqidno.3、seqidno.4的shc/hac酶多肽序列或选自表1、表5、表2、表6、表3、表7、表4、表8或表13、表14，或选自seqidno.5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、171、173、175、177和/或178的shc/hac衍生物或与seqidno.1、seqidno.2、seqidno.3或seqidno.4具有至少30％同一性、至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的序列进行。5.段落1-4中任一项的方法，其中所述方法使用产生shc/hac酶的重组宿主细胞。6.根据段落4或段落5的方法，其中所述编码shc/hac酶的核苷酸序列选自seqidno.165、166、167、168、169或seqidno.6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40和/或170、172、174和/或176。7.根据段落1-6中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化在约4-8的ph下于30℃至60℃的温度下发生。8.根据段落1-7中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于野生型shc/hac或shc/hac衍生酶的反应条件中的一者或多者，优选在5.0至6.2的ph范围下、优选在35℃的温度下进行。9.根据段落3-8中任一项的方法，其中当生物催化剂与eeh的比率约为2:1时，sds/细胞比率在10:1至20:1的范围内，优选为16:1。10.根据段落3-9中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5-2:1的范围内，优选约1:1或0.5:1。11.根据段落3-10中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。12.根据段落2的方法，其中所述高法呢醇底物包含一种或多种高法呢醇立体异构体。13.根据段落12的方法，其中所述高法呢醇底物包含两种高法呢醇立体异构体或基本上由两种高法呢醇立体异构体组成。14.根据段落13的方法，其中所述高法呢醇底物包含ee:ez立体异构体或基本上由ee:ez立体异构体组成。15.根据段落14中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:2970:30；69:31；68:32；67:33；66:34；65:35；64:36；63:37；62:38；61:39；以及60:40。16.根据段落15的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比选自：ee:ez92:8；ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。17.根据段落15或段落16的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比为80:20。18.根据段落1-17中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。19.根据段落1-18中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述生物转化反应混合物分离(-)-降龙涎醚。20.根据段落1-19中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤从所述生物转化反应混合物的固相分离(-)-降龙涎醚。21.根据段落19或段落20的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。22.根据段落21的方法，其中使用乙醇或甲苯从所述反应混合物分离(-)-降龙涎醚。23.根据段落19-22中任一项的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。24.根据段落23的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。25.根据段落1-24中任一项的方法，其中产生了浓度范围为约125-200g/l的(-)-降龙涎醚。26.通过根据段落1-25中任一项的方法能够获得的(-)-降龙涎醚，其中所述(-)-降龙涎醚具有约0.1至约0.5ng/l的嗅觉阈值。27.段落26的(-)-降龙涎醚，其为固体形式，优选无定形形式或晶体形式。28.一种制备含有(-)-降龙涎醚的产品的方法，包括将段落26或段落27中任一项的(-)-降龙涎醚掺入所述产品中。29.段落28的方法，其中所述产品是香料产品、化妆品、清洁产品、洗涤剂产品或皂产品。30.一种香料或化妆品或消费者护理产品，其中包含段落26或段落27中任一项的(-)-降龙涎醚。31.一种香料或化妆品或消费者护理组合物，其中包含段落26或段落27的(-)-降龙涎醚和一种或多种另外的组分。32.段落26或段落27的(-)-降龙涎醚的用途，其用作香料或化妆品或消费品诸如织物护理、化妆用具、美容护理和/或清洁产品的一部分。33.用于在香料组合物中或为香料组合物增加、增强或赋予芳香的方法，包括使所述芳香组合物与芳香增加或增强产品混合的步骤，所述芳香增加或增强产品根据包括以下步骤的方法制备：(a)制备反应混合物，所述反应混合物包含与副产物化合物(ii)、(iii)或(iv)中一种或多种相混合的(-)-降龙涎醚。(b)萃取与副产物化合物(ii)、(iii)或(iv)中的一者或多者相混合的(-)-降龙涎醚；以及(c)从所述萃取混合物中选择性结晶(-)-降龙涎醚；其中通过使用shc/hac酶在适于产生(-)-降龙涎醚的反应条件下酶促转化(3e,7e)-高法呢醇(eeh)或包含eeh的立体异构体混合物来制备(-)-降龙涎醚，并且其中所述包含eeh的立体异构体混合物基本上由选自以下的高法呢醇异构体组成：[(3e,7e)和[(3z,7e)]和/或[(3e,7e)和(3e,7z)]和/或[(3z,7e)、(3e,7e)和(3e,7z)]，它们也分别命名为[ee:ez]、[ee:ze]和[ee:ez:ze]。34.段落33的方法，其中所述反应在包含水相、固相和油相的三相系统中发生。35.根据段落33或段落34的方法，其中所述方法使用选自seqidno.1、seqidno.2、seqidno.3、seqidno.4的shc/hac酶多肽序列或选自表1、表5、表2、表6、表3、表7、表4、表8或表14，或选自seqidno.5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、171、173、175和/或177的shc/hac衍生物或与seqidno.1、seqidno.2、seqidno.3或seqidno.4具有至少30％同一性、至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的序列进行。36.段落33-35中任一项的方法，其中所述方法使用产生shc/hac酶的重组宿主细胞。37.根据段落35或段落36的方法，其中所述编码shc/hac酶的核苷酸序列选自seqidno.165、166、167、168、169或seqidno.6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40和/或170、172、174和/或176。38.根据段落33-37中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化在约4-8的ph下于30℃至60℃的温度下发生。39.根据段落33-38中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于野生型shc/hac或shc/hac衍生酶的反应条件中的一者或多者，优选在5.0至6.2的ph范围下、优选在35℃的温度下发生。40.根据段落34-39中任一项的方法，其中当生物催化剂与eeh的比率约为2:1时，sds/细胞比率在10:1至20:1的范围内，优选为16:1。41.根据段落34-40中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5-2:1的范围内，优选约1:1或0.5:1。42.根据段落34-41中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。43.根据段落33-42中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:2970:30；69:31；68:32；67:33；66:34；65:35；64:36；63:37；62:38；61:39；以及60:40。44.根据段落43的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比选自：ee:ez92:08；ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。45.根据段落43或段落44的方法，其中所述高法呢醇包含ee:ez立体异构体混合物或基本上由ee:ez立体异构体混合物组成，所述ee:ez立体异构体混合物的重量比为80:20。46.根据段落33-45中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。47.根据段落33-46中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述生物转化反应混合物分离(-)-降龙涎醚。48.根据段落33-47中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤从所述生物转化反应混合物的固相分离(-)-降龙涎醚。49.根据段落47或段落48的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。50.根据段落49的方法，其中使用乙醇或甲苯从所述反应混合物分离(-)-降龙涎醚。51.根据段落47-49中任一项的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。52.根据段落51的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。53.根据段落33-52中任一项的方法，其中产生了浓度范围为约125-200g/l的(-)-降龙涎醚。54.根据段落33-53中任一项的方法，其中所述(-)-降龙涎醚具有约0.1至约0.5ng/l的嗅觉阈值。本发明的另外的方面1.一种角鲨烯何帕烯环化酶(shc)/高法呢醇降龙涎香醚环化酶(hac)衍生物，所述衍生物包含相对于seqidno.1具有独立选自取代、缺失或插入的1-50个突变的氨基酸序列。2.根据段落1的shc/hac衍生物，其中所述shc衍生物包含相对于seqidno.1具有1至40个突变、1-30个突变、1-20个突变、1-10个突变或1-6个突变的氨基酸序列。3.根据段落1或段落2的shc/hac衍生物，其中所述shc/hac衍生物包含相对于seqidno.1具有至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的氨基酸序列。4.根据段落3的shc/hac衍生物，其中所述shc变体包含与seqidno.1具有至少95％同一性的氨基酸序列。5.一种shc/hac衍生物，其相对于seqidno.1而言包含独立选自取代、缺失或插入的1-10个突变，其中除了shc活性位点突变之外的所述一个或多个突变位于shc酶的结构域2中(图19和/或20)。6.根据段落1-5中任一项的shc/hac衍生物，其中相对于seqidno.1的所述一个或多个突变选自表1，其中如果仅选择一个突变，则其不是f601y。7.段落6的shc/hac衍生物，其中至少2、3、4、5、6、7、8、9或10个突变选自表1或表5。8.段落2的shc/hac衍生物，所述衍生物包含相对于seqidno.1而言具有最多6个突变并且包含与f129l和/或i432t中的至少任一者或多者组合的至少取代f601y或m132r的氨基酸序列。9.段落7的shc/hac衍生物，所述衍生物包含相对于seqidno.1而言具有最多8个氨基酸改变并且相对于seqidno.1在选自位置77、129、132、192、224、432、579、601和605的位置中包含一个或不止一个氨基酸改变的氨基酸序列，其中相对于seqidno.1而言所述shc/hac衍生物具有增加的hac酶促活性。10.段落9的shc/hac衍生物，所述衍生物相对于seqidno.1而言包含一个或多个取代，所述取代选自：t77a、f129l、m132r、i92v、a224v、i432t、q579h、f601y和/或f605w。11.根据段落10的shc/hac衍生物，所述衍生物包含f601y。12.根据段落10的shc/hac衍生物，所述衍生物包含f129l。13.根据段落10的shc/hac衍生物，所述衍生物包含f601y和f129l。14.根据段落10的shc/hac衍生物，所述衍生物包含m132r和i432t。15.根据段落14的shc/hac衍生物，所述衍生物还包含氨基酸取代a224v。16.根据段落14的shc/hac衍生物，所述衍生物还包含f601y。17.根据段落14的shc/hac衍生物，所述衍生物还包含f129l。18.根据段落17的shc/hac衍生物，所述衍生物还包含f601y。19.根据段落11的shc/hac衍生物，所述衍生物还包含q579h。20.根据段落10的shc/hac衍生物，所述衍生物包含t77a、i92v和f129l。21.根据前述段落任一项的shc/hac衍生物，所述衍生物具有选自seqidno.5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39和/或171的氨基酸序列。22.一种分离的核苷酸序列，所述核苷酸序列编码根据段落1-21中任一项的shc衍生物。23.根据段落22的分离的核苷酸序列，其中所述核苷酸序列选自seqidno.6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40和/或170。24.一种构建体，所述构建体包含段落22或段落23的核苷酸序列。25.根据段落24的构建体，所述构建体包含与段落22或23的核苷酸序列功能性连接的启动子。26.段落25的构建体，其中所述启动子是诱导型启动子或组成型启动子。27.一种载体，所述载体包含根据段落24-26中任一项的构建体。28.段落27的载体，其中所述载体是质粒。29.根据段落28的载体，所述载体能够在选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞中指导表达。30.段落24-26中任一项的构建体或根据段落27-29中任一项的载体，其中所述构建体或所述载体能够整合进选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞的基因组中。31.一种重组宿主细胞，所述重组宿主细胞包含根据段落22或23的核苷酸序列或根据段落24-26或30中任一项的构建体或根据段落27-30中任一项的载体。32.根据段落31的重组宿主细胞，其中所述宿主细胞选自原核宿主细胞，所述原核宿主细胞由埃希杆菌属(escherichia)、链霉菌属(streptomyces)、芽孢杆菌属(bacillus)、假单胞菌属(pseudomonas)、乳杆菌属(lactobacillus)和乳球菌属(lactococcus)的细菌组成。33.段落32的重组宿主细胞，其中所述宿主细胞是大肠杆菌宿主细胞。34.段落33的重组宿主细胞，其中所述宿主细胞过表达编码所述shc/hac衍生物的基因。35.一种制备根据段落1-21中任一项的shc/hac衍生物的方法，所述方法包括在允许产生所述shc/hac衍生酶的条件下培养一种或多种根据段落31-34中任一项的重组宿主细胞的步骤。36.段落35的方法，其中所述细胞培养在适合生物催化剂产生的条件下发生。37.一种制备(-)-降龙涎醚的方法，所述方法包括使用根据段落31-34中任一项的重组宿主细胞或通过使用包含编码wtshc/hac的seqidno.169或seqidno.165的重组宿主细胞将高法呢醇转化为(-)-降龙涎醚，其中如果使用wtshc/hac，则高法呢醇向(-)-降龙涎醚的生物转化用除了tritonx-100或牛黄脱氧胆酸盐的增溶剂进行。38.根据段落37的方法，其中高法呢醇向(-)-降龙涎醚的转化是在适用于wtshc/hac或shc/hac衍生酶的生物转化反应条件下进行。39.根据段落37或38的方法，其中高法呢醇向(-)-降龙涎醚的转化在适用于wtshc/hac或shc/hac衍生酶的ph、温度、增溶剂浓度下发生。40.根据段落39的方法，其中高法呢醇向(-)-降龙涎醚的转化在范围为30℃至60℃的温度下、在范围为约4-8的ph下并且对于wtshc/hac酶在存在除了tritonx-100或牛黄脱氧胆酸盐之外的增溶剂的情况下进行。41.根据段落37-40中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于wtshc/hac或shc/hac衍生酶的反应条件中的一者或多者进行。42.根据段落37-41中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5:1至2:1的范围内，优选约1:1或0.5:1。43.根据段落37-42中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。44.根据段落37-43中任一项的方法，其中所述高法呢醇底物包含一种或多种高法呢醇立体异构体。45.根据段落44的方法，其中所述高法呢醇底物包含两种高法呢醇立体异构体。46.根据段落45的方法，其中所述高法呢醇底物包含ee:ez立体异构体。47.根据段落44-46中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29以及70:30。48.段落47的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。49.段落35或36的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比为80:20。50.段落37-49中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。51.根据段落37-50中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤从所述生物转化反应混合物分离(-)-降龙涎醚，或者通过过滤直接从生物转化反应混合物分离(-)-降龙涎醚晶体。52.根据段落51的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。53.段落52的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。54.段落52或53的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。55.通过段落51-54中任一项的方法能够获得的(-)-降龙涎醚。56.段落55的(-)-降龙涎醚，其为固体形式，优选为无定形形式或晶体形式。57.一种制备含有(-)-降龙涎醚的产品的方法，所述方法包括将段落55或56的(-)-降龙涎醚掺入所述产品，优选香料产品、美容产品、清洁产品、洗涤剂产品或皂产品。58.一种香料或化妆品或消费者护理产品，其中包含段落55或56的(-)-降龙涎醚。59.一种香料或化妆品或消费者护理组合物，其中包含段落55或56的(-)-降龙涎醚和一种或多种另外的组分。60.段落55或56的(-)-降龙涎醚的用途，其用作香料或化妆品或消费品诸如织物护理、化妆用具、美容护理和/或清洁产品的一部分。61.根据段落1-21中任一项的shc/hac衍生酶、根据段落22或23的核苷酸序列、根据段落24-26或30中任一项的构建体、根据段落27-30中任一项的载体或根据段落31-34中任一项的重组宿主细胞或表达wtshc/hac酶的重组宿主细胞的用途，用于将高法呢醇生物转化为(-)-降龙涎醚，其中所述wtshc/hac酶与除了tritonx-100以外的增溶剂一起用于所述生物转化反应。62.一种制备(-)-降龙涎醚或(-)-降龙涎醚立体异构体混合物的方法，其中(3e,7e)-高法呢醇或(3e,7e)-高法呢醇立体异构体混合物经酶促转化而得到(-)-降龙涎醚或(-)-降龙涎醚立体异构体混合物，其中所述酶促转化使用shc/hac酶在适于产生(-)-降龙涎醚的反应条件下进行，并且其中如果所述反应在存在增溶剂的情况下进行，则不将tritonx-100与wtshc/hac酶结合使用。63.根据段落62的方法，其中所述方法使用选自aacshc(seqidno.1)、zmoshc1(seqidno.2)、zmoshc2(seqidno.3)、bjpshc(seqidno.4)的shc/hac、选自表1、表5、表2、表6、表3、表7、表4和/或表8的shc/hac衍生物或相对于seqidno.1、seqidno.2、seqidno.3和/或seqidno.4具有至少30％同一性、至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的序列进行。64.根据段落63的方法，其中高法呢醇向(-)-降龙涎醚的转化在范围为30℃至60℃的温度下、在范围为4-8的ph下并且对于wtshc在存在除了tritonx-100的增溶剂的情况下进行。65.根据段落64的方法，其中使用表24或表24a中所列出的用于wtshc/hac或每种shc/hac衍生物的反应条件。66.根据段落62-65中任一项的方法，其中所述方法包括(a)在e,e-高法呢醇转化为(-)-降龙涎醚之前，在允许表达wtshc或shc/hac衍生多肽的条件下培养表达wtshc或shc衍生酶的一个或多种重组宿主细胞。67.根据段落66的方法，其中所述培养步骤和后续的转化步骤在同一反应容器中于不同反应条件下进行。68.根据段落67的方法，其中所述培养步骤是在约6至约7的ph范围下进行，并且高法呢醇转化为(-)-降龙涎醚的步骤是在约4.8-5.5的ph范围下进行。69.根据段落62-68中任一项的方法，其中所述高法呢醇底物包含ee:ez立体异构体。70.根据段落69的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。71.段落70的方法，其中所述高法呢醇包含重量比为80:20的ee:ez。72.段落62-71中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。73.段落62-72中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述反应混合物分离(-)-降龙涎醚。74.根据段落73的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。75.段落74的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。76.段落74或75的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。77.通过段落72-76中任一项的方法能够获得的(-)-降龙涎醚。78.段落26的(-)-降龙涎醚，其为固体形式，优选为无定形形式或晶体形式。79.一种制备产品的方法，所述方法包括将段落77或78的(-)-降龙涎醚掺入所述产品中。80.段落79的方法，其中所述产品是香料产品、化妆品、清洁产品、洗涤剂产品或皂产品。81.一种香料或化妆品或消费者护理产品，其中包含段落77或78的(-)-降龙涎醚。82.一种香料或化妆品或消费者护理组合物，其中包含段落77或78的(-)-降龙涎醚和另外的组分。83.段落77或78的(-)-降龙涎醚的用途，其用作香料或化妆品、消费者护理产品的一部分。本发明的另外的方面(zmoshc1)1.一种角鲨烯何帕烯环化酶(shc)/高法呢醇降龙涎香醚环化酶(hac)衍生物，所述衍生物包含相对于seqidno.2具有独立选自取代、缺失或插入的1-50个突变的氨基酸序列。2.根据段落1的shc/hac衍生物，其中所述shc衍生物包含相对于seqidno.2具有1至40个突变、1-30个突变、1-20个突变、1-10个突变或1-6个突变的氨基酸序列。3.根据段落1或段落2的shc/hac衍生物，其中所述shc/hac衍生物包含相对于seqidno.2具有至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的氨基酸序列。4.根据段落3的shc/hac衍生物，其中所述shc变体包含与seqidno.2具有至少95％同一性的氨基酸序列。5.一种shc/hac衍生物，其相对于seqidno.2而言包含独立选自取代、缺失或插入的1-10个突变，其中除了shc活性位点突变之外的所述一个或多个突变位于shc酶的结构域2中(图19和/或20)。6.根据段落1-5中任一项的shc/hac衍生物，其中相对于seqidno.2的所述一个或多个突变选自表2，其中如果仅选择一个突变，则其不是f668y。7.段落6的shc/hac衍生物，其中至少2、3、4、5、6、7、8、9或10个突变选自表2和/或表6。8.段落2的shc/hac衍生物，所述衍生物包含相对于seqidno.2而言具有最多6个突变并且包含与f182l和/或i498t中的至少任一者或多者组合的至少取代f668y或y185r的氨基酸序列。9.段落7的shc/hac衍生物，所述衍生物包含相对于seqidno.2而言具有最多8个氨基酸改变并且相对于seqidno.2在选自位置129、145、182、185、282、498、647和668的位置中包含一个或多个氨基酸改变的氨基酸序列，其中相对于seqidno.2而言所述shc/hac衍生物具有增加的hac酶促活性。10.段落9的shc/hac衍生物，所述衍生物相对于seqidno.2而言包含一个或多个取代，所述取代选自：s129a、v145v、f182l、y185r、g282v、i498t、h646h和f668y。11.根据段落10的shc/hac衍生物，所述衍生物包含f668y。12.根据段落10的shc/hac衍生物，所述衍生物包含f182l。13.根据段落10的shc/hac衍生物，所述衍生物包含f668y和f182l。14.根据段落10的shc/hac衍生物，所述衍生物包含y185r和i498t。15.根据段落14的shc/hac衍生物，所述衍生物还包含g282v。16.根据段落14的shc/hac衍生物，所述衍生物还包含f668y。17.根据段落14的shc/hac衍生物，所述衍生物还包含f182l。18.根据段落17的shc/hac衍生物，所述衍生物还包含f668y。19.根据段落11的shc/hac衍生物，所述衍生物还包含h646h。20.根据段落10的shc/hac衍生物，所述衍生物包含s129a和v145v和f182l。21.根据前述段落任一项的shc/hac衍生物，所述衍生物具有选自seqidno.41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73和/或75的氨基酸序列。22.一种分离的核苷酸序列，所述核苷酸序列编码根据段落1-21中任一项的shc衍生物。23.根据段落22的分离的核苷酸序列，其中所述核苷酸序列选自seqidno.42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74和/或76。24.一种构建体，所述构建体包含段落22或段落23的核苷酸序列。25.根据段落24的构建体，所述构建体包含与段落22或23的核苷酸序列功能性连接的启动子。26.段落25的构建体，其中所述启动子是诱导型启动子或组成型启动子。27.一种载体，所述载体包含根据段落24-26中任一项的构建体。28.段落27的载体，其中所述载体是质粒。29.根据段落28的载体，所述载体能够在选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞中指导表达。30.段落24-26中任一项的构建体或根据段落27-29中任一项的载体，其中所述构建体或所述载体能够整合进选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞的基因组中。31.一种重组宿主细胞，所述重组宿主细胞包含根据段落22或23的核苷酸序列或根据段落24-26或30中任一项的构建体或根据段落27-30中任一项的载体。32.根据段落31的重组宿主细胞，其中所述宿主细胞选自原核宿主细胞，所述原核宿主细胞由埃希杆菌属(escherichia)、链霉菌属(streptomyces)、芽孢杆菌属(bacillus)、假单胞菌属(pseudomonas)、乳杆菌属(lactobacillus)和乳球菌属(lactococcus)的细菌组成。33.段落32的重组宿主细胞，其中所述宿主细胞是大肠杆菌宿主细胞。34.段落33的重组宿主细胞，其中所述宿主细胞过表达编码所述shc/hac衍生物的基因。35.一种制备根据段落1-21中任一项的shc/hac衍生物的方法，所述方法包括在允许产生所述shc/hac衍生酶的条件下培养一种或多种根据段落31-34中任一项的重组宿主细胞的步骤。36.段落35的方法，其中所述细胞培养在适合生物催化剂产生的条件下发生。37.一种制备(-)-降龙涎醚的方法，所述方法包括使用根据段落31-34中任一项的重组宿主细胞或通过使用包含编码wtshc/hac的seqidno.166的重组宿主细胞将高法呢醇转化为(-)-降龙涎醚，其中如果使用wtshc/hac，则高法呢醇向(-)-降龙涎醚的生物转化用除了tritonx-100的增溶剂进行。38.根据段落37的方法，其中高法呢醇向(-)-降龙涎醚的转化是在适用于wtshc/hac或shc/hac衍生酶的生物转化反应条件下进行。39.根据段落37或38的方法，其中高法呢醇向(-)-降龙涎醚的转化在适用于wtshc/hac或shc/hac衍生酶的ph、温度、增溶剂浓度下发生。40.根据段落39的方法，其中高法呢醇向(-)-降龙涎醚的转化在范围为30℃至60℃的温度下、在范围为约4-8的ph下并且对于wtshc/hac酶在存在除了tritonx-100之外的增溶剂的情况下进行。41.根据段落37-40中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于wtshc/hac或shc/hac衍生酶的反应条件中的一者或多者进行。42.根据段落37-41中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5:1至2:1的范围内，优选约1:1或0.5:1。43.根据段落37-42中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。44.根据段落37-43中任一项的方法，其中所述高法呢醇底物包含一种或多种高法呢醇立体异构体。45.根据段落44的方法，其中所述高法呢醇底物包含两种高法呢醇立体异构体。46.根据段落45的方法，其中所述高法呢醇底物包含ee:ez立体异构体。47.根据段落44-46中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29以及70:30。48.段落47的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。49.段落35或36的方法，其中所述高法呢醇包含重量比为80:20的ee:ez立体异构体混合物。50.段落37-49中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。51.段落37-50中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述生物转化反应混合物分离(-)-降龙涎醚。52.根据段落51的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。53.段落52的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。54.段落52或53的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。55.通过段落51-54中任一项的方法能够获得的(-)-降龙涎醚。56.段落55的(-)-降龙涎醚，其为固体形式，优选为无定形形式或晶体形式。57.一种制备含有(-)-降龙涎醚的产品的方法，所述方法包括将段落55或56的(-)-降龙涎醚掺入所述产品，优选香料产品、美容产品、清洁产品、洗涤剂产品或皂产品。58.一种香料或化妆品或消费者护理产品，其中包含段落55或56的(-)-降龙涎醚。59.一种香料或化妆品或消费者护理组合物，其中包含段落55或56的(-)-降龙涎醚和一种或多种另外的组分。60.段落55或56的(-)-降龙涎醚的用途，其用作香料或化妆品或消费品诸如织物护理、化妆用具、美容护理和/或清洁产品的一部分。61.根据段落1-21中任一项的shc/hac衍生酶、根据段落22或23的核苷酸序列、根据段落24-26或30中任一项的构建体、根据段落27-30中任一项的载体或根据段落31-34中任一项的重组宿主细胞或表达wtshc/hac酶的重组宿主细胞的用途，用于将高法呢醇生物转化为(-)-降龙涎醚，其中所述wtshc/hac酶与除了tritonx-100以外的增溶剂一起用于所述生物转化反应。本发明的另外的方面(zmoshc2)1.一种角鲨烯何帕烯环化酶(shc)/高法呢醇降龙涎香醚环化酶(hac)衍生物，所述衍生物包含相对于seqidno.3具有独立选自取代、缺失或插入的1-50个突变的氨基酸序列。2.根据段落1的shc/hac衍生物，其中所述shc衍生物包含相对于seqidno.3具有1至40个突变、1-30个突变、1-20个突变、1-10个突变或1-6个突变的氨基酸序列。3.根据段落1或段落2的shc/hac衍生物，其中所述shc/hac衍生物包含相对于seqidno.3具有至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的氨基酸序列。4.根据段落3的shc/hac衍生物，其中所述shc变体包含与seqidno.3具有至少95％同一性的氨基酸序列。5.一种shc/hac衍生物，其相对于seqidno.3而言包含独立选自取代、缺失或插入的1-10个突变，其中除了shc活性位点突变之外的所述一个或多个突变位于shc酶的结构域2中(图19和/或20)。6.根据段落1-5中任一项的shc/hac衍生物，其中相对于seqidno.3的所述一个或多个突变选自表3，其中如果仅选择一个突变，则其不是f620y。7.段落6的shc/hac衍生物，其中至少2、3、4、5、6、7、8、9或10个突变选自表3和/或表7。8.段落2的shc/hac衍生物，所述衍生物包含相对于seqidno.3而言具有最多6个突变并且包含与f137l和/或i450t中的至少任一者或多者组合的至少取代f620y或i140r的氨基酸序列。9.段落7的shc/hac衍生物，所述衍生物包含相对于seqidno.3而言具有最多8个氨基酸改变并且相对于seqidno.3在选自位置85、100、137、140、233、450、598和620的位置中包含一个或多个氨基酸改变的氨基酸序列，其中相对于seqidno.3而言所述shc/hac衍生物具有增加的hac酶促活性。10.段落9的shc/hac衍生物，所述衍生物相对于seqidno.3而言包含一个或多个取代，所述取代选自：g85a、v100v、f137l、i140r、v233v、i450t、n598h和f620y。11.根据段落10的shc/hac衍生物，所述衍生物包含f620y。12.根据段落10的shc/hac衍生物，所述衍生物包含f137l。13.根据段落10的shc/hac衍生物，所述衍生物包含f620y和f137l。14.根据段落10的shc/hac衍生物，所述衍生物包含i140r和i450t。15.根据段落14的shc/hac衍生物，所述衍生物还包含v233v。16.根据段落14的shc/hac衍生物，所述衍生物还包含f620y。17.根据段落14的shc/hac衍生物，所述衍生物还包含f137l。18.根据段落17的shc/hac衍生物，所述衍生物还包含f620y。19.根据段落11的shc/hac衍生物，所述衍生物还包含n598h。20.根据段落10的shc/hac衍生物，所述衍生物包含g85a和v100v和f137l。21.根据前述段落任一项的shc/hac衍生物，所述衍生物具有选自seqidno.77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109和/或111的氨基酸序列。22.一种分离的核苷酸序列，所述核苷酸序列编码根据段落1-21中任一项的shc衍生物。23.根据段落22的分离的核苷酸序列，其中所述核苷酸序列选自seqidno.78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110和/或112。24.一种构建体，所述构建体包含段落22或段落23的核苷酸序列。25.根据段落24的构建体，所述构建体包含与段落22或23的核苷酸序列功能性连接的启动子。26.段落25的构建体，其中所述启动子是诱导型启动子或组成型启动子。27.一种载体，所述载体包含根据段落24-26中任一项的构建体。28.段落27的载体，其中所述载体是质粒。29.根据段落28的载体，所述载体能够在选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞中指导表达。30.段落24-26中任一项的构建体或根据段落27-29中任一项的载体，其中所述构建体或所述载体能够整合进选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞的基因组中。31.一种重组宿主细胞，所述重组宿主细胞包含根据段落22或23的核苷酸序列或根据段落24-26或30中任一项的构建体或根据段落27-30中任一项的载体。32.根据段落31的重组宿主细胞，其中所述宿主细胞选自原核宿主细胞，所述原核宿主细胞由埃希杆菌属(escherichia)、链霉菌属(streptomyces)、芽孢杆菌属(bacillus)、假单胞菌属(pseudomonas)、乳杆菌属(lactobacillus)和乳球菌属(lactococcus)的细菌组成。33.段落32的重组宿主细胞，其中所述宿主细胞是大肠杆菌宿主细胞。34.段落33的重组宿主细胞，其中所述宿主细胞过表达编码所述shc/hac衍生物的基因。35.一种制备根据段落1-21中任一项的shc/hac衍生物的方法，所述方法包括以下步骤：(a)在允许产生shc/hac衍生酶的条件下培养一种或多种根据段落31-34中任一项的重组宿主细胞。36.段落35的方法，其中所述细胞培养在适合生物催化剂产生的条件下发生。37.一种制备(-)-降龙涎醚的方法，所述方法包括使用根据段落31-34中任一项的重组宿主细胞或通过使用包含编码wtshc/hac的seqidno.167的重组宿主细胞将高法呢醇转化为(-)-降龙涎醚，其中如果使用wtshc/hac，则高法呢醇向(-)-降龙涎醚的生物转化用除了tritonx-100的增溶剂进行。38.根据段落37的方法，其中高法呢醇向(-)-降龙涎醚的转化是在适用于wtshc/hac或shc/hac衍生酶的生物转化反应条件下进行。39.根据段落37或38的方法，其中高法呢醇向(-)-降龙涎醚的转化在适用于wtshc/hac或shc/hac衍生酶的ph、温度、增溶剂浓度下进行。40.根据段落39的方法，其中高法呢醇向(-)-降龙涎醚的转化在范围为30℃至60℃的温度下、在范围为约4-8的ph下并且对于wtshc/hac酶在存在除了tritonx-100之外的增溶剂的情况下进行。41.根据段落37-40中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于wtshc/hac或shc/hac衍生酶的反应条件中的一者或多者进行。42.根据段落37-41中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5:1至2:1的范围内，优选约1:1或0.5:1。43.根据段落37-42中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。44.根据段落37-43中任一项的方法，其中所述高法呢醇底物包含一种或多种高法呢醇立体异构体。45.根据段落44的方法，其中所述高法呢醇底物包含两种高法呢醇立体异构体。46.根据段落45的方法，其中所述高法呢醇底物包含ee:ez立体异构体。47.根据段落44-46中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29以及70:30。48.段落47的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。49.段落35或36的方法，其中所述高法呢醇包含重量比为80:20的ee:ez立体异构体混合物。50.段落37-49中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。51.段落37-50中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述生物转化反应混合物分离(-)-降龙涎醚。52.根据段落51的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。53.段落52的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。54.段落52或53的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。55.通过段落51-54中任一项的方法能够获得的(-)-降龙涎醚。56.段落55的(-)-降龙涎醚，其为固体形式，优选为无定形形式或晶体形式。57.一种制备含有(-)-降龙涎醚的产品的方法，所述方法包括将段落55或56的(-)-降龙涎醚掺入所述产品，优选香料产品、美容产品、清洁产品、洗涤剂产品或皂产品。58.一种香料或化妆品或消费者护理产品，其中包含段落55或56的(-)-降龙涎醚。59.一种香料或化妆品或消费者护理组合物，其中包含段落55或56的(-)-降龙涎醚和一种或多种另外的组分。60.段落55或56的(-)-降龙涎醚的用途，其用作香料或化妆品或消费品诸如织物护理、化妆用具、美容护理和/或清洁产品的一部分。61.根据段落1-21中任一项的shc/hac衍生酶、根据段落22或23的核苷酸序列、根据段落24-26或30中任一项的构建体、根据段落27-30中任一项的载体或根据段落31-34中任一项的重组宿主细胞或表达wtshc/hac酶的重组宿主细胞的用途，用于将高法呢醇生物转化为(-)-降龙涎醚，其中所述wtshc/hac酶与除了tritonx-100以外的增溶剂一起用于所述生物转化反应。本发明的另外的方面(bjpshc)1.一种角鲨烯何帕烯环化酶(shc)/高法呢醇降龙涎香醚环化酶(hac)衍生物，所述衍生物包含相对于seqidno.4具有独立选自取代、缺失或插入的1-50个突变的氨基酸序列。2.根据段落1的shc/hac衍生物，其中所述shc衍生物包含相对于seqidno.4具有1至40个突变、1-30个突变、1-20个突变、1-10个突变或1-6个突变的氨基酸序列。3.根据段落1或段落2的shc/hac衍生物，其中所述shc/hac衍生物包含相对于seqidno.4具有至少40％同一性、至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性的氨基酸序列。4.根据段落3的shc/hac衍生物，其中所述shc变体包含与seqidno.4具有至少95％同一性的氨基酸序列。5.一种shc/hac衍生物，其相对于seqidno.4而言包含4-10个独立选自取代、缺失或插入的1-10个突变，其中除了shc活性位点突变之外的所述一个或多个突变位于shc酶的结构域2中(图19和/或20)。6.根据段落1-5中任一项的shc/hac衍生物，其中相对于seqidno.4的所述一个或多个突变选自表4，其中如果仅选择一个突变，则其不是f628y。7.段落6的shc/hac衍生物，其中至少2、3、4、5、6、7、8、9或10个突变选自表4和/或表8。8.段落2的shc/hac衍生物，所述衍生物包含相对于seqidno.4而言具有最多6个突变并且包含与f137l和/或i450t中的至少任一者或多者组合的至少取代f628y或i140r的氨基酸序列。9.段落7的shc/hac衍生物，所述衍生物包含相对于seqidno.4而言具有最多8个氨基酸改变并且相对于seqidno.4在选自位置88、104、141、144、241、459、607和628的位置中包含一个或多个氨基酸改变的氨基酸序列，其中相对于seqidno.4而言所述shc/hac衍生物具有增加的hac酶促活性。10.段落9的shc/hac衍生物，所述衍生物相对于seqidno.4而言包含一个或多个取代，所述取代选自：a88a、v104v、f141l、y144r、v241v、i459t、m607h和f628y。11.根据段落10的shc/hac衍生物，所述衍生物包含f628y。12.根据段落10的shc/hac衍生物，所述衍生物包含f141l。13.根据段落10的shc/hac衍生物，所述衍生物包含f628y和f141l。14.根据段落10的shc/hac衍生物，所述衍生物包含y144r和i459t。15.根据段落14的shc/hac衍生物，所述衍生物还包含v241v。16.根据段落14的shc/hac衍生物，所述衍生物还包含f628y。17.根据段落14的shc/hac衍生物，所述衍生物还包含f141l。18.根据段落17的shc/hac衍生物，所述衍生物还包含f628y。19.根据段落11的shc/hac衍生物，所述衍生物还包含m607h。20.根据段落10的shc/hac衍生物，所述衍生物包含s129a和v145v和f182l。21.根据前述段落任一项的shc/hac衍生物，所述衍生物具有选自seqidno.113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145和/或147的氨基酸序列。22.一种分离的核苷酸序列，所述核苷酸序列编码根据段落1-21中任一项的shc衍生物。23.根据段落22的分离的核苷酸序列，其中所述核苷酸序列选自seqidno.114、116、118、120、124、126、128、130、132、134、136、138、140、142、144、146和/或148。24.一种构建体，所述构建体包含段落22或段落23的核苷酸序列。25.根据段落24的构建体，所述构建体包含与段落22或23的核苷酸序列功能性连接的启动子。26.段落25的构建体，其中所述启动子是诱导型启动子或组成型启动子。27.一种载体，所述载体包含根据段落24-26中任一项的构建体。28.段落27的载体，其中所述载体是质粒。29.根据段落28的载体，所述载体能够在选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞中指导表达。30.段落24-26中任一项的构建体或根据段落27-29中任一项的载体，其中所述构建体或所述载体能够整合进选自原核生物、酵母、植物和昆虫宿主细胞的宿主细胞的基因组中。31.一种重组宿主细胞，所述重组宿主细胞包含根据段落22或23的核苷酸序列或根据段落24-26或30中任一项的构建体或根据段落27-30中任一项的载体。32.根据段落31的重组宿主细胞，其中所述宿主细胞选自原核宿主细胞，所述原核宿主细胞由埃希杆菌属(escherichia)、链霉菌属(streptomyces)、芽孢杆菌属(bacillus)、假单胞菌属(pseudomonas)、乳杆菌属(lactobacillus)和乳球菌属(lactococcus)的细菌组成。33.段落32的重组宿主细胞，其中所述宿主细胞是大肠杆菌宿主细胞。34.段落33的重组宿主细胞，其中所述宿主细胞过表达编码所述shc/hac衍生物的基因。35.一种制备根据段落1-21中任一项的shc/hac衍生物的方法，所述方法包括以下步骤：(a)在允许产生shc/hac衍生酶的条件下培养一种或多种根据段落31-34中任一项的重组宿主细胞。36.段落35的方法，其中所述细胞培养在适合生物催化剂产生的条件下发生。37.一种制备(-)-降龙涎醚的方法，所述方法包括使用根据段落31-34中任一项的重组宿主细胞或通过使用包含编码wtshc/hac的seqidno.168的重组宿主细胞将高法呢醇转化为(-)-降龙涎醚，其中如果使用wtshc/hac，则高法呢醇向(-)-降龙涎醚的生物转化用除了tritonx-100的增溶剂进行。38.根据段落37的方法，其中高法呢醇向(-)-降龙涎醚的转化是在适用于wtshc/hac或shc/hac衍生酶的生物转化反应条件下进行。39.根据段落37或38的方法，其中高法呢醇向(-)-降龙涎醚的转化在适用于wtshc/hac或shc/hac衍生酶的ph、温度、增溶剂浓度下发生。40.根据段落39的方法，其中高法呢醇向(-)-降龙涎醚的转化在范围为30℃至60℃的温度下、在范围为约4-8的ph下并且对于wtshc/hac酶在存在除了tritonx-100之外的增溶剂的情况下进行。41.根据段落37-40中任一项的方法，其中高法呢醇向(-)-降龙涎醚的转化使用如表24或表24a中所列的用于wtshc/hac或shc/hac衍生酶的反应条件中的一者或多者进行。42.根据段落37-41中任一项的方法，其中生物催化剂与高法呢醇的重量比在约0.5:1至2:1的范围内，优选约1:1或0.5:1。43.根据段落37-42中任一项的方法，其中细胞培养步骤和生物转化反应步骤在同一反应容器中进行。44.根据段落27-31中任一项的方法，其中所述高法呢醇底物包含一种或多种高法呢醇立体异构体。45.根据段落44的方法，其中所述高法呢醇底物包含两种高法呢醇立体异构体。46.根据段落45的方法，其中所述高法呢醇底物包含ee:ez立体异构体。47.根据段落44-46中任一项的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：100:00；99:01；98:02；97:03；96:04；95:05；94:06；93:07；92:08；91:09；90:10；89:11；88:12；87:13；86:14；85:15；84:16；83:17；82:18；81:19；80:20；79:21；78:22；77:23；76:24；75:25；74:26；73:27；72:28；71:29以及70:30。48.段落47的方法，其中所述高法呢醇包含ee:ez立体异构体混合物，所述ee:ez立体异构体混合物的重量比选自：ee:ez90:10；ee:ez80:20；ee:ez86:14；ee:ez70:30；ee:ez69:31；以及ee:ez66:34。49.段落35或36的方法，其中所述高法呢醇包含重量比为80:20的ee:ez立体异构体混合物。50.段落37-49中任一项的方法，其中(-)-降龙涎醚以与副产物(ii)、(iv)和/或(iii)中的至少一种或多种的混合物产生。51.段落37-50中任一项的方法，其中使用有机溶剂或汽提/蒸馏步骤或过滤从所述生物转化反应混合物分离(-)-降龙涎醚。52.根据段落51的方法，其中使用有机溶剂从所述反应混合物分离(-)-降龙涎醚。53.段落52的方法，其中使用有机溶剂使(-)-降龙涎醚选择性结晶。54.段落52或53的方法，其中所述(-)-降龙涎醚基本上不含副产物(ii)、(iv)和/或(iii)。55.通过段落51-54中任一项的方法能够获得的(-)-降龙涎醚。56.段落55的(-)-降龙涎醚，其为固体形式，优选为无定形形式或晶体形式。57.一种制备含有(-)-降龙涎醚的产品的方法，所述方法包括将段落55或56的(-)-降龙涎醚掺入所述产品，优选香料产品、美容产品、清洁产品、洗涤剂产品或皂产品。58.一种香料或化妆品或消费者护理产品，其中包含段落55或56的(-)-降龙涎醚。59.一种香料或化妆品或消费者护理组合物，其中包含段落55或56的(-)-降龙涎醚和一种或多种另外的组分。60.段落55或56的(-)-降龙涎醚的用途，其用作香料或化妆品或消费品诸如织物护理、化妆用具、美容护理和/或清洁产品的一部分。61.根据段落1-21中任一项的shc/hac衍生酶、根据段落22或23的核苷酸序列、根据段落24-26或30中任一项的构建体、根据段落27-30中任一项的载体或根据段落31-34中任一项的重组宿主细胞或表达wtshc/hac酶的重组宿主细胞的用途，用于将高法呢醇生物转化为(-)-降龙涎醚，其中所述wtshc/hac酶与除了tritonx-100以外的增溶剂一起用于所述生物转化反应。在另一方面，提供了基于具有本文所述的shc或衍生物的氨基酸序列的shc的结构坐标的shc晶体模型结构(cms)。shccms包括角鲨烯/高法呢醇结合口袋结构域(shbd)，该结构域包括角鲨烯/高法呢醇结合口袋(shbp)和结合至sbd的角鲨烯/高法呢醇底物(如参见图19和20)。该shc晶体模型结构(cms)有利于潜在的候选shc/hac衍生酶候选者的计算机测试(in-silicotesting)。因而在又一些其它实施方案中，本公开提供了筛选能够结合至shbd的酶(如shc/hac衍生物)的方法，其中该方法包括使用shc/haccms。在另一个方面，本公开提供了用于筛选能够结合至shbp的酶(如参照shc或shc/hac衍生物)的方法，并且该方法包括使shbp与测试化合物(如shc衍生物)接触并确定所述测试化合物是否结合至所述shbp。在一些实施方案中，该方法是筛选可用于调节shc衍生酶的活性的测试化合物(如调节剂)。在另一个方面，本公开提供了对以下进行预测、模拟或建模的方法：参照shc和/或shc/hac衍生物的分子特征和/或参照shc和/或shc/hac衍生物与角鲨烯/高法呢醇结合结构域(shbd)的分子相互作用，该方法包括使用计算机模型，所述计算机模型包括使用或描述如上文所限定的角鲨烯/高法呢醇结合结构域的结构坐标以提供所述配体结合结构域的图像以及任选地显示所述图像。在本说明书及后面的权利要求书通篇中，除非上下文另行要求，否则词语“包含”及其变型形式如“包括”和“具有”将理解为暗示包括所述整数或步骤或者整数或步骤的组，但不排除任何其它整数或步骤或者整数或步骤的组。术语“包含”还意指“含有”以及“由...组成”，例如组合物“包含”x可排他性地由x组成或者可含有额外的某些东西，如x+y。还必须注意的是，如本文中以及随附的权利要求书中所用的，单数形式“一个”、“一种”、“所述”包括多个指代物，除非上下文另外明确规定。举个例子，提及“基因”或“酶”是提及“一种或多种基因”或“一种或多种酶”。应当理解，本公开不限于本文中所述的特定方法、规程和试剂，因为这些可以变化。还应当理解，本文所用的术语仅用于描述具体的实施方案的目的，并非旨在限制本公开的范围，本公开的范围将仅由随附的权利要求书限制。除非另外限定，否则本文使用的所有科技术语的含义与本领域技术人员通常理解的相同。根据本公开，可能会采用本领域技术内的常规分子生物学、微生物学和重组dna技术。本公开在其应用中不限于下面描述中所述的或附图中所图示的构建的细节以及各组分的布置方式。本公开还可以有其它实施方案并且能够以多种方式实施或能够以多种方式进行。另外，本文所用的措辞和术语是为了描述的目的而不应该视为限制。优选地，本文所用的术语如以下文献中所述进行定义：“amultilingualglossaryofbiotechnologicalterms:(iupacrecommendations)”，leuenberger,h.g.w,nagel,b.和kolbl,h.编辑(1995),helveticachimicaacta,ch-4010basel,switzerland。在本说明书通篇中引用了若干文献。本文中引用的每篇文献(包括所有专利、专利申请、科学出版物、制造商说明书、指南、genbank登录号序列提交等)，无论上文或下文，特此以引用方式全文并入本文。本文所述的实施例示例说明本公开并且无意于对其施加限制。已经根据本公开描述了本公开的不同实施方案。可以对本文所描述和示例说明的技术作出多种修改和改变而不脱离本公开的精神和范围。因此，应当理解实施例仅是示例性的并且不限制本公开的范围。表10：提供了aacshc的登录号表11：提供了zmoshc的登录号表12：示出了来自wo2010139719的其它shc酶的来源表13：wtaacshc氨基酸和核苷酸seqidno.表14：aacshc衍生物氨基酸和核苷酸seqidno.表15：wtzmoshc1和zmoshc1衍生物的氨基酸和核苷酸seqidno.表16：wtzmoshc2和zmoshc2衍生物的氨基酸和核苷酸seqidno.表17：wtbjpshc1和bjpshc1衍生物的氨基酸和核苷酸seqidno.seqidno.1(酸热脂环酸芽孢杆菌)，aacshcmaeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierrseqidno.2(运动发酵单胞菌)，zmoshc1mgidrmnslsrllmkkifgaektsykpasdtiigtdtlkrpnrrpeptakvdktifktmgnslnntlvsacdwligqqkpdghwvgavesnasmeaewclalwflgledhplrprlgnallemqredgswgvyfgagngdinatveayaalrslgysadnpvlkkaaawiaekgglknirvftrywlaligewpwektpnlppeiiwfpdnfvfsiynfaqwaratmvpiailsarrpsrplrpqdrldelfpegrarfdyelpkkegidlwsqffrttdrglhwvqsnllkrnslreaairhvlewiirhqdadggwggiqppwvyglmalhgegyqlyhpvmakalsalddpgwrhdrgesswiqatnspvwdtmlalmalkdakaedrftpemdkaadwllarqvkvkgdwsiklpdvepggwafeyandrypdtddtavalialssyrdkeewqkkgvedaitrgvnwliamqsecggwgafdkdnnrsilskipfcdfgesidppsvdvtahvleafgtlglsrdmpviqkaidyvrseqeaegawfgrwgvnyiygtgavlpalaaigedmtqpyitkacdwlvahqqedggwgescssymeidsigkgpttpsqtawalmgliaanrpedyeaiakgchylidrqeqdgswkeeeftgtgfpgygvgqtiklddpalskrllqgaelsrafmlrydfyrqffpimalsraerlidlnnseqidno.3(运动发酵单胞菌)，zmoshc2mtvstssafhhsplsddvepiiqkatrallekqqqdghwvfeleadatipaeyillkhylgepedleieakigrylrriqgehggwslfyggdldlsatvkayfalkmigdspdaphmlrarneilarggamranvftriqlalfgamswehvpqmpvelmlmpewfpvhinkmaywartvlvpllvlqalkpvarnrrgilvdelfvpdvlptlqesgdpiwrrffsaldkvlhkvepywpknmrakaihscvhfvterlngedglgaiypaiansvmmydalgypenhperaiarraveklmvldgtedqgdkevycqpclspiwdtalvahamlevggdeaeksaisalswlkpqqildvkgdwawrrpdlrpggwafqyrndyypdvddtavvtmamdraaklsdlhddfeeskaramewtigmqsdnggwgafdannsytylnnipfadhgalldpptvdvsarcvsmmaqagisitdpkmkaavdyllkeqeedgswfgrwgvnyiygtwsalcalnvaalphdhlavqkavawlktiqnedggwgencdsyaldysgyepmdstasqtawallglmavgeanseavtkginwlaqnqdeeglwkedyysgggfprvfylryhgyskyfplwalaryrnlkkanqpivhygmseqidno.4(慢生型大豆根瘤菌)，bjpshcmtvtssasaratrdpgnyqtalqstvraaadwlianqkpdghwvgraesnacmeaqwclalwfmgledhplrkrlgqslldsqrpdgawqvyfgapngdinatveayaalrslgfrddepavrrarewieakgglrnirvftrywlaligewpwektpnippeviwfplwfpfsiynfaqwaratlmpiavlsarrpsrplppenrldalfphgrkafdyelpvkagaggwdrffrgadkvlhklqnlgnrlnlglfrpaatsrvlewmirhqdfdgawggiqppwiyglmalyaegyplnhpvlakgldalndpgwrvdvgdatyiqatnspvwdtiltllafddagvlgdypeavdkavdwvlqrqvrvpgdwsmklphvkpggwafeyannyypdtddtavalialaplrhdpkwkakgideaiqlgvdwligmqsqgggwgafdkdnnqkiltkipfcdygealdppsvdvtahiieafgklgisrnhpsmvqaldyirreqepsgpwfgrwgvnyvygtgavlpalaaigedmtqpyigracdwlvahqqadggwgescasymdvsavgrgtttasqtawalmallaanrpqdkdaiergcmwlverqsagtwdepeftgtgfpgygvgqtiklndpalsqrlmqgpelsrafmlrygmyrhyfplmalgralrpqshsseqidno.149(双向伯克霍尔德氏菌)mndltematlsagtvpagldaavasatdallaaqnadghwvyeleadstipaeyvllvhylgetpnleleqkigrylrrvqqadggwplftdgapnisasvkayfalkvigddenaehmqrarraiqamggaemsnvftriqlalygaipwravpmmpveimllpqwfpfhlskvsywartvivpllvlnakrpiaknprgvridelfvdppvnagllprqghqspgwfaffrvvdhalraadglfpnytrerairqavsfvderlngedglgaiypamanavmmydvlgyaedhpnraiarksiekllvvqedeaycqpclspvwdtslaahalletgdaraeeavirglewlrplqildvrgdwisrrphvrpggwafqyanphypdvddtavvavamdrvqklkhndafrdsiararewvvgmqssdggwgafepentqyylnnipfsdhgalldpptadvsgrclsmlaqlgetplnseparraldymlkeqepdgswygrwgmnyvygtwtalcalnaagltpddprvkrgaqwllsiqnkdggwgedgdsyklnyrgfeqapstasqtawallglmaagevnnpavargveyliaeqkehglwdetrftatgfprvfylryhgyrkffplwalaryrnlkrnnatrvtfglseqidno.151(双向伯克霍尔德氏菌)mirrmnksgpspwsaldaaiargrdalmrlqqpdgswcfelesdatitaeyilmmhfmdkiddarqekmarylraiqrldthggwdlyvdgdpdvscsvkayfalkaagdsehaphmvrardailelggaarsnvftrillatfgqvpwratpfmpiefvlfpkwvpismykvaywarttmvpllvlcslkararnprniaipelfvtppdqerqyfppargmrraflaldrvvrhvepllpkrlrqrairhaqawcaermngedglggifppivysyqmmdvlgypddhplrrdcenalekllvtrpdgsmycqpclspvwdtawstmaleqargvavpeagapasaldeldariaraydwlaerqvndlrgdwienapadtqpggwafqyanpyypdiddsavvtamldrrgrthrnadgshpyaarvaraldwmrglqsrnggfaafdadcdrlylnaipfadhgalldpptedvsgrvllcfgvtkraddraslaraidyvkrtqqpdgswwgrwgtnylygtwsvlaglalagedpsqpyiaralawlrarqhadggwgetndsyidpalagtnagestsnctawallaqmafgdgesesvrrgiaylqsvqqddgfwwhrshnapgfprifylkyhgytayfplwalaryrrlaggvsaagahavpastgadaalaseqidno.153(炭疽芽孢杆菌)mllyekaheeivrratalqtmqwqdgtwrfcfegapltdchmifllkllgrdkeiepfvervaslqtnegtwklhedevggnlsatiqsyaallaskkytkedanmkraenfiqerggvarahfmtkfllaihgeyeypslfhlptpimflqndspfsifelsssarihlipmmlclnkrfrvgkkllpnlnhiaggggewfredrspvfqtllsdvkqiisyplslhhkgyeeierfmkeridengtlysyatasfymiyallalghslqssmiqkaiagitsyiwkmergnhlqnspstvwdtallsyalqeaqvskdnkmiqnatayllkkqhtkkadwsvhapaltpggwgfsdvnttipdiddttavlralarsrgnknidnawkkggnwikglqnndggwgafekgvtskllaklpienasdmitdpstpditgrvleffgtyaqnelpekqiqrainwlmnvqeengswygkwgicylygtwavmtglrslgipssnpsltraaswlehiqhedggwgeschssvekrfvtlpfstpsqtawaldalisyydtetpairkgvsyllsnpyvneryptgtglpgafyiryhsyahiyplltlahyikkyrkseqidno.155(桤木弗兰克氏菌)mpagvgvlvwldqrlramgrpdlvtttggaeipfvlvaatastvgvalalrrprhpvgwlflalggvlllsggtqgyaaygavarpgrlpaadlvaiyadagfipwlvlvalilhltptgrplsarwgrialatavagglwllvglvttetmqppfqsvtnplliggplgpllvarrvlglatgagvvlaavslivrfrrsvdverrqllwvavaavplpvlmaasfaasyagnntaaglaaatligllaigaglaigqyhlydveeilsravtyllvsgllaasyatvvivvgqslagrtgrsqisavlatlaavavtapayrkiqegvdrrfsrrrfetlqvirrylrdpdpdvaveevlrralgdptlavaylvddrrqwvsadgqpanpgnsfmaavevyrrgrpiarvtfdrgraqpglvraaataataeldnaglraavalqlvevrqsrtriaaaqfaerrtiernlhdgaqqrllalalqlravqlggdeaslrqaistgidqlqaavvelrelanglhpavladgglaaalddvaartpvpikisapdrryppdleaaawfiaceamanavkhahpttiavdvsapdgqlivevrddgiggaqpsgpglrgiadraeafggsltvhtdpgtgttirallhrrsplssgrrsvmiegcvdvvavrrfrcrssrgsgsrrrrsswrcggicgsrcrtgmsrscsrnaasklitseqidno.157(rhodopseudomonaspalent)mdsilapradaprnidgalresvqqaadwlvanqkpdghwvgraetnatmeaqwclalwflgledhplrvrlgralldtqrpdgawhvfygapngdinatveayaalrslghrddeeplrkardwilskgglanirvftrywlaligewpwektpnilpeviwlptwfpfsiynfaqwaratlmpiavlsahrpsrplapqdrldalfpqgrdsfnydlparlgagvwdvifrkidtilhrlqdwgarrgphgimrrgaidhvlqwiirhqdydgswggiqppwiyglmalhtegyamthpvmakaldalnepgwridigdatfiqatnspvwdtmlsllafddaglgerypeqveravrwvlkrqvlvpgdwsvklpdvkpggwafeyannfypdtddtsvalmalapfrhdpkwqaegiedaiqrgidwlvamqckeggwgafdkdndkkilakipfcdfgealdppsadvtahiieafakvgldrnhpsivraldylkreqepegpwfgrwgvnyvygtgavlpalaaigedmrqpyiaracdwliarqqanggwgescvsymdakqagegtatasqtawalmaliaadrpqdrdaiergclyltetqrdgtwqevhytgtgfpgygvgqtiklndpllskrlmqgpelsrsfmlrydlyrhyfpmmaigrvlrqrgdrsghseqidno.159(天蓝色链霉菌)mtattdgstgaslrplaasasdtditipaaaagvpeaaaratrratdfllakqdaegwwkgdletnvtmdaedlllrqflgiqdeettraaalfirgeqredgtwatfyggpgelsttieayvalrlagdspeaphmaraaewirsrggiasarvftriwlalfgwwkwddlpelppeliyfptwvplniydfgcwarqtivpltivsakrpvrpapfpldelhtdparpnpprplapvaswdgafqridkalhayrkvaprrlrraamnsaarwiierqendgcwggiqppavysvialyllgydlehpvmraglesldrfavwredgarmieacqspvwdtclatialadagvpedhpqlvkasdwmlgeqivrpgdwsvkrpgppggwafefhndnypdiddtaevvlalrrvrhhdpervekaigrgvrwnlgmqskngawgafdvdntsafpnrlpfcdfgevidppsadvtahvvemlaveglahdprtrrgiqwlldaqetdgswfgrwgvnyvygtgsvipaltaaglptshpairravrwlesvqnedggwgedlrsyryvrewsgrgastasqtgwalmallaagerdskavergvawlaatqredgswdepyftgtgfpwdfsinynlyrqvfpltalgryvhgepfakkpraadapaeaapaevkgsseqidno.169atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体101a10(seqidno.30)atggctgagcagttggtggaagcaccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcattacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggctacccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体101a10(seqidno.29)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvhylvetqrpdggwdepyytgtgypgdfylgytmyrhvfptlalgrykqaierr变体111c8((seqidno.28)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcgcgtgggccctgtacccgggtgggccgccggacctcgacacgaccgtcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgctcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体111c8(seqidno.27)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgawalypggppdldttveayvalkyigmsrdeepmqkalrfiqsqggiessrvltrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierr变体shc215g2(seqidno.22)atggctgagcagttggtggaagctccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggaggtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggtgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacaccccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc215g2(seqidno.21)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrrwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldrvlhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhtpfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierr变体shc3(seqidno.26)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggctacccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc3(seqidno.25)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgypgdfylgytmyrhvfptlalgrykqaierr变体shc10(seqidno.32)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgctcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc10(seqidno.31)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvltrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierr变体shc26(seqidno.24)atggctgagcagttggtggaagctccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggaggtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacaccccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc26(seqidno.23)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrrwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhtpfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierr变体shc30(seqidno.34)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgctcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggctacccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc30(seqidno.33)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvltrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgypgdfylgytmyrhvfptlalgrykqaierr变体shc31(seqidno.36)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgctcacgcggaggtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacaccccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc31(seqidno.35)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvltrrwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhtpfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdfylgytmyrhvfptlalgrykqaierr变体shc32(seqidno.38)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggaggtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacaccccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggctacccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc32(seqidno.37)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrrwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhtpfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgypgdfylgytmyrhvfptlalgrykqaierr变体shc33(seqidno.40)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgctcacgcggaggtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacaccccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggctacccaggggatttctacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体shc33(seqidno.39)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvltrrwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhtpfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgypgdfylgytmyrhvfptlalgrykqaierr变体f605w(seqidno.170)atggctgagcagttggtggaagcgccggcctacgcgcggacgctggatcgcgcggtggagtatctcctctcctgccaaaaggacgaaggctactggtgggggccgcttctgagcaacgtcacgatggaagcggagtacgtcctcttgtgccacattctcgatcgcgtcgatcgggatcgcatggagaagatccggcggtacctgttgcacgagcagcgcgaggacggcacgtgggccctgtacccgggtgggccgccggacctcgacacgaccatcgaggcgtacgtcgcgctcaagtatatcggcatgtcgcgcgacgaggagccgatgcagaaggcgctccggttcattcagagccagggcgggatcgagtcgtcgcgcgtgttcacgcggatgtggctggcgctggtgggagaatatccgtgggagaaggtgcccatggtcccgccggagatcatgttcctcggcaagcgcatgccgctcaacatctacgagtttggctcgtgggctcgggcgaccgtcgtggcgctctcgattgtgatgagccgccagccggtgttcccgctgcccgagcgggcgcgcgtgcccgagctgtacgagaccgacgtgcctccgcgccggcgcggtgccaagggagggggtgggtggatcttcgacgcgctcgaccgggcgctgcacgggtatcagaagctgtcggtgcacccgttccgccgcgcggccgagatccgcgccttggactggttgctcgagcgccaggccggagacggcagctggggcgggattcagccgccttggttttacgcgctcatcgcgctcaagattctcgacatgacgcagcatccggcgttcatcaagggctgggaaggtctagagctgtacggcgtggagctggattacggaggatggatgtttcaggcttccatctcgccggtgtgggacacgggcctcgccgtgctcgcgctgcgcgctgcggggcttccggccgatcacgaccgcttggtcaaggcgggcgagtggctgttggaccggcagatcacggttccgggcgactgggcggtgaagcgcccgaacctcaagccgggcgggttcgcgttccagttcgacaacgtgtactacccggacgtggacgacacggccgtcgtggtgtgggcgctcaacaccctgcgcttgccggacgagcgccgcaggcgggacgccatgacgaagggattccgctggattgtcggcatgcagagctcgaacggcggttggggcgcctacgacgtcgacaacacgagcgatctcccgaaccacatcccgttctgcgacttcggcgaagtgaccgatccgccgtcagaggacgtcaccgcccacgtgctcgagtgtttcggcagcttcgggtacgatgacgcctggaaggtcatccggcgcgcggtggaatatctcaagcgggagcagaagccggacggcagctggttcggtcgttggggcgtcaattacctctacggcacgggcgcggtggtgtcggcgctgaaggcggtcgggatcgacacgcgcgagccgtacattcaaaaggcgctcgactgggtcgagcagcatcagaacccggacggcggctggggcgaggactgccgctcgtacgaggatccggcgtacgcgggtaagggcgcgagcaccccgtcgcagacggcctgggcgctgatggcgctcatcgcgggcggcagggcggagtccgaggccgcgcgccgcggcgtgcaatacctcgtggagacgcagcgcccggacggcggctgggatgagccgtactacaccggcacgggcttcccaggggattggtacctcggctacaccatgtaccgccacgtgtttccgacgctcgcgctcggccgctacaagcaagccatcgagcgcaggtga变体f605w(seqidno.171)maeqlveapayartldraveyllscqkdegywwgpllsnvtmeaeyvllchildrvdrdrmekirryllheqredgtwalypggppdldttieayvalkyigmsrdeepmqkalrfiqsqggiessrvftrmwlalvgeypwekvpmvppeimflgkrmplniyefgswaratvvalsivmsrqpvfplperarvpelyetdvpprrrgakggggwifdaldralhgyqklsvhpfrraaeiraldwllerqagdgswggiqppwfyalialkildmtqhpafikgweglelygveldyggwmfqasispvwdtglavlalraaglpadhdrlvkagewlldrqitvpgdwavkrpnlkpggfafqfdnvyypdvddtavvvwalntlrlpderrrrdamtkgfrwivgmqssnggwgaydvdntsdlpnhipfcdfgevtdppsedvtahvlecfgsfgyddawkvirraveylkreqkpdgswfgrwgvnylygtgavvsalkavgidtrepyiqkaldwveqhqnpdggwgedcrsyedpayagkgastpsqtawalmaliaggraeseaarrgvqylvetqrpdggwdepyytgtgfpgdwylgytmyrhvfptlalgrykqaierrseqidno.166(zmoshc1)atgggtattgacagaatgaatagcttaagtcgcttgttaatgaagaagattttcggggctgaaaaaacctcgtataaaccggcttccgataccataatcggaacggataccctgaaaagaccgaaccggcggcctgaaccgacggcaaaagtcgacaaaacgatattcaagactatggggaatagtctgaataatacccttgtttcagcctgtgactggttgatcggacaacaaaagcccgatggtcattgggtcggtgccgtggaatccaatgcttcgatggaagcagaatggtgtctggccttgtggtttttgggtctggaagatcatccgcttcgtccaagattgggcaatgctcttttggaaatgcagcgggaagatggctcttggggagtctatttcggcgctggaaatggcgatatcaatgccacggttgaagcctatgcggccttgcggtctttggggtattctgccgataatcctgttttgaaaaaagcggcagcatggattgctgaaaaaggcggattaaaaaatatccgtgtctttacccgttattggctggcgttgatcggggaatggccttgggaaaagacccctaaccttccccctgaaattatctggttccctgataattttgtcttttcgatttataattttgcccaatgggcgcgggcaaccatggtgccgattgctattctgtccgcgagacgaccaagccgcccgctgcgccctcaagaccgattggatgaactgtttccagaaggccgcgctcgctttgattatgaattgccgaaaaaagaaggcatcgatctttggtcgcaatttttccgaaccactgaccgtggattacattgggttcagtccaatctgttaaagcgcaatagcttgcgtgaagccgctatccgtcatgttttggaatggattatccggcatcaggatgccgatggcggttggggtggaattcagccaccttgggtctatggtttgatggcgttacatggtgaaggctatcagctttatcatccggtgatggccaaggctttgtcggctttggatgatcccggttggcgacatgacagaggcgagtcttcttggatacaggccaccaatagtccggtatgggatacaatgttggccttgatggcgttaaaagacgccaaggccgaggatcgttttacgccggaaatggataaggccgccgattggcttttggctcgacaggtcaaagtcaaaggcgattggtcaatcaaactgcccgatgttgaacccggtggatgggcatttgaatatgccaatgatcgctatcccgataccgatgataccgccgtcgctttgatcgccctttcctcttatcgtgataaggaggagtggcaaaagaaaggcgttgaggacgccattacccgtggggttaattggttgatcgccatgcaaagcgaatgtggcggttggggagcctttgataaggataataacagaagtatcctttccaaaattcctttttgtgatttcggagaatctattgatccgccttcagtcgatgtaacggcgcatgttttagaggcctttggcaccttgggactgtcccgcgatatgccggtcatccaaaaagcgatcgactatgtccgttccgaacaggaagccgaaggcgcgtggtttggtcgttggggcgttaattatatctatggcaccggtgcggttctgcctgctttggcggcgatcggtgaagatatgacccagccttacatcaccaaggcttgcgattggctggtcgcacatcagcaggaagacggcggttggggcgaaagctgctcttcctatatggagattgattccattgggaagggcccaaccacgccgtcccagactgcttgggctttgatggggttgatcgcggccaatcgtcccgaagattatgaagccattgccaagggatgccattatctgattgatcgccaagagcaggatggtagctggaaagaagaagaattcaccggcaccggattccccggttatggcgtgggtcagacgatcaagttggatgatccggctttatcgaaacgattgcttcaaggcgctgaactgtcacgggcgtttatgctgcgttatgatttttatcggcaattcttcccgattatggcgttaagtcgggcagagagactgattgatttgaataattga表18：使用blast和gap程序算法进行的wtaacshc酶相对于wo2010/0139719(巴斯夫公司(basf))中公开的wtshc酶的序列同一性百分比计算.表19：使用blast算法以及huang和miller的算法进行的wtaacshc酶相对于seitz(2012)中公开的wtzmoshc1和wtzmoshc2酶的序列同一性百分比计算.miriamseitz论文可得自http://elib.uni-stuttgart.de/handle/11682/1400表20：高法呢醇的命名表21：反应产物的命名eschers,gierschw.,niclassy,bernardinellog和ohloffg(1990).configuration-odorrelationshipsin5β-降龙涎醚.helv.chim.acta73,1935-1947.附图说明为了更好地理解本公开，参考了附图，其中：图1-4示出了所选择的aacshc衍生物相对于aacshcseqidno.1的序列比对。以降序出现，图1的seqidno.是：seqidno.1、seqidno.29、seqidno.27、seqidno.21、seqidno.19、seqidno.9、seqidno.23、seqidno.33、seqidno.35、seqidno.37和seqidno.39；图5示出了质粒图谱；图6示出了表24中所列出的野生型aacshc和aacshc衍生物在标准条件(ph6.0，55℃，0.050％sds，细胞达到od650nm＝10)下的相对hac活性；图7a示出了采用高法呢醇质量eeh:ezh87:13以及在96％纯度的高法呢醇(用nmr测定)下，aacshc衍生物相对于wtaacshc的hac活性概况；图7b示出了采用高法呢醇质量eeh:ezh87:13以及在96％纯度的高法呢醇(用nmr测定)下，aacshc衍生物相对于wtshc的相对改善(4小时时(初始速度)和22小时时的产率)；图8a示出了采用高法呢醇质量eeh:ezh92:08以及在100％纯度的高法呢醇(用nmr测定)下，aacshc衍生物相对于wtaacshc的hac活性概况；图8b示出了采用高法呢醇质量eeh:ezh92:08以及在100％纯度的高法呢醇(用nmr测定)下，aacshc衍生物相对于wtshc的相对改善(4小时时(初始速度)和22小时时的产率)；图9a示出了采用高法呢醇质量eeh：ezh66：33以及在76％纯度的高法呢醇(通过nmr测定)下，表24中所列出的aacshc衍生物相对于wtaacshc的hac活性概况；图9b示出了采用高法呢醇质量eeh：ezh66：33以及在76％纯度的高法呢醇(通过nmr测定)下，aacshc衍生物相对于wtshc的相对改善(4小时时(初始速度)和22小时时的产率)；图10示出了三种shc衍生物的hac活性结果，这些衍生物较于野生型aacshc/hac酶显示了大约10倍(215g2)、7倍(shc26)和6倍(shc32)的改善。图11示出了由shc/hac衍生物(215g2shc)和wtaacshc催化的e，e-高法呢醇向降龙涎醚转化的观察结果。在反应7小时时(初始反应速度的估计)，使用变体215g2shc时的转化率比使用野生型shc时实现的转化率高13倍。在反应48小时时，用该变体时的转化率是野生型酶的转化率的8倍。图12示出了当eeh用作原料(以供wtshc和/或shc/hac衍生物生物转化)时所产生的反应产物(降龙涎醚和产物(iv))；以及当ee：ez用作原料时所产生的反应产物((-)-降龙涎醚(i)和产物(ii)、(iv)和(iii)(见表21)；为了易于参考，化合物i-iv可鉴定如下：i：(3ar，5as，9as，9br)-3a，6，6，9a-四甲基十二氢萘并[2，1-b]呋喃(-)-ambroxii：(7as，11as，z)-5，8，8，11a-四甲基-2，3，6，7，7a，8，9，10，11，11a-十氢苯并[b]oxonineiv：(3as，5as，9as，9bs)-3a，6，6，9a-四甲基十二氢萘并[2，1-b]呋喃iii：(3ars，5asr，9asr，9bsr)-3a，6，6，9a-四甲基十二氢萘并[2，1-b]呋喃9-表-ambrox图13示出了表25中的降龙涎醚及产物(ii)、(iv)和(iii)的反应产物gc分析；图14示出了表25中的降龙涎醚及产物(ii)、(iv)和(iii)的反应产物gc分析；图15提供了在存在tritonx-100或sds的情况下全细胞生物转化测定法中215g2shc变体活性的对比数据；图16示出了不同sds/细胞比率的eeh转化百分比；图17示出了三种不同sds浓度下标准生物转化反应(如实施例7中所描述的)中的％eeh转化率；图18示出了三种不同ph值下标准生物转化反应(如实施例7中所描述的)中的％eeh转化率；图19示出了shc/hac变体101a10、111c8和215g2中鉴定的突变在shc晶体结构上的位置(以彩色显示)：红色代表变体215g2；紫色(酒红色)代表变体101a10，绿色代表变体111c8。对于鉴定为负责活性增加的氨基酸，侧链在共结晶的底物类似物中以黄色突出显示。所鉴定的变体中不具有改善的活性的其它突变以蓝色标记。应该注意，蓝色突变在酶的两个结构域上约一半对一半(即50:50)散布，而鉴定的有利aacshc突变大部分位于结构域2中。唯一例外是突变f601y，其在活性位点附近；图20示出了以下突变(黑色和白色)：对shc/hac活性不具有有利影响的突变以黑色显示，它们散布在shc酶的两个结构域上。灰色示出了shc变体中鉴定的显示出改善的shc/hac活性的突变(101a10、111c8和215g2)，它们位于shc酶的结构域2中，仅有一个例外。突出显示了引起变体改善活性的突变的侧链；图21示出了质粒pet-28a(+)的克隆和表达区；图21中的序列的seqidno如下：pet28a(核苷酸序列)：seqidno.179；pet28a(氨基酸序列)：seqidno.180；pet28b(核苷酸序列)：seqidno.181；pet28b(氨基酸序列)：seqidno.182；pet28c(核苷酸序列)：seqidno.183；以及pet28c(氨基酸序列)：seqidno.184。图22示出了含有375g/l的细胞、188g/l的eeh、2.33％的sds的1.5倍浓缩eeh生物转化反应物的体积生产力以及与之相比的普通生物转化的体积生产力，该常规生物转化以125g/l的eeh、250g/l的细胞、1.55％的sds平行运行(实施例7)；图23示出了常规的生物转化(125g/l的eeh、250g/l的细胞、1.55％的sds)，其如实施例7中所述运行，但是用0.5％或0.9％nacl替代柠檬酸缓冲液ph5.4，所有其它反应参数不变。在柠檬酸缓冲液中的生物转化平行运行作为对照；图24示出了(-)-降龙涎醚的固相萃取随甲苯洗涤的演变，以初始存在于200ml整个反应液中的(-)-降龙涎醚量的百分比表示(由于反应液/甲苯的体积比，第一次萃取中的百分比超过100％)；以及图25示出了(-)-降龙涎醚的固相萃取随乙醇洗涤的演变，以最初存在的(-)-降龙涎醚量的百分比表示。在4次洗涤后(总共640mletoh，即3.2倍初始整个反应液体积或8倍固相体积)，约99％的初始存在于反应液中的(-)-降龙涎醚得以回收。实施例为了避免疑问，所有提及wtshc和shc变体是提及wtaacshc(seqidno.1)及其变体(如表23和/或表24中所列出的)。实施例1生物催化剂制备方法1shc质粒制备将编码酸热脂环酸芽孢杆菌角鲨烯何帕烯环化酶(aacshc)的基因插入质粒pet-28a(+)中，其中其处于iptg诱导型t7启动子的控制下，用于在大肠杆菌中产生蛋白质(见图5和21)。使用标准的热休克转化方案将该质粒转化进大肠杆菌bl21(de3)菌株中。锥形瓶培养为了产生蛋白质，使用复合培养基(lb)或基本培养基。m9是基本培养基的一个例子，成功地使用了这种培养基。培养基制备对于350ml培养物默认选择的基本培养基制备如下：向35ml柠檬酸/磷酸盐母液(133g/lkh2po4、40g/l(nh4)2hpo4、17g/l柠檬酸.h2o，ph调节至6.3)添加307mlh2o，根据需要用32％naoh将ph调节至6.8。高压灭菌后添加0.850ml50％mgso4、0.035ml痕量元素溶液(组成见下一节中)溶液、0.035ml硫胺素溶液和7ml20％葡萄糖。shc生物催化剂生产(生物催化剂生产)对于小规模生物催化剂生产(野生型shc或shc变体)，用含有shc生产质粒的大肠杆菌bl21(de3)菌株的预培养物接种350ml培养物(补充有50μg/ml卡那霉素的培养基)。在37℃下伴随连续搅拌(250转/分钟)将细胞培养至大约0.5的光密度(od650nm)。然后通过将iptg添加至300μm的浓度，然后伴随连续搅拌另外温育5-6小时来诱导蛋白质产生。最后通过离心收集所得的生物质，用50mmtris-hcl缓冲液ph7.5洗涤。将细胞作为沉淀物在4℃或-20℃下保存直到进一步使用。通常从1升培养物中获得2.5至4克细胞(湿重)，而与所用的培养基无关。制备发酵物并在750mlinforsht反应器中运行。向该发酵容器添加168ml去离子水。该反应容器配备有所有需要的探针(po2探针、ph探针、采样探针、防泡探针)、c+n进料瓶和氢氧化钠瓶并高压灭菌。将以下物质高压灭菌后添加至所述反应器20ml10x磷酸盐/柠檬酸缓冲液14ml50％葡萄糖0.53mlmgso4溶液2ml(nh4)2so4溶液0.020ml痕量元素溶液0.400ml硫胺素溶液0.200ml卡那霉素母液运行参数设定如下：ph＝6.95，po2＝40％，t＝30℃，以300转/分钟搅拌。关联控制参数：转速rpm设定值300，最小300，最大1000，流量l/min设定值0.1，最小0，最大0.6。消泡控制：1:9。用种菌培养物将发酵罐接种至od650nm＝0.4-0.5。将该种菌培养物在lb培养基(+卡那霉素)中于37℃、220rpm下培养8小时。首先以分批模式运行发酵11.5小时，然后开始用进料溶液(无菌葡萄糖溶液(143mlh2o+35g葡萄糖)，添加灭菌后的：17.5ml(nh4)2so4溶液、1.8mlmgso4溶液、0.018ml痕量元素溶液、0.360ml硫胺素溶液、0.180ml卡那霉素母液)进行c+n进料。以大约4.2ml/h的恒定流量运行该进料。在外部进行葡萄糖和nh4+测量以评价培养物中的c源和n源的可用度。通常葡萄糖水平保持十分低。将培养物培养总共大约25小时，这时它们通常达到od650nm＝40-45。然后通过在发酵罐中添加iptg至大约1mm的浓度(使用输注注射器作为iptg脉冲或在3-4小时的周期内添加)，将温度设定至40℃，po2设定至20％来开始shc生产。在40℃下诱导shc产生持续16小时。在诱导结束时，通过离心收集细胞，用0.1m柠檬酸/柠檬酸钠缓冲液ph5.4洗涤并以沉淀物在4℃或-20℃下保存直至进一步使用。结果1a通常，在所有其它条件不变的情况下，与复合培养基相比，在使用基本培养基时所产生的生物催化剂的比活性更高。在30或37℃下成功进行了诱导。注意到当在40-43℃下进行诱导时，获得更高比活力的生物催化剂。结果1b下面的表22为2个实施例示出了诱导开始时和诱导结束时细胞发酵液的培养物体积、光学密度和细胞量以及所收集的生物质的量(湿重)。表22接种时的od650nm：0.45(实施例1)和0.40(实施例2)。起始体积：205ml。实施例2shc变体的制备及活性筛选方法2为了避免疑问，ee对应于(3e,7e)；ez混合物对应于(3z,7e)；ze对应于(7z,3e)；zz对应于(7z,3z)；eeh对应于(3e,7e)。使用野生型(wt)酸热脂环酸芽孢杆菌shc(aacshc)基因作为模板(genbankm73834,swissprotp33247)进行酶进化程序。制备了约10500种shc变体的文库并筛选显示出增加的eeh环化能力的变体。在反应中运行筛选，该反应在含有4g/leeh和0.050％sds的柠檬酸缓冲液(ph6.0)(0.150ml)中、于55℃下并且在连续搅拌下进行。在选取命中进行验证的情况下，在含有4g/leeh、0.050％sds、达到od600nm＝10.0的已表达所述shc变体的细胞的柠檬酸缓冲液ph6.0中运行标准的测试。最终的体积为1ml，将反应物在55℃下温育并且在磁力搅拌器上剧烈搅拌。随时间推移对反应进行采样使得能考察活性概况(eeh转化为(-)-降龙涎醚)并且通过气相色谱分析测定(见下面的分析方法)。根据该验证轮次，获得了具有改善的eeh环化活性的3种变体(101a10、111c8和215g2)并在这3种变体上鉴定了总共8个突变。然后运行突变研究以鉴定这些突变中哪些突变对eeh环化成降龙涎醚是有利的。除了该aacshc衍生物外，还构建了另一种aacshc变体，其含有所有鉴定的有利突变(如下面表23中概述的shc33)。筛选条件为：4g/l的eeh，细胞达到od650nm＝10.0，sds达到0.05％和0.1％(2种浓度)，并且在55℃下在连续搅拌下运行反应。结果2a表23：所评价的aacshc衍生酶中的突变shct77ai92vf129lm132ra224vi432tq579hf601y101a10xx111c8xxx215g2xxxshc3xshc10xshc26xxshc30xxshc31xxxshc32xxxshc33xxxx结果2b在三种选择的突变体(101a10、111c8和215g2)中，215g2显示出最佳的活性。实施例3用于shc变体的最佳反应条件所考察的反应参数：温度、sds浓度和ph方法3就温度、ph和sds浓度分别优化表23中确定的shc变体衍生物的反应条件。为此，用用于产生各变体的质粒转化大肠杆菌细胞，然后如上所述将该大肠杆菌细胞在锥形瓶中培养并诱导shc产生。以这种方式确保了所有的培养物均含有相同或十分相似的shc量。通过离心收集细胞，用0.1m柠檬酸缓冲液(ph6.0)洗涤并在-20℃下保存直至进一步使用。结果3该优化研究的结果汇总于下面的表中。还用野生型shc进行了优化轮次。下面的表24示出了用于野生型以及每种变体的最佳反应条件，这些条件考虑用于表征每种shc/hac衍生酶。表24：shc衍生酶的最佳反应条件shc温度(℃)ph[sds](重量/重量％)wt55(45-60)6.0(5.6-6.2)0.030(0.010-0.075)101a1040(36-50)6.4(5.4-7.0)0.050(0.010-0.10)111c840(36-50)6.0(5.6-6.6)0.070(0.010-0.090)215g235(32-50)5.4(5.0-6.2)0.060(0.010-0.10)shc337(34-50)5.8(5.4-6.4)0.020(0.010-0.060)shc1042(34-55)6.0(5.4-6.4)0.060(0.030-0.10)shc2632(30-50)5.4(5.4-6.2)0.060(0.020-0.10)shc3035(34-50)6.2(5.4-7.0)0.0050(0.0025-0.070)shc3135(30-50)5.6(5.4-6.4)0.050(0.010-0.10)shc3235(34-50)5.6(5.4-6.4)0.050(0.010-0.10)shc3335(32-50)5.2(4.8-6.4)0.030(0.0050-0.10)讨论3实施例3示出了shc衍生物相较于wtshc的反应条件中值得注意的差异。对于shc变体观察到在最佳温度、ph和sds浓度方面与野生型shc有显著的偏差。仅少数突变对最佳生物转化反应条件具有显著影响。为了确定用于所选择的shc变体的各反应条件，在底物载荷为4g/l的eeh并且已产生野生型或shc衍生物的细胞的光密度od650nm＝10.0的条件下运行反应。温度表24中的数据展示了惊人的发现，wtshc酶在55℃(范围为45-60℃)下具有最佳活性时，而许多shc衍生物在35℃(34-50℃)下具有最佳活性。本公开的shc衍生物在较低反应温度下应用于由e,e-高法呢醇制备(-)-降龙涎醚的方法中对于以工业规模生产降龙涎醚具有显著的成本优势。增溶剂从大量不可用于该生物转化反应的可能增溶剂中选择并鉴定了sds(更多信息请参见实施例14)。在反应速度以及产率方面sds优于例如tritonx-100(在以4g/l的eeh进行的测试中以及如实施例7中所提供的在使用125g/l的eeh时均是如此)。实施例4在标准条件下与wtshc酶相比测试shc变体活性方法4为了比较生物催化剂的相对活性，如下描述变体(如表24中所列出的)的产生。用用于产生所述shc变体之一的质粒转化大肠杆菌细胞，然后将该大肠杆菌细胞于37℃和280转/分钟下在lb培养基中培养，让其生长至od650nm＝0.50，然后通过添加iptg诱导酶产生。在37℃、280转/分钟下持续诱导5.5小时。通过离心收集细胞，用0.1m柠檬酸缓冲液(ph6.0)洗涤并在-20℃下保存直至进一步使用。当比较shc变体活性时(见图6)，将反应混合物的样品加载至sds-page凝胶上供分析反应物的shc含量。该分析确认所有反应物均含有相同量的shc酶。结果4a图6示出了野生型和shc变体在标准条件(ph6.0，55℃，0.050％sds，细胞达到od650nm＝10)下的相对活性；还注意到，野生型shc和至少根据本公开实施例的测试shc变体是溶剂耐受的。这意味着可将所选择的水不混溶性溶剂(最高至100％)添加至该生物转化反应。结果4b使用215g2shc变体，当将nacl添加至反应物(测试的浓度为5至100mm(唯一))时，未观察到对该变体活性的明显影响。此外，nacl添加量高达100mm或高达154mm(0.9％nacl)对变体215g2的shc活性未显示出负面影响。这些发现表明，如果在nacl的生理溶液(0.9％)或类似溶液中进行该生物转化反应并且将ph维持在恰当的值(如约5.4(5.2-5.6))，则可在不存在缓冲液但存在生理nacl溶液或类似溶液的情况下进行该生物转化反应。讨论4图6图示了所选择的变体及野生型shc酶在eeh转化为(-)-降龙涎醚方面的活性的等级。实施例5wtshc和shc衍生物活性概况方法5在heidolphsynthesis1设备上在5ml体积的0.1m柠檬酸缓冲液中于900转/分钟的连续摇动下运行活性测试。该反应中所使用的缓冲液的ph、反应运行的温度以及sds(十二烷基硫酸钠)的浓度取决于使用的shc变体(野生型或变体)。所测变体每一者的最佳条件汇总在上面的表24中。使用的高法呢醇原料为96％纯度，高法呢醇底物eeh:ezh比率为87:13。为了避免疑问，ee:ez混合物是(3e,7e)和(3z,7e)异构体的混合物。结果5所使用的高法呢醇：eeh:ezh87:13，纯度(nmr)：96％。在最佳条件下运行的标准测试的结果在图7b(shc衍生物相对于wtshc的活性概况)和图7a中示出，图7a示出了shc衍生物相对于wtshc的相对活性改善(4小时(初始速度)和22小时时的产率)。所使用的高法呢醇：eeh:ezh92:08，纯度(nmr)：100％在最佳条件下运行的标准测试的结果在图8b(aacshc衍生物相对于wtaacshc的活性概况)和图8a中示出，图8a示出了aacshc衍生物相对于wtshc的相对改善(4小时(初始速度)和22小时时的产率)。所使用的高法呢醇：eeh:ezh66:33，纯度(nmr)：76％在最佳条件下运行的标准测试的结果在图9b(如表24中列出的aacshc衍生物相对于wtaacshc的活性概况)和图9a中示出，图9a示出了aacshc衍生物相对于wtshc的相对改善(4小时(初始速度)和22小时时的产率)。讨论5主要结论是与所用的高法呢醇底物的质量无关，四种最好的shc衍生酶以如下次序排序：215gshc、shc26、shc32和shc3。实施例6从用溶剂完全萃取的反应物测定质量平衡方法6所有条件不变，对每种变体运行2次反应。将高法呢醇用作底物。在温育4小时和22小时后，对于每种变体，用等体积的叔丁基甲基醚(mtbe/tbme)总共洗涤6次完全萃取反应产物和未反应的底物。通过gc分析测定每次洗液的高法呢醇和降龙涎醚含量。从校准曲线计算所形成的降龙涎醚和剩余的高法呢醇的总量，校准曲线使用由认证的降龙涎醚和高法呢醇制成的溶液制得。结果6图10中的结果显示，在使用所述底物的情况下，确认了3种最好的变体，它们较于野生型shc酶显示出大约10倍(215g2)、7倍(shc26)和6倍(shc32)的提高。实施例7125g/le,e-高法呢醇(eeh)下的生物转化性能方法7使用215g2shc变体，解决了增加体积生产力的目标。运行系列实验设计(doe)研究以优化包括参数ph、细胞浓度和sds浓度在内的测试反应条件。反应条件为：125g/l的eeh(来自ee:ez86:14的高法呢醇)、250g/l的细胞、1.55％的sds，反应在0.1m柠檬酸缓冲液(ph5.4)于35℃下运行。典型的反应(150g总量)设定如下：在0.75升infors发酵罐中。将该反应容器加载适量的对应18.75geeh的高法呢醇。从在0.1m柠檬酸缓冲液(ph5.4)中制备的15.5％(w/w)溶液添加2.33gsds。通过将已产生215g2shc变体的大肠杆菌细胞悬浮于0.1m柠檬酸缓冲液(ph5.4)中而由该细胞制备细胞悬液。在通过于10℃和17210g下离心10分钟来测定该悬液的细胞湿重后，将适当体积的细胞添加至反应容器以将37.5g的细胞引入该反应中。用所需量的反应缓冲液将反应物的量补足至150g。在900转/分钟的连续搅拌下于37℃运行该反应。使用水中的40％柠檬酸完成ph调节。随时间推移对反应进行采样(1ml)，用5体积的mtbe/tbme(5ml)萃取。在通过离心(台式离心机，13000转/分钟，2分钟)使溶剂相澄清、10倍稀释于mtbe/tbme中后，通过gc分析测定反应物的高法呢醇和降龙涎醚含量。用已产生野生型shc酶的大肠杆菌细胞进行相同的反应。在该情况中，在0.1m柠檬酸缓冲液(ph6.0)中于55℃下运行该反应。下面的表24a的第2行提供了用于该实施例的反应条件概要。表24a第1行中给出的反应条件取自前面的实施例(如实施例3-5)。表24a：第2行示出了用于实施例7的反应条件结果7图11示出了所观察到的由这2种酶催化的eeh向降龙涎醚的转化。在反应7小时时(初始反应速度的估计)，使用变体215g2shc时的转化率比使用野生型shc时实现的转化率高13倍。在反应48小时时，用该变体时的转化率是野生型酶的转化率的约8倍。总体评论7细胞浓度该实施例中所述的反应中的所有细胞浓度(g/l)均以细胞湿重表示。在将该细胞悬液样品在17210g和4℃下离心10分钟后测定细胞悬液的浓度，以细胞湿重(g/l)表示。细胞(g/l)与od650nm之间的相关性使用125g/l的eeh，用215g2shc或wtshc进行生物转化的情况下，该反应中的250g/l细胞对应于该反应中约172的od650nm。当测试不同生物催化剂制备物时，观察到od650nm与生物催化剂量的比率的变化。当生物催化剂用于4g/leeh下的标准测试中但施加细胞至od650nm＝10.0时，据估计od650nm＝10.0相当于1.45g/l的细胞。讨论7数据证明，与本领域的公开内容相比，已使用相对较高的eeh底物浓度(125g/l)发展了优化且有效的hac生物转化工艺，其中在所述本领域中仅公开了约0.2g/l(参见jp2009060799)至约2.36g/l(10mm)的高法呢醇底物浓度(参见wo2010/139719a2、us2012/0135477a1，以及seitz等人(2012)(如上文所引用的))。实施例8gc分析方法8用适当体积的叔丁基甲基醚(mbte/tbme)萃取样品供定量它们的eeh和降龙涎醚含量。通过离心从水相分离溶剂级分，然后用气相色谱分析。将1μl的溶剂相注入(分流比为3)于30mx0.32mmx0.25μmzebronzb-5柱上。使用温度梯度：100℃，15℃/min至200℃，120℃/min至240℃，240℃4分钟，以恒定流量(4ml/minh2)展开柱，这导致降龙涎醚、eeh和ezh分离。进样口温度为200℃，检测器温度：300℃。用以下公式由对应于降龙涎醚和eeh的峰的面积计算eeh转化率：转化率(％)＝100x(面积降龙涎醚_峰/(面积降龙涎醚_峰+面积e,e-高法呢醇峰))通过gc-ms确认反应产物降龙涎醚(记录的值和强度：m/z221(100％),m/z97(40％),m/z137(3.3％),m/z43(2.6％),m/z41(2.5％),m/z55(2.4％),m/z95(1.9％),m/z67(1.8％),m/z81(138％),m/z222(1.7％))。讨论8通过溶剂萃取或汽提进行产品回收。所用的溶剂为例如mtbe或己烷:异丙醇(3:2)。用等体积的溶剂反复萃取反应物并对溶剂级分进行gc分析直至不再检测到底物或产物。一般而言，5到6次洗涤就足够了。备选地，通过汽提进行反应产物的萃取。实施例9一锅法反应方法9使用上述标准的培养和诱导方案，用转化了pet28a(+)215g2shc质粒的大肠杆菌bl21(de3)运行200ml的发酵，该质粒用于产生带有n末端his标签的215g2shc。在诱导阶段结束时，关闭通气，将温度设定为35℃，用柠檬酸将ph调节至5.5，将搅拌器速度设定为500转/分钟。根据培养物生长期间所做的所有添加(进料和基本消耗)估计培养物的体积。根据该体积以及根据培养物的od，向发酵罐添加适量的sds。eeh添加至4g/l。随时间推移对反应进行采样，用700μlmtbe萃取样品(150-300μl)供gc分析。在培养发酵液中eeh直接被转化为降龙涎醚。该反应总共运行22.5天，在这期间反复添加eeh。结果9当达到完成时，10.6g的eeh已环化成降龙涎醚。通过汽提萃取反应产物(下面提供结构)并从反应混合物定量回收。反应产物的注释当由shc转化高法呢醇ee:ez87:13时，产生了如图12中所列出的反应产物降龙涎醚、(ii)、(iv)和(iii)，并且反映该原料的ee:ez比率。当eeh用作原料时，仅生成(-)-降龙涎醚(i)和产物(iv)。当ezh(3z,7e)用作原料时，仅生成产物(ii)和(iii)。然而，当使用eeh和ezh的混合物时，生成降龙涎醚(i)以及产物(ii)、(iv)和(iii)。如果发生ee:ez66:34的100％转化，则这将提供66％:34％的((降龙涎醚+(iv)):((ii)+(iii))。当进行汽提时，其萃取全部4种产物-降龙涎醚以及产物(ii)、(iv)和(iii)，并且结晶步骤产生纯度为99％(gc)的降龙涎醚，产率至少为70％。讨论9数据证明，在生物转化反应或“一锅法”反应系统中生产(-)-降龙涎醚是可能的，并且在汽提和结晶后实现降龙涎醚的选择性富集。如果高法呢醇原料是ee和ez(如86:14)异构体的混合物，则从这些异构体每一者产生2种产物(总共4种)，其中(-)-降龙涎醚目前是粗产物中的主要成分，并且是结晶材料中的优势成分(纯度为99.1％)。未检测到(+)-降龙涎醚。实施例10ee:ez高法呢醇混合物的转化为了避免疑问，ee对应于(3e,7e)；ez混合物对应于(3z,7e)；ze对应于(7z,3e)；zz对应于(7z,3z)；eeh对应于(3e,7e)；ezh对应于(3z,7e)。方法10在以下反应条件下将ee:ez混合物生物转化：146g/l总高法呢醇，使用250g/l的细胞和1.55％的sds，采用以下高法呢醇底物(ee:ez高法呢醇混合物)：ee:ez86:14(该实施例的最高eeh含量)ee:ez69:31(该实施例的最低eeh含量)ee:ez80:20eeh:ezh70:307e,3e/7e,3z高法呢醇混合物的生物转化使用以下反应条件进行生物转化：使用高法呢醇底物(其为7e,3e:7e,3z86:14的混合物)、250g/l的细胞(根据实施例1的方法制备)和1.55％的sds，在容纳有146g/l总高法呢醇的inforsht750ml发酵罐中于0.1m柠檬酸/柠檬酸钠缓冲液(ph5.4)中进行反应(150.1g总量)。伴随连续搅拌(800转/分钟)在35℃下运行该反应，用水中的10至40％柠檬酸进行ph控制。随时间推移对反应混合物进行采样，对样品进行溶剂萃取供gc分析。注意到，使用2种质量的高法呢醇(ee:ez86:14和ee:ez69:31)时高法呢醇转化同样地快。结果10当使用wtshc和一种特异性的shc衍生物(215g2shc)进行125g/l来自eeh:ezh86:14材料的e,e-高法呢醇的生物转化时，观察到e,e--高法呢醇和e,z-高法呢醇二者的转化。也就是说，来自酸热脂环酸芽孢杆菌的野生型shc酶由eeh:ezh86:14材料产生的反应产物与来自表23的shc变体由eeh:ezh混合物产生的反应产物一样(即降龙涎醚、产物(ii)、(iv)和(iii))。图13和图14提供了降龙涎醚及产物(ii)、(iv)和(iii)的反应产物gc分析。讨论10根据本公开的高法呢醇向降龙涎醚的生物转化产生(-)-降龙涎醚作为优势化合物，但也可能会产生如上文所鉴定的非(-)-降龙涎醚的化合物(如化合物(ii)、(iv)和(iii))，这些化合物可能会或可能不会给(-)-降龙涎醚产品赋予令人愉悦的嗅觉香型。如上文所展示的，在选择性结晶条件下，降龙涎醚可与其它副产物((ii)、(iv)和(iii))分离。因此，如果产物对降龙涎醚终产物的感官特性产生负面效果，则从(-)-降龙涎醚终产物选择性分离产物(ii)、(iv)和(iii)可增加其作为香料或风味剂或化妆品或消费者护理产品的价值。使用由经训练的香水师进行的已确定的感官测试进行感官分析。如果(-)-降龙涎醚终产物自身主要负责所需的感官特性的话，则其纯度可以是该产物嗅觉品质的指标。实施例11从ee:ez:ze:zz-高法呢醇混合物进行的eeh转化方法11将ee:ez:ze:zz-高法呢醇40:26:20:14用作底物，用于215g2shc进行的eeh转化。为了进行比较，还使用了ee:ez2:1或93:07的其它高法呢醇。使用215g2shc变体但不在最佳条件下，考察了ee:ez:ze:zz-高法呢醇混合物的转化。反应条件为ph5.8、在100mm柠檬酸盐缓冲液中、0.10％的sds、40℃。观察到以下的eeh转化率，所有的反应均用恒定的2g/l的eeh运行(因此总高法呢醇浓度有变化)。结果11观察到以下高法呢醇异构体混合物转化率：ee:ez2:150-55％ee:ez93:778％ee:ez:ze:zz40:26:20:146％讨论11除了观察到的产率，数据还证明，215g2shc变体能够从复杂的ee:ez:ze:zz高法呢醇混合物将eeh转化为降龙涎醚。正如预期的，观察到较低的转化率，从而导致较低的降龙涎醚产率。该结果与以下观点一致：除了eeh之外的高法呢醇异构体可与eeh竞争对shc/hac衍生酶的接近，从而可能充当eeh向(-)-降龙涎醚转化的竞争性抑制剂和/或备选的底物。实施例12使用tritonx-100和sds时全细胞生物转化的对比数据方法12根据实施例4的方法4中的方案培养大肠杆菌宿主细胞。根据实施例4中的标准测试进行使用215g2shc变体的生物转化反应。选择以下条件作为215g2shc变体的最适反应条件：4g/l的高法呢醇底物，细胞达到od650nm＝10.0，在0.1m柠檬酸/磷酸钠缓冲液ph5.4中，35℃，以及0.07％的sds。结果12图15提供了在使用浓度范围为0.005％至0.48％的tritonx-100和浓度为0.07％的sds时全细胞生物转化测定法中215g2shc变体活性的比较。讨论12数据证明，使用tritonx-100时最大的活性仅为使用sds时获得的活性的约20％。实施例13sds/细胞比率方法13使用4g/l的eeh底物、od650nm＝5.0的已产生215g2shc衍生酶的细胞，根据实施例4中的方法4建立生物转化反应。结果13结果在图16中给出，该图示出了对于不同sds/细胞比率的eeh转化百分比。图16证明，使用不同的sds/细胞比值，eeh向(-)-降龙涎醚转化的百分比取决于sds/细胞比率。必须小心设定该比率以实现最大转化。例如，如果sds浓度过低，则可能会观察到次优的高法呢醇转化。另一方面，例如，如果sds浓度过高，则存在通过破坏完整的微生物细胞和/或shc/hac酶变性/失活而影响生物催化剂的风险。当使用125g/l的eeh和250g/l生物催化剂根据实施例7中的方法7进行生物转化反应时，最好的生物转化方案显示出16:1的[sds]/[细胞]比率。讨论13该结果证明，在增溶剂(如sds)浓度、生物质的量以及底物(eeh)浓度之间存在一定程度的相互依赖性。举个例子，当高法呢醇底物浓度增加时，发生有效生物转化反应需要足够量的生物催化剂和增溶剂(sds)。实施例14供用于生物转化反应的可能增溶剂的测试方法14在使用与标准测试中相同的条件(4g/l的eeh，细胞达到od650nm＝10.0)的215g2shceeh环化反应中测试各种增溶剂(如下面表26中所列出的)作为sds的可能替代物。还使用标准测试对通过将处于其最佳浓度(0.060-0.070％)的sds与所用的其它增溶剂(使用的浓度是根据用这些化合物进行的筛选分别测定为最佳的浓度(见下面的表26))组合来增强活性(累加效果)的可能性进行了测试。此外，还测试了已知可帮助增溶水不溶性化合物的某些“深共熔溶剂”(deepeutecticsolvent)和离子液体。结果14下面的表26汇总了目前在215g2shceeh环化反应中测试的增溶剂(如：表面活性剂、去垢剂、溶解增强剂等)。与使用浓度范围为0.060-0.070％的sds进行的对照反应相比，均没有改善的活性。在单独以定义为最佳的浓度使用这些化合物时观察到的活性仅为用sds进行的对照反应中所获得的活性的约20％。注意到当完全不添加增溶剂时，实现了20％的eeh转化。当使用sds并添加另外的增溶剂(以在测试中定义为最佳的浓度使用)时，没有观察到协同效应。相反，观察到eeh转化百分率降低。从该研究可以得出结论，在测试条件下所述化合物根本不改善eeh转化；相反获得对环化的不利影响，并且sds是所研究的增溶剂中最有用的。此外，从使用已知可帮助增溶水不溶性化合物的“深共熔溶剂”和离子液体的测试未获得积极的结果。表26：提供了在生物转化反应中测试的增溶剂的列表讨论14本申请人从大量不可用于本公开的高法呢醇向(-)-降龙涎醚的生物转化反应中的其它增溶剂中选择了sds并将其鉴定为可用的增溶剂。实施例15对生物转化反应中的sds浓度的敏感性方法15所应用的条件是在125g/l的eeh与250g/l生物催化剂以及1.55％的sds下的标准生物转化(如实施例7中所述)的条件。还测试了两种其它sds浓度(1.40％和1.70％的sds)。所有的sds浓度均为重量/重量％。还将125g/l的eeh与250g/l生物催化剂以及1.55％的sds下的标准生物转化反应条件(如实施例7中所述)用于测试不同的ph值。在0.1m柠檬酸缓冲液中于ph5.4下运行对照。用0.1m乙酸缓冲液运行在更低ph下运行的反应。结果15图17中的数据证明，该生物转化反应看起来对sds浓度改变的敏感性低于当在标准测试(4g/l的eeh并且细胞达到od650nm＝10.0)中测试hac活性时。图18中的数据证明，当应用该生物转化反应时，系统看起来对ph变化的敏感性低于当在标准测试(4g/l的eeh并且细胞达到od650nm＝10.0)中测试hac活性时。讨论15数据证明了在125g/l的eeh和250g/l的细胞下的生物转化反应相对于所测试的sds浓度范围和ph范围的稳健性。实施例16所鉴定的shc/hac突变在晶体结构上的位置在aacshc/hac变体中鉴定的突变的位置在图19中标记如下：红色代表变体215g2；紫色(酒红色)代表变体101a10，绿色代表变体111c8。对于鉴定为负责活性增加的氨基酸，侧链在共结晶的底物类似物中以黄色突出显示。所鉴定的变体中不具有改善活性的其它突变以蓝色标记。应该注意，蓝色突变在酶的两个结构域上约一半对一半(即50:50)散布，而鉴定的有利aacshc突变大部分位于结构域2中。唯一例外是突变f601y，其在活性位点附近。如果仅考虑shc/hac衍生酶215g2和111c8二者，则所有的突变体均位于结构域2中。图20提供了以黑色和白色显示的相同信息。结果16对应于215g2、111c8和101a10的所有有利的突变体(红色/绿色/紫色)大部分(除了一个突变体f601y外)位于shc晶体结构(如图19中所提供的)的结构域2(wendt等人(1997)science277:1811)。根据野生型aacshc(seqidno.1)对shc有利突变组合进行编号。讨论16该晶体结构可用于鉴定具有所需结构/活性关系(尤其是与高法呢醇向(-)-降龙涎醚转化相关的结构/活性关系)的shc/hac衍生物。有用的预选步骤可能要将选择限制在位于shc/hac晶体结构的结构域2中的氨基酸残基(见图19和20)。实例17高法呢醇的制备方法17一般分析条件非极性gc/ms：50℃/2min，20℃/min200℃，35℃/min270℃。带有hp7890a系列gc系统的gc/msagilent5975cmsd。非极性柱：购自sge的bpx5，5％苯基95％二甲基聚硅氧烷，0.22mmx0.25mmx12m。载气：氦。进样口温度：230℃。分流比：1:50。流量：1.0ml/min。传输线：250℃。ms四极杆：106℃。ms源：230℃。a)thf中的mnu的制备将脲(175g,2.9mol)和盐酸甲胺(198g,2.9mol)在水(400ml)中的溶液在搅拌下回流加热(105℃)3.5小时。在40℃下添加溶解于水(200ml)中的nano2(101g,1.45mol)。15分钟后添加thf(1000ml)，得到透明的两相混合物。在1.5小时内在0-5℃及搅拌下添加浓h2so4(110g,1.1mol)。于0-5℃下另外0.5小时后，于25℃分离两个透明相。将有机相(a)(1065ml，理论上为1.35m)在0-5℃下保存数天或立刻加入环丙烷化反应器中。相分离后用thf(2x1:l)萃取水相两次。这得到1100ml的相b和1075的相c。其中相a在后续的环丙烷化反应中有51％的末端链烯转化为环丙烷，相b产生<0.5％的环丙烷，相c不产生可检测的转化。我们得出结论，在第一次相分离后萃取了>99％mnu。因而水相通常在第一次相分离(与有机相a相分离)后在用浓koh水溶液和乙酸处理后弃去。b)使用thf中的mnu制备e-△-法呢烯在0℃下将thf中的1.35mn-甲基-n-亚硝基脲(136ml,184mmol)滴加至0-5℃的快速搅拌的e-β-法呢烯(cas18794-84-8)(25g,122mmol)和koh水溶液(50ml,40％)混合物。在添加4ml的mnu溶液后，添加预先溶解于0.5ml二氯甲烷中的pd(acac)2(7.4mg,0.024mmol,0.02％)。在0-5℃下用4小时添加剩余的mnu溶液。该阶段的gc显示了28％的未转化的e-β-法呢烯、65％的所需单环丙烷(如上所示)以及3％的双环丙烷化化合物5。25℃下16小时后在0-5℃下添加乙酸(100ml)，然后添加叔丁基甲基醚(250ml)。在相分离后用2mhcl(250ml)洗涤有机相并用叔丁基甲基醚(250ml)萃取水相。用水(2x100ml)、10％naoh水溶液(2x100ml)和水(2x100ml)洗涤合并的有机层，用mgso4干燥，过滤并浓缩而得到26.9g微黄的液体，该液体含有9％的e-β-法呢烯、82％的所需单环丙烷化合物和6％的双环丙烷化副产物。可通过蒸馏纯化进一步分离所需化合物。添加1gk2co3(1g)并在135-145℃下于40-60毫巴下在30cm钢卷曲柱上蒸馏得到147g单环丙烷化合物(68％corr)。汇集级分而得到92g100％纯度的单环丙烷化合物。e-△法呢烯的分析数据：1h-nmr(cdcl3,400mhz)：5.1(2m,2h),4.6(2h),2.2(2h),2.1(4h),2.0(2h),1.7(s,3h),1.6(2s,6h),1.3(1h),0.6(2h),0.45(2h)ppm.13c-nmr(cdcl3,400mhz):150.9(s),135.1(s),131.2(s),124.4(d),124.1(d),106.0(t),39.7(t),35.9(t),26.7(t),25.7(q),17.7(q),16.0(d),6.0(t)ppm.gc/ms：218(2％、m+),203(5％、[m-15]+),175(11％),147(31％),134(15％),133(20％),121(12％),107(55％),95(16％),93(30％),91(20％),82(11％),81(33％),79(42％),69(100％),67(22％),55(20％),53(21％),41(75％).ir(薄膜)：3081(w),2967(m),2915(m),2854(m),1642(m),1439(m),1377(m),1107(w),1047(w),1018(m),875(s),819(m),629(w).c16h26的分析计算值：c,88.00；h,12.00。实测值：c,87.80；h,12.01。c)(7e)-4,8,12-三甲基十三烷-3,7,11-三烯-1-醇((7e)-高-法呢醇)的制备在150℃下搅拌加热(e)-(6,10-二甲基十一烷-1,5,9-三烯-2-基)环丙烷(e-△法呢烯)(1g,4.6mmol)、十二烷(0.2g，1.15mmol，内标)和l-(+)-酒石酸(1g,6.9mmol)在压力管中的混合物。在18小时且完全转化(根据gc)后，将该混合物倾注于水(50ml)和甲苯(50ml)上。分离各相并用甲苯(50ml)萃取水相。用浓na2co3水溶液(50ml)和浓nacl溶液(2x50ml)洗涤合并的有机层，用mgso4干燥，过滤并减压蒸发而得到棕色树脂(1.35g)，将其与30％koh水溶液(4.3ml)混合并在25℃下搅拌2小时。根据内标，gc分析表明形成了96％的(7e)-4,8,12-三甲基十三烷-3,7,11-三烯-1-醇。e/z比率为68:22。e异构体的分析数据与来自文献的分析数据一致，参见例如p.kocienski,s.wadmanj.org.chem.54,1215(1989)。结果17数据证明了高法呢醇的制备，该高法呢醇适于生物转化为(-)-降龙涎醚。讨论17这种制备高法呢醇的方法也在两件共同未决的专利申请pct/ep2014/072882(wo2015/059290)和pct/ep2014/072891(wo2015/059293)中详细的描述，将这两篇专利申请的全部内容以引用的方式并入本文。实例18一锅法反应在该实验中：(i)用产生215g2shc变体的大肠杆菌菌株发酵(如实施例1中所述)，然后(ii)直接在发酵液中进行eeh转化。因为3个参数[细胞]、[eeh]和[sds](g/l)相关联，因此需要根据在发酵结束时获得的细胞浓度(g/l)来调节可用体积发酵液中参数[eeh]和[sds]。目标是在1.55％的sds浓度下用250g/l的细胞转化125g/l的eeh。为了进行适当的生物转化，细胞必须在葡萄糖耗竭状况中处于休眠状态。关闭通气。方法18发酵：为了允许相当精确地测定发酵结束时反应器中的发酵液体积，对取出样品的体积以及添加进反应器的所有添加物(进料、碱、酸...)的体积进行记录。发酵液中细胞浓度的测定：在连续搅拌下取出发酵液样品(5-10ml)供细胞湿重(g/l)测定并放进离心管中。记录样品质量。将样品在17210g和4℃下离心10分钟(如12000转/分钟，ss-34转子，sorvallrc3b离心机)。通过小心的吸移取出上清液并记录离心沉淀物的质量。细胞湿重浓度测定为g细胞/l发酵液或g细胞/g发酵液。根据所有的添加物和取出物确定发酵罐中的发酵液体积。在发酵罐处于天平上的情况中，通过称重确定发酵液的质量，如果不是这种情况则假定1ml＝1g。高法呢醇和sds的所需量的确定：根据所测定的细胞浓度和发酵液的体积，确定要添加至反应器的e,e-高法呢醇和sds的量以便保持相同的如实施例9中所述生物转化中列出的3者之间的比率：125g/l的eeh、250g/l的细胞、1.55％的sds。建立生物转化：1.将温度设为35℃。关闭通气。2.向发酵液添加计算量的高法呢醇。3.由含水的15.5％sds母液小心添加所需量的sds。4.将反应物以800转/分钟充分混合大约15分钟。5.记录该反应物的ph(内部ph电极)。6.吸取样品(大约1ml)至15ml的falcon管。添加大约5ml去离子水，在彻底混合后记录经外部校准的电极处的ph。7.使用85％h3po4将反应器中的ph逐步设定至5.4(在经外部校准的电极处测量的值)，同时如上所述定期控制外部电极处的ph(6.)。8.在生物转化期间用例如10-25％h3po4和32％naoh调节ph。9.反应物采样：将大约1ml反应混合物放入15mlfalcon管中。添加大约5mlmtbe。在剧烈振荡下萃取样品。10.将等分试样在台式离心机中以最大速度离心1分钟(埃彭道夫管)。将100μl溶剂相添加至装有900μlmtbe的gc管中。在生物转化的第一天中每1-1.5小时取样。在接下来的日子里仅每天取3份样品。11.如实施例8中所述分析1μl溶剂相的降龙涎醚和eeh含量。12.eeh转化率(％)计算为100x(降龙涎醚面积/(降龙涎醚面积+eeh面积))。结果18结果证明，以1.9升的规模在klf2000反应器(生物工程公司(bioengineering))中进行了一锅法发酵+eeh转化。251g/l的细胞使得在47小时内238geeh(251g/l的细胞)转化达到≥93％。当在开始后93小时测量时，转化率为99％。在inforsht0.75l反应器中进行了类似的一锅法实验。在根据标准方案(实施例1)的发酵后，添加从用相同方案平行运行的其它发酵收集而来的反应器细胞。所得的发酵液量为479g。细胞浓度测定为313.7g/l，其为常规生物转化中的细胞浓度(250g/l的细胞)的1.25倍。相应地将eeh和sds添加至该反应器。在少于90小时内，75.1geeh(在该实施例中等价于157g/l的eeh)转化达到98％。该结果证明，有可能在≥125g/l的eeh下运行一锅法发酵+eeh转化，只要发酵轮次以足够高的细胞密度提供已产生215g2shc变体的细胞。讨论18有利的是，获得了99％的底物转化率，当使用昂贵的原料(如eeh)时这在商业上是非常有用的。实施例19增加体积生产力.方法19为了进一步增加体积生产力，运行了1.5倍浓缩的生物转化，其含有375g/l的细胞、188g/l的eeh、2.33％的sds。以125g/l的eeh、250g/l的细胞、1.55％的sds平行运行常规的生物转化(实施例7)。在inforsht0.750l反应器中运行这两个反应，所有其它参数不变。结果19图22中的结果证明，在开始后75小时所述1.5倍生物转化中的转化率百分比为88％，相比之下在常规生物转化中为95％。在开始后96小时所述1.5倍生物转化中的转化率百分比为93％的eeh转化，相比之下在常规生物转化中为97％。所述1.5倍生物转化中的转化率百分比为常规生物转化中获得的转化率百分比的96％。注意到，在1.5倍生物转化中搅拌随时间推移变得更困难，因为油状高法呢醇消失，而代之以固体反应产物。这可能能解释1.5倍生物转化中转化水平稍微较低的原因。使用配备有更好混合装置的反应器可能会改善1.5倍生物转化中的eeh转化率。结果表明，倘若实现充分混合，则以188g/l的eeh或更高浓度运行生物转化是可能的，搅拌效率看起来是该系统的唯一限制。(-)-降龙涎醚生产力“(-)-降龙涎醚生产力”是指每小时生物转化时间(即添加底物后的时间)每升生物转化物中的可回收(-)-降龙涎醚的量(克数)。就这一点而言并且参照图22，(-)-降龙涎醚生产力计算如下：125g/l的eeh生物转化(250g/l的细胞)1.25小时时的生产力：每小时每升10.3克8.25小时时的生产力：每小时每升6.3克21.25小时时的生产力：每小时每升4.1克187.5g/l的eeh生物转化(375g/l的细胞)1.25小时时的生产力：每小时每升12.2克8.25小时时的生产力：每小时每升8.2克21.25小时时的生产力：每小时每升5.5克可以认为，在开始后约6-8小时计算的生产力代表反应的初始速度，该初始速度可最好地描述系统的最大转化速率。采用125g/l的eeh和250g/l的细胞的典型生物转化显示，在约6-8小时后降龙涎醚生产力介于6.3至8.5g每小时每升之间(代表反应的初始速度)。实施例20用nacl溶液替代反应缓冲液方法20如实施例7中所述运行常规的生物转化(125g/l的eeh、250g/l的细胞、1.55％的sds)，但是用0.5％或0.9％nacl替代柠檬酸缓冲液ph5.4，所有其它反应参数不变。平行运行在柠檬酸缓冲液中的生物转化作为对照。结果20图23中的结果证明，在缓冲液和0.9％nacl中运行的反应中eeh转化率相同。当反应在仅0.5％的nacl中运行时转化率稍低。结果证明只要保证精确的ph调节和足够的离子强度，则在不存在缓冲液的情况下运行生物转化是可能的。实施例21反应液固相的萃取考虑到(-)-降龙涎醚在水中是不可溶的并且在低于大约75℃的温度下不是液体，尽可能利用这些特性使用水混溶性溶剂(如乙醇)和水不混溶性溶剂(如甲苯)来从该生物转化的固相萃取产物。方法21将200ml反应液离心以从液(水)相分离固体(sorvallgs3，5000转/分钟，10分钟，10℃)。这使大约80ml固体沉淀物与大约120ml液相分离。在mtbe萃取后对水相进行的分析(气相色谱，实施例8)显示，其含有不超过最初存在于200ml反应液体中的(-)-降龙涎醚的大约0.3％。将甲苯和99％乙醇用于从固相萃取降龙涎醚。结果21甲苯萃取：将80ml固相用以下条件萃取6次：使用45ml甲苯(大约固相体积的一半)，剧烈振荡30秒，离心(sorvallgs3，5000转/分钟，10分钟，10℃)。用gc分析溶剂相的(-)-降龙涎醚含量。通过6次萃取超过99.5％的最初存在于反应液中的(-)-降龙涎醚被萃取，6次萃取代表总甲苯体积为最初整个反应液体积(200ml)的1.35倍或者是固相体积的3.4倍。图24中的曲线图示出了萃取随甲苯洗涤的演变，以初始存在于200ml整个反应液中的(-)-降龙涎醚量的百分比表示(由于反应液/甲苯体积比，第一次萃取中的百分比超过100％)。乙醇萃取：用大约160ml(2倍体积)99％的乙醇萃取80ml固相(inforsmultiforsht，35℃，1000转/分钟，30分钟)，然后离心。在萃取过程中降龙涎醚不结晶。图25中的曲线图显示，在4次洗涤后(总共640mletoh，即3.2倍初始的整个反应液体积或8倍固相体积)，约99％的初始存在于反应液中的降龙涎醚得以回收。在第一萃取步骤中需要足量的乙醇来防止降龙涎醚结晶(在乙醇中的溶解性)。当在第一萃取步骤中使用仅1体积或1/2体积的固相时，获得粘性糊状物，其难以处理并且在离心过程中(-)-降龙涎醚以针状结晶物结晶于离心沉淀上。温度看起来不是造成该结晶的因素(萃取和离心在室温以及大约35℃-40℃下测试)。etoh相中的(-)-降龙涎醚浓度以及液相的etoh/水比率(固相的残留水分)看起来是结晶形成的原因。然而注意到，将乙醇体积减少至1固相体积是可能的。评论21因为在室温下(-)-降龙涎醚不在液相中，其与生物质分离并且可用有机溶剂(如水混溶性溶剂(如乙醇)或非水混溶性溶剂(如甲苯))萃取。将(-)-降龙涎醚分离进反应混合物固相中的离心步骤是有利的，因为其减少了萃取(-)-降龙涎醚所需的溶剂量。实施例22感官分析目的：为了进行对“粗”萃取物和“结晶”萃取物中所形成的(-)-降龙涎醚和副产物(化合物ii、iii和iv)进行感官分析。结果22(a)eeh转化得到(-)-降龙涎醚(化合物i)和(-)-降龙涎醚异构体(化合物iv)。结果22(b)ezh生物转化得到大环醚(化合物ii)和9b-表-降龙涎醚(化合物iii)。结果22(c)粗(-)-降龙涎醚的组成包含化合物i、ii、iii和iv，各化合物分别以87.1％、2.8％、2.5％和7.6％的量存在。结果22(d)选择性结晶材料(实验室规模)的组成具有相同组分，分别以99.1％、0.1％、0.1％和0.7％的量存在。感官分析结果如下：(-)-降龙涎醚：oth0.2ng/l(oth是嗅觉阈值)。来自eeh的化合物iv：弱，isoe、木香、gc-th5-10ng。来自ezh的化合物ii：“无臭”(gc-th>500ng)(gc-th是检测阈值)。来自ezh的化合物iii：gc-th比降龙涎醚高约10倍(大约2ng)。结论“粗制”萃取物中3种副产物(化合物ii、iii和iv)每一者的总百分比约为3％。“结晶”萃取物中3种副产物(化合物ii、iii和iv)每一者的总百分比约为1％(实验室规模)。对3种副产物(化合物ii、iii和iv)的感官分析表明气味弱于来自(-)-降龙涎醚的气味。实际上，9b-表-降龙涎醚(化合物iii)气味比(-)-降龙涎醚弱约10倍，从而表明其基本上是无臭的。感官分析证明，从(-)-降龙涎醚移除一种或多种副产物化合物可改善剩余化合物((-)-降龙涎醚)的气味，即使所移除的化合物实际上本身是无臭化合物。也就是说，在不存在化合物ii、iii和iv的情况下观察到降龙涎醚气味增强。实施例23通过汽提回收降龙涎醚方法23所得的粗制(汽提)和结晶(-)-降龙涎醚的纯度汽提ee:ez86:14生物转化反应混合物并如下对反应产物进行结晶：收集蒸气溜出液为两相混合物。保留有机相，弃去水相。通过gc分析有机相的组成，结果在下面的表25中示出(见“粗制”)。然后浓缩有机相至干燥。然后将乙醇加至该粗制干燥产品并使该混合物升温直至产品溶解。室温下缓慢添加水，(-)-降龙涎醚在偶尔搅拌和冰浴中冷却下结晶。结果23下面的表25还示出了在汽提/蒸馏步骤(“粗制”)后获得的产品及结晶产品((-)-降龙涎醚)的gc分析结果。表25中提及“ezh”和“eeh”分别指(3z,7e)高法呢醇和7e,3e高法呢醇。表25表明，使用wtshc或shc衍生物，特定的原料(eeh:ezh86:14)产生了所需的终产物(-)-降龙涎醚和十分特别的副产物混合物(ii、iv和iii)。选择性结晶的数据显示了(-)降龙涎醚(i)的强烈富集，在结晶样品中几乎不存在副产物(ii)、(iv)或(iii)。因此，这种ee:ez混合物提供了在嗅觉上纯的(-)-降龙涎醚产品，该产品以相对直接且成本低的方式选择性结晶。表25：示出了结晶产品的gc分析结果.讨论23汽提/过滤是分离降龙涎醚的环境友好的方法，因为其提供了对降龙涎醚的便捷无溶剂分离，没有相关的生物催化剂失活。总结23采用生物转化反应制备的(-)-降龙涎醚可利用本领域技术人员已知的方法，用溶剂从整个反应混合物萃取(如使用非水混溶性溶剂或通过汽提/蒸馏或通过过滤)或从固相萃取(如使用水混溶性溶剂)。序列表<110>givaudansa<120>酶及其应用<130>30578<150>gb1507207.7<151>2015-04-24<160>188<170>patentinversion3.3<210>1<211>631<212>prt<213>酸热脂环酸芽孢杆菌（alicyclobacillusacidocaldarius）<400>1metalagluglnleuvalglualaproalatyralaargthrleuasp151015argalavalglutyrleuleusercysglnlysaspgluglytyrtrp202530trpglyproleuleuserasnvalthrmetglualaglutyrvalleu354045leucyshisileleuaspargvalaspargaspargmetglulysile505560argargtyrleuleuhisgluglnarggluaspglythrtrpalaleu65707580tyrproglyglyproproaspleuaspthrthrileglualatyrval859095alaleulystyrileglymetserargaspglugluprometglnlys100105110alaleuargpheileglnserglnglyglyilegluserserargval115120125phethrargmettrpleualaleuvalglyglutyrprotrpglulys130135140valprometvalproprogluilemetpheleuglylysargmetpro145150155160leuasniletyrglupheglysertrpalaargalathrvalvalala165170175leuserilevalmetserargglnprovalpheproleuprogluarg180185190alaargvalprogluleutyrgluthraspvalproproargargarg195200205glyalalysglyglyglyglytrpilepheaspalaleuaspargala210215220leuhisglytyrglnlysleuservalhispropheargargalaala225230235240gluileargalaleuasptrpleuleugluargglnalaglyaspgly245250255sertrpglyglyileglnproprotrpphetyralaleuilealaleu260265270lysileleuaspmetthrglnhisproalapheilelysglytrpglu275280285glyleugluleutyrglyvalgluleuasptyrglyglytrpmetphe290295300glnalaserileserprovaltrpaspthrglyleualavalleuala305310315320leuargalaalaglyleuproalaasphisaspargleuvallysala325330335glyglutrpleuleuaspargglnilethrvalproglyasptrpala340345350vallysargproasnleulysproglyglyphealapheglnpheasp355360365asnvaltyrtyrproaspvalaspaspthralavalvalvaltrpala370375380leuasnthrleuargleuproaspgluargargargargaspalamet385390395400thrlysglypheargtrpilevalglymetglnserserasnglygly405410415trpglyalatyraspvalaspasnthrseraspleuproasnhisile420425430prophecysasppheglygluvalthraspproprosergluaspval435440445thralahisvalleuglucyspheglyserpheglytyraspaspala450455460trplysvalileargargalavalglutyrleulysarggluglnlys465470475480proaspglysertrppheglyargtrpglyvalasntyrleutyrgly485490495thrglyalavalvalseralaleulysalavalglyileaspthrarg500505510gluprotyrileglnlysalaleuasptrpvalgluglnhisglnasn515520525proaspglyglytrpglygluaspcysargsertyrgluaspproala530535540tyralaglylysglyalaserthrproserglnthralatrpalaleu545550555560metalaleuilealaglyglyargalagluserglualaalaargarg565570575glyvalglntyrleuvalgluthrglnargproaspglyglytrpasp580585590gluprotyrtyrthrglythrglypheproglyaspphetyrleugly595600605tyrthrmettyrarghisvalpheprothrleualaleuglyargtyr610615620lysglnalailegluargarg625630<210>2<211>725<212>prt<213>运动发酵单胞菌（zymomonasmobilis）<400>2metglyileaspargmetasnserleuserargleuleumetlyslys151015ilepheglyalaglulysthrsertyrlysproalaseraspthrile202530ileglythraspthrleulysargproasnargargprogluprothr354045alalysvalasplysthrilephelysthrmetglyasnserleuasn505560asnthrleuvalseralacysasptrpleuileglyglnglnlyspro65707580aspglyhistrpvalglyalavalgluserasnalasermetgluala859095glutrpcysleualaleutrppheleuglyleugluasphisproleu100105110argproargleuglyasnalaleuleuglumetglnarggluaspgly115120125se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yleualavalleuala305310315320leuargalaalaglyleuproalaasphisaspargleuvallysala325330335glyglutrpleuleuaspargglnilethrvalproglyasptrpala340345350vallysargproasnleulysproglyglyphealapheglnpheasp355360365asnvaltyrtyrproaspvalaspaspthralavalvalvaltrpala370375380leuasnserleuargleuproaspgluargargargargaspvalmet385390395400thrlysglypheargtrpilevalglymetglnserserasnglygly405410415trpglyalatyraspvalaspasnthrseraspleuproasnhisile420425430prophecysasppheglygluvalthraspproprosergluaspval435440445thralahisvalleuglucyspheglyserpheglytyraspaspala450455460trplysvalileargargalavalglutyrleulysarggluglnarg465470475480proaspglysertrppheglyargtrpglyvalasntyrleutyrgly485490495thrglyalavalvalproalaleulysalavalglyileaspvalarg500505510glupropheileglnlysalaleuasptrpvalgluglnhisglnasn515520525proaspglyglytrpglygluaspcysargsertyrgluaspproala530535540tyralaglylysglyalaserthrproserglnthralatrpalaleu545550555560metalaleuilealaglyglyargalagluseraspservalargarg565570575glyvalglntyrleuvalgluthrglnargproaspglyglytrpasp580585590gluprotyrtyrthrglythrglypheproglyaspphetyrleugly595600605tyrthrmettyrarghisvalpheprothrleualaleuglyargtyr610615620lysglnalailegluargarg625630<210>179<211>382<212>dna<213>人工序列<220><223>合成质粒pet-28a(+)克隆/表达区<220><221>cds<222>(109)..(285)<400>179agatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaa60ttcccctctagaaataatttttgtttaactttaagaaggagatataccatgggcagc117metglyser1agccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagc165serhishishishishishisserserglyleuvalproargglyser51015catatggctagcatgactggtggacagcaaatgggtcgcggatccgaa213hismetalasermetthrglyglyglnglnmetglyargglyserglu20253035ttcgagctccgtcgacaagcttgcggccgcactcgagcaccaccacca261phegluleuargargglnalacysglyargthrargalapropropro404550ccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgc315proproleuargserglycys55tgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgagggg375ttttttg382<210>180<211>58<212>prt<213>人工序列<220><223>合成构建体<400>180metglyserserhishishishishishisserserglyleuvalpro151015argglyserhismetalasermetthrglyglyglnglnmetglyarg202530glysergluphegluleuargargglnalacysglyargthrargala354045proproproproproleuargserglycys5055<210>181<211>94<212>dna<213>人工序列<220><223>合成质粒pet-28b(+)克隆/表达区<220><221>cds<222>(1)..(72)<400>181ggtcgggatccgaattcgagctccgtcgacaagcttgcggccgcactc48glyargaspproasnserserservalasplysleualaalaalaleu151015gagcaccaccaccaccaccactgagatccggctgctaacaaagccc94gluhishishishishishis20<210>182<211>23<212>prt<213>人工序列<220><223>合成构建体<400>182glyargaspproasnserserservalasplysleualaalaalaleu151015gluhishishishishishis20<210>183<211>93<212>dna<213>人工序列<220><223>合成质粒pet-28c(+)克隆/表达区<220><221>cds<222>(1)..(93)<400>183ggtcggatccgaattcgagctccgtcgacaagcttgcggccgcactcg48glyargileargileargalaproserthrserleuargprohisser151015agcaccaccaccaccaccactgagatccggctgctaacaaagccc93serthrthrthrthrthrthrgluileargleuleuthrlyspro202530<210>184<211>31<212>prt<213>人工序列<220><223>合成构建体<400>184glyargileargileargalaproserthrserleuargprohisser151015serthrthrthrthrthrthrgluileargleuleuthrlyspro202530<210>185<211>631<212>prt<213>人工序列<220><223>合成shc衍生物<220><221>变体<222>(77)..(77)<223>t77x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y<220><221>变体<222>(92)..(92)<223>i92x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y<220><221>变体<222>(129)..(129)<223>f129x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(132)..(132)<223>m132x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(224)..(224)<223>a224x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(432)..(432)<223>i432x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(579)..(579)<223>q579x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(601)..(601)<223>f601x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<400>185metalagluglnleuvalglualaproalatyralaargthrleuasp151015argalavalglutyrleuleusercysglnlysaspgluglytyrtrp202530trpglyproleuleuserasnvalthrmetglualaglutyrvalleu354045leucyshisileleuaspargvalaspargaspargmetglulysile505560argargtyrleuleuhisgluglnarggluaspglyxaatrpalaleu65707580tyrproglyglyproproaspleuaspthrthrxaaglualatyrval859095alaleulystyrileglymetserargaspglugluprometglnlys100105110alaleuargpheileglnserglnglyglyilegluserserargval115120125xaathrargxaatrpleualaleuvalglyglutyrprotrpglulys130135140valprometvalproprogluilemetpheleuglylysargmetpro145150155160leuasniletyrglupheglysertrpalaargalathrvalvalala165170175leuserilevalmetserargglnprovalpheproleuprogluarg180185190alaargvalprogluleutyrgluthraspvalproproargargarg195200205glyalalysglyglyglyglytrpilepheaspalaleuaspargxaa210215220leuhisglytyrglnlysleuservalhispropheargargalaala225230235240gluileargalaleuasptrpleuleugluargglnalaglyaspgly245250255sertrpglyglyileglnproprotrpphetyralaleuilealaleu260265270lysileleuaspmetthrglnhisproalapheilelysglytrpglu275280285glyleugluleutyrglyvalgluleuasptyrglyglytrpmetphe290295300glnalaserileserprovaltrpaspthrglyleualavalleuala305310315320leuargalaalaglyleuproalaasphisaspargleuvallysala325330335glyglutrpleuleuaspargglnilethrvalproglyasptrpala340345350vallysargproasnleulysproglyglyphealapheglnpheasp355360365asnvaltyrtyrproaspvalaspaspthralavalvalvaltrpala370375380leuasnthrleuargleuproaspgluargargargargaspalamet385390395400thrlysglypheargtrpilevalglymetglnserserasnglygly405410415trpglyalatyraspvalaspasnthrseraspleuproasnhisxaa420425430prophecysasppheglygluvalthraspproprosergluaspval435440445thralahisvalleuglucyspheglyserpheglytyraspaspala450455460trplysvalileargargalavalglutyrleulysarggluglnlys465470475480proaspglysertrppheglyargtrpglyvalasntyrleutyrgly485490495thrglyalavalvalseralaleulysalavalglyileaspthrarg500505510gluprotyrileglnlysalaleuasptrpvalgluglnhisglnasn515520525proaspglyglytrpglygluaspcysargsertyrgluaspproala530535540tyralaglylysglyalaserthrproserglnthralatrpalaleu545550555560metalaleuilealaglyglyargalagluserglualaalaargarg565570575glyvalxaatyrleuvalgluthrglnargproaspglyglytrpasp580585590gluprotyrtyrthrglythrglyxaaproglyaspphetyrleugly595600605tyrthrmettyrarghisvalpheprothrleualaleuglyargtyr610615620lysglnalailegluargarg625630<210>186<211>725<212>prt<213>人工序列<220><223>合成shc衍生物<220><221>变体<222>(129)..(129)<223>s129x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(145)..(145)<223>v145x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(182)..(182)<223>f182x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(185)..(185)<223>y185x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(282)..(282)<223>g282x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(498)..(498)<223>i498x中的x选自:a,b,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(646)..(646)<223>h646x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(668)..(668)<223>f668x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<400>186metglyileaspargmetasnserleuserargleuleumetlyslys151015ilepheglyalaglulysthrsertyrlysproalaseraspthrile202530ileglythraspthrleulysargproasnargargprogluprothr354045alalysvalasplysthrilephelysthrmetglyasnserleuasn505560asnthrleuvalseralacysasptrpleuileglyglnglnlyspro65707580aspglyhistrpvalglyalavalgluserasnalasermetgluala859095glutrpcysleualaleutrppheleuglyleugluasphisproleu100105110argproargleuglyasnalaleuleuglumetglnarggluaspgly115120125xaatrpglyvaltyrpheglyalaglyasnglyaspileasnalathr130135140xaaglualatyralaalaleuargserleuglytyrseralaaspasn145150155160provalleulyslysalaalaalatrpilealaglulysglyglyleu165170175lysasnileargvalxaathrargxaatrpleualaleuileglyglu180185190trpprotrpglulysthrproasnleuproprogluileiletrpphe195200205proaspasnphevalpheseriletyrasnphealaglntrpalaarg210215220alathrmetvalproilealaileleuseralaargargproserarg225230235240proleuargproglnaspargleuaspgluleupheprogluglyarg245250255alaargpheasptyrgluleuprolyslysgluglyileaspleutrp260265270serglnphepheargthrthraspargxaaleuhistrpvalglnser275280285asnleuleulysargasnserleuargglualaalailearghisval290295300leuglutrpileilearghisglnaspalaaspglyglytrpglygly305310315320ileglnproprotrpvaltyrglyleumetalaleuhisglyglugly325330335tyrglnleutyrhisprovalmetalalysalaleuseralaleuasp340345350aspproglytrparghisaspargglyglusersertrpileglnala355360365thrasnserprovaltrpaspthrmetleualaleumetalaleulys370375380aspalalysalagluaspargphethrproglumetasplysalaala385390395400asptrpleuleualaargglnvallysvallysglyasptrpserile405410415lysleuproaspvalgluproglyglytrpalapheglutyralaasn420425430aspargtyrproaspthraspaspthralavalalaleuilealaleu435440445sersertyrargasplysgluglutrpglnlyslysglyvalgluasp450455460alailethrargglyvalasntrpleuilealametglnserglucys465470475480glyglytrpglyalapheasplysaspasnasnargserileleuser485490495lysxaaprophecysasppheglygluserileaspproproserval500505510aspvalthralahisvalleuglualapheglythrleuglyleuser515520525argaspmetprovalileglnlysalaileasptyrvalargserglu530535540glnglualagluglyalatrppheglyargtrpglyvalasntyrile545550555560tyrglythrglyalavalleuproalaleualaalaileglygluasp565570575metthrglnprotyrilethrlysalacysasptrpleuvalalahis580585590glnglngluaspglyglytrpglyglusercyssersertyrmetglu595600605ileaspserileglylysglyprothrthrproserglnthralatrp610615620alaleumetglyleuilealaalaasnargprogluasptyrgluala625630635640ilealalysglycysxaatyrleuileaspargglngluglnaspgly645650655sertrplysgluglugluphethrglythrglyxaaproglytyrgly660665670valglyglnthrilelysleuaspaspproalaleuserlysargleu675680685leuglnglyalagluleuserargalaphemetleuargtyraspphe690695700tyrargglnphepheproilemetalaleuserargalagluargleu705710715720ileaspleuasnasn725<210>187<211>658<212>prt<213>人工序列<220><223>合成shc衍生物<220><221>变体<222>(85)..(85)<223>g85x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(100)..(100)<223>v100x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(137)..(137)<223>f137x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(140)..(140)<223>i140x中的x选自:a,,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(233)..(233)<223>v233x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(450)..(450)<223>i450x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(598)..(598)<223>n598x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y<220><221>变体<222>(620)..(620)<223>f620x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<400>187metthrvalserthrserseralaphehishisserproleuserasp151015aspvalgluproileileglnlysalathrargalaleuleuglulys202530glnglnglnaspglyhistrpvalphegluleuglualaaspalathr354045ileproalaglutyrileleuleulyshistyrleuglygluproglu505560aspleugluileglualalysileglyargtyrleuargargilegln65707580glygluhisglyxaatrpserleuphetyrglyglyaspleuaspleu859095seralathrxaalysalatyrphealaleulysmetileglyaspser100105110proaspalaprohismetleuargalaargasngluileleualaarg115120125glyglyalametargalaasnvalxaathrargxaaglnleualaleu130135140pheglyalametsertrpgluhisvalproglnmetprovalgluleu145150155160metleumetproglutrppheprovalhisileasnlysmetalatyr165170175trpalaargthrvalleuvalproleuleuvalleuglnalaleulys180185190provalalaargasnargargglyileleuvalaspgluleupheval195200205proaspvalleuprothrleuglngluserglyaspproiletrparg210215220argphepheseralaleuasplysxaaleuhislysvalgluprotyr225230235240trpprolysasnmetargalalysalailehissercysvalhisphe245250255valthrgluargleuasnglygluaspglyleuglyalailetyrpro260265270alailealaasnservalmetmettyraspalaleuglytyrproglu275280285asnhisprogluargalailealaargargalavalglulysleumet290295300valleuaspglythrgluaspglnglyasplysgluvaltyrcysgln305310315320procysleuserproiletrpaspthralaleuvalalahisalamet325330335leugluvalglyglyaspglualaglulysseralaileseralaleu340345350sertrpleulysproglnglnileleuaspvallysglyasptrpala355360365trpargargproaspleuargproglyglytrpalapheglntyrarg370375380asnasptyrtyrproaspvalaspaspthralavalvalthrmetala385390395400metaspargalaalalysleuseraspleuhisaspaspphegluglu405410415serlysalaargalametglutrpthrileglymetglnseraspasn420425430glyglytrpglyalapheaspalaasnasnsertyrthrtyrleuasn435440445asnxaaprophealaasphisglyalaleuleuaspproprothrval450455460aspvalseralaargcysvalsermetmetalaglnalaglyileser465470475480ilethraspprolysmetlysalaalavalasptyrleuleulysglu485490495glnglugluaspglysertrppheglyargtrpglyvalasntyrile500505510tyrglythrtrpseralaleucysalaleuasnvalalaalaleupro515520525hisasphisleualavalglnlysalavalalatrpleulysthrile530535540glnasngluaspglyglytrpglygluasncysaspsertyralaleu545550555560asptyrserglytyrgluprometaspserthralaserglnthrala565570575trpalaleuleuglyleumetalavalglyglualaasnsergluala580585590valthrlysglyilexaatrpleualaglnasnglnaspgluglugly595600605leutrplysgluasptyrtyrserglyglyglyxaaproargvalphe610615620tyrleuargtyrhisglytyrserlystyrpheproleutrpalaleu625630635640alaargtyrargasnleulyslysalaasnglnproilevalhistyr645650655glymet<210>188<211>684<212>prt<213>人工序列<220><223>合成shc衍生物<220><221>变体<222>(88)..(88)<223>a88x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(104)..(104)<223>v104x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(141)..(141)<223>f141x,中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(144)..(144)<223>y144x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(241)..(241)<223>v241x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(459)..(459)<223>i459x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(607)..(607)<223>m607x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<220><221>变体<222>(628)..(628)<223>f628x中的x选自:a,c,d,e,f,g,h,i,k,l,m,n,p,q,r,s,t,v,w或y.<400>188metthrvalthrserseralaseralaargalathrargaspprogly151015asntyrglnthralaleuglnserthrvalargalaalaalaasptrp202530leuilealaasnglnlysproaspglyhistrpvalglyargalaglu354045serasnalacysmetglualaglntrpcysleualaleutrpphemet505560glyleugluasphisproleuarglysargleuglyglnserleuleu65707580aspserglnargproaspglyxaatrpglnvaltyrpheglyalapro859095asnglyaspileasnalathrxaaglualatyralaalaleuargser100105110leuglypheargaspaspgluproalavalargargalaargglutrp115120125ileglualalysglyglyleuargasnileargvalxaathrargxaa130135140trpleualaleuileglyglutrpprotrpglulysthrproasnile145150155160proprogluvaliletrppheproleutrpphepropheseriletyr165170175asnphealaglntrpalaargalathrleumetproilealavalleu180185190seralaargargproserargproleuproprogluasnargleuasp195200205alaleupheprohisglyarglysalapheasptyrgluleuproval210215220lysalaglyalaglyglytrpaspargphepheargglyalaasplys225230235240xaaleuhislysleuglnasnleuglyasnargleuasnleuglyleu245250255pheargproalaalathrserargvalleuglutrpmetilearghis260265270glnasppheaspglyalatrpglyglyileglnproprotrpiletyr275280285glyleumetalaleutyralagluglytyrproleuasnhisproval290295300leualalysglyleuaspalaleuasnaspproglytrpargvalasp305310315320valglyaspalathrtyrileglnalathrasnserprovaltrpasp325330335thrileleuthrleuleualapheaspaspalaglyvalleuglyasp340345350tyrproglualavalasplysalavalasptrpvalleuglnarggln355360365valargvalproglyasptrpsermetlysleuprohisvallyspro370375380glyglytrpalapheglutyralaasnasntyrtyrproaspthrasp385390395400aspthralavalalaleuilealaleualaproleuarghisasppro405410415lystrplysalalysglyileaspglualaileglnleuglyvalasp420425430trpleuileglymetglnserglnglyglyglytrpglyalapheasp435440445lysaspasnasnglnlysileleuthrlysxaaprophecysasptyr450455460glyglualaleuaspproproservalaspvalthralahisileile465470475480glualapheglylysleuglyileserargasnhisprosermetval485490495glnalaleuasptyrileargarggluglngluproserglyprotrp500505510pheglyargtrpglyvalasntyrvaltyrglythrglyalavalleu515520525proalaleualaalaileglygluaspmetthrglnprotyrilegly530535540argalacysasptrpleuvalalahisglnglnalaaspglyglytrp545550555560glyglusercysalasertyrmetaspvalseralavalglyarggly565570575thrthrthralaserglnthralatrpalaleumetalaleuleuala580585590alaasnargproglnasplysaspalailegluargglycysxaatrp595600605leuvalgluargglnseralaglythrtrpaspgluprogluphethr610615620glythrglyxaaproglytyrglyvalglyglnthrilelysleuasn625630635640aspproalaleuserglnargleumetglnglyprogluleuserarg645650655alaphemetleuargtyrglymettyrarghistyrpheproleumet660665670alaleuglyargalaleuargproglnserhisser675680当前第1页12